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miR-381 Regulates Neural Stem Cell Proliferation and Differentiation via Regulating Hes1 Expression.

Shi X, Yan C, Liu B, Yang C, Nie X, Wang X, Zheng J, Wang Y, Zhu Y - PLoS ONE (2015)

Bottom Line: Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells.Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons.In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.

ABSTRACT
Neural stem cells are self-renewing, multipotent and undifferentiated precursors that retain the capacity for differentiation into both glial (astrocytes and oligodendrocytes) and neuronal lineages. Neural stem cells offer cell-based therapies for neurological disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease and spinal cord injuries. However, their cellular behavior is poorly understood. MicroRNAs (miRNAs) are a class of small noncoding RNAs involved in cell development, proliferation and differentiation through regulating gene expression at post-transcriptional level. The role of miR-381 in the development of neural stem cells remains unknown. In this study, we showed that overexpression of miR-381 promoted neural stem cells proliferation. It induced the neural stem cells differentiation to neurons and inhibited their differentiation to astrocytes. Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells. Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons. In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

No MeSH data available.


Related in: MedlinePlus

miR–381 promoted neural stem cells proliferation.(A) The expression of miR–381 was measured by qRT-PCR. (B) CCK–8 was performed to detect the neural stem cells proliferation. (C) The mRNA expression of nestin was detected by qRT-PCR. (D) The protein expression of nestin was measured by Western blot. **p<0.01 and ***p<0.001.
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pone.0138973.g002: miR–381 promoted neural stem cells proliferation.(A) The expression of miR–381 was measured by qRT-PCR. (B) CCK–8 was performed to detect the neural stem cells proliferation. (C) The mRNA expression of nestin was detected by qRT-PCR. (D) The protein expression of nestin was measured by Western blot. **p<0.01 and ***p<0.001.

Mentions: We confirmed that miR–381 mimics could promote the expression of miR–381 in neural stem cells (Fig 2A). CCK–8 analysis demonstrated that overexpression of miR–381 increased neural stem cells proliferation (Fig 2B). In addition, miR–381 overexpression promoted the mRNA and protein expression of nestin (Fig 2C and 2D).


miR-381 Regulates Neural Stem Cell Proliferation and Differentiation via Regulating Hes1 Expression.

Shi X, Yan C, Liu B, Yang C, Nie X, Wang X, Zheng J, Wang Y, Zhu Y - PLoS ONE (2015)

miR–381 promoted neural stem cells proliferation.(A) The expression of miR–381 was measured by qRT-PCR. (B) CCK–8 was performed to detect the neural stem cells proliferation. (C) The mRNA expression of nestin was detected by qRT-PCR. (D) The protein expression of nestin was measured by Western blot. **p<0.01 and ***p<0.001.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591969&req=5

pone.0138973.g002: miR–381 promoted neural stem cells proliferation.(A) The expression of miR–381 was measured by qRT-PCR. (B) CCK–8 was performed to detect the neural stem cells proliferation. (C) The mRNA expression of nestin was detected by qRT-PCR. (D) The protein expression of nestin was measured by Western blot. **p<0.01 and ***p<0.001.
Mentions: We confirmed that miR–381 mimics could promote the expression of miR–381 in neural stem cells (Fig 2A). CCK–8 analysis demonstrated that overexpression of miR–381 increased neural stem cells proliferation (Fig 2B). In addition, miR–381 overexpression promoted the mRNA and protein expression of nestin (Fig 2C and 2D).

Bottom Line: Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells.Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons.In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, PR China.

ABSTRACT
Neural stem cells are self-renewing, multipotent and undifferentiated precursors that retain the capacity for differentiation into both glial (astrocytes and oligodendrocytes) and neuronal lineages. Neural stem cells offer cell-based therapies for neurological disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease and spinal cord injuries. However, their cellular behavior is poorly understood. MicroRNAs (miRNAs) are a class of small noncoding RNAs involved in cell development, proliferation and differentiation through regulating gene expression at post-transcriptional level. The role of miR-381 in the development of neural stem cells remains unknown. In this study, we showed that overexpression of miR-381 promoted neural stem cells proliferation. It induced the neural stem cells differentiation to neurons and inhibited their differentiation to astrocytes. Furthermore, we identified HES1 as a direct target of miR-381 in neural stem cells. Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons. In conclusion, miR-381 played important role in neural stem cells proliferation and differentiation.

No MeSH data available.


Related in: MedlinePlus