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miR-381 Regulates Neural Stem Cell Proliferation and Differentiation via Regulating Hes1 Expression.

Shi X, Yan C, Liu B, Yang C, Nie X, Wang X, Zheng J, Wang Y, Zhu Y - PLoS ONE (2015)

Bottom Line: Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells.Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons.In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.

ABSTRACT
Neural stem cells are self-renewing, multipotent and undifferentiated precursors that retain the capacity for differentiation into both glial (astrocytes and oligodendrocytes) and neuronal lineages. Neural stem cells offer cell-based therapies for neurological disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease and spinal cord injuries. However, their cellular behavior is poorly understood. MicroRNAs (miRNAs) are a class of small noncoding RNAs involved in cell development, proliferation and differentiation through regulating gene expression at post-transcriptional level. The role of miR-381 in the development of neural stem cells remains unknown. In this study, we showed that overexpression of miR-381 promoted neural stem cells proliferation. It induced the neural stem cells differentiation to neurons and inhibited their differentiation to astrocytes. Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells. Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons. In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

No MeSH data available.


Related in: MedlinePlus

Hes1 was the direct target of miR–381 in neural stem cells.(A) Hes1 was predicted to be target gene ofmiR–381 by TargetScan. (B) Luciferase reporter assay was done to confirm the predictions in neural stem cells. (C) The protein expression of Hes1 was measured by Western blot in neural stem cells.***p<0.001.
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pone.0138973.g005: Hes1 was the direct target of miR–381 in neural stem cells.(A) Hes1 was predicted to be target gene ofmiR–381 by TargetScan. (B) Luciferase reporter assay was done to confirm the predictions in neural stem cells. (C) The protein expression of Hes1 was measured by Western blot in neural stem cells.***p<0.001.

Mentions: Hes1 was predicted to be target gene ofmiR–381 by TargetScan (Fig 5A).As shown in Fig 5B,miR-381repressed the luciferase activity of wild type 3’UTRof Hes1vector compared to that mutant 3’UTR of Hes1 vector (Fig 5B). Overexpression of miR–381 inhibited HES1 protein expression (Fig 5C).


miR-381 Regulates Neural Stem Cell Proliferation and Differentiation via Regulating Hes1 Expression.

Shi X, Yan C, Liu B, Yang C, Nie X, Wang X, Zheng J, Wang Y, Zhu Y - PLoS ONE (2015)

Hes1 was the direct target of miR–381 in neural stem cells.(A) Hes1 was predicted to be target gene ofmiR–381 by TargetScan. (B) Luciferase reporter assay was done to confirm the predictions in neural stem cells. (C) The protein expression of Hes1 was measured by Western blot in neural stem cells.***p<0.001.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591969&req=5

pone.0138973.g005: Hes1 was the direct target of miR–381 in neural stem cells.(A) Hes1 was predicted to be target gene ofmiR–381 by TargetScan. (B) Luciferase reporter assay was done to confirm the predictions in neural stem cells. (C) The protein expression of Hes1 was measured by Western blot in neural stem cells.***p<0.001.
Mentions: Hes1 was predicted to be target gene ofmiR–381 by TargetScan (Fig 5A).As shown in Fig 5B,miR-381repressed the luciferase activity of wild type 3’UTRof Hes1vector compared to that mutant 3’UTR of Hes1 vector (Fig 5B). Overexpression of miR–381 inhibited HES1 protein expression (Fig 5C).

Bottom Line: Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells.Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons.In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.

ABSTRACT
Neural stem cells are self-renewing, multipotent and undifferentiated precursors that retain the capacity for differentiation into both glial (astrocytes and oligodendrocytes) and neuronal lineages. Neural stem cells offer cell-based therapies for neurological disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease and spinal cord injuries. However, their cellular behavior is poorly understood. MicroRNAs (miRNAs) are a class of small noncoding RNAs involved in cell development, proliferation and differentiation through regulating gene expression at post-transcriptional level. The role of miR-381 in the development of neural stem cells remains unknown. In this study, we showed that overexpression of miR-381 promoted neural stem cells proliferation. It induced the neural stem cells differentiation to neurons and inhibited their differentiation to astrocytes. Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells. Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons. In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

No MeSH data available.


Related in: MedlinePlus