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miR-381 Regulates Neural Stem Cell Proliferation and Differentiation via Regulating Hes1 Expression.

Shi X, Yan C, Liu B, Yang C, Nie X, Wang X, Zheng J, Wang Y, Zhu Y - PLoS ONE (2015)

Bottom Line: Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells.Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons.In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.

ABSTRACT
Neural stem cells are self-renewing, multipotent and undifferentiated precursors that retain the capacity for differentiation into both glial (astrocytes and oligodendrocytes) and neuronal lineages. Neural stem cells offer cell-based therapies for neurological disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease and spinal cord injuries. However, their cellular behavior is poorly understood. MicroRNAs (miRNAs) are a class of small noncoding RNAs involved in cell development, proliferation and differentiation through regulating gene expression at post-transcriptional level. The role of miR-381 in the development of neural stem cells remains unknown. In this study, we showed that overexpression of miR-381 promoted neural stem cells proliferation. It induced the neural stem cells differentiation to neurons and inhibited their differentiation to astrocytes. Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells. Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons. In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

No MeSH data available.


Related in: MedlinePlus

miR–381 promoted neural stem cells differentiation to neurons.(A) Immunocytochemical staining of purified neurons with β-tubulin-III. (B) The mRNA expression of β-tubulin-III was detected by qRT-PCR. (C) The protein expression of β-tubulin-III was measured by Western blot.***p<0.001.
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pone.0138973.g003: miR–381 promoted neural stem cells differentiation to neurons.(A) Immunocytochemical staining of purified neurons with β-tubulin-III. (B) The mRNA expression of β-tubulin-III was detected by qRT-PCR. (C) The protein expression of β-tubulin-III was measured by Western blot.***p<0.001.

Mentions: miR–381 promoted neural stem cells differentiation to neurons as confirmed by immunofluorescence (Fig 3A). miR–381 overexpression promoted the mRNA and protein expression of β-tubulin III (Fig 3B and 3C).


miR-381 Regulates Neural Stem Cell Proliferation and Differentiation via Regulating Hes1 Expression.

Shi X, Yan C, Liu B, Yang C, Nie X, Wang X, Zheng J, Wang Y, Zhu Y - PLoS ONE (2015)

miR–381 promoted neural stem cells differentiation to neurons.(A) Immunocytochemical staining of purified neurons with β-tubulin-III. (B) The mRNA expression of β-tubulin-III was detected by qRT-PCR. (C) The protein expression of β-tubulin-III was measured by Western blot.***p<0.001.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591969&req=5

pone.0138973.g003: miR–381 promoted neural stem cells differentiation to neurons.(A) Immunocytochemical staining of purified neurons with β-tubulin-III. (B) The mRNA expression of β-tubulin-III was detected by qRT-PCR. (C) The protein expression of β-tubulin-III was measured by Western blot.***p<0.001.
Mentions: miR–381 promoted neural stem cells differentiation to neurons as confirmed by immunofluorescence (Fig 3A). miR–381 overexpression promoted the mRNA and protein expression of β-tubulin III (Fig 3B and 3C).

Bottom Line: Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells.Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons.In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.

ABSTRACT
Neural stem cells are self-renewing, multipotent and undifferentiated precursors that retain the capacity for differentiation into both glial (astrocytes and oligodendrocytes) and neuronal lineages. Neural stem cells offer cell-based therapies for neurological disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease and spinal cord injuries. However, their cellular behavior is poorly understood. MicroRNAs (miRNAs) are a class of small noncoding RNAs involved in cell development, proliferation and differentiation through regulating gene expression at post-transcriptional level. The role of miR-381 in the development of neural stem cells remains unknown. In this study, we showed that overexpression of miR-381 promoted neural stem cells proliferation. It induced the neural stem cells differentiation to neurons and inhibited their differentiation to astrocytes. Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells. Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons. In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

No MeSH data available.


Related in: MedlinePlus