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Screening for biomarkers of liver injury induced by Polygonum multiflorum: a targeted metabolomic study.

Dong Q, Li N, Li Q, Zhang CE, Feng WW, Li GQ, Li RY, Tu C, Han X, Bai ZF, Zhang YM, Niu M, Ma ZJ, Xiao XH, Wang JB - Front Pharmacol (2015)

Bottom Line: Heshouwu (HSW), the dry roots of Polygonum multiflorum, a classical traditional Chinese medicine is used as a tonic for a wide range of conditions, particularly those associated with aging.Nine BAs including cholic acid (CA) and chenodeoxycholic acid (CDCA), taurocholic acid (TCA), glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), deoxycholic acid (DCA), glycodeoxycholic acid (GDCA), ursodeoxycholic acid (UDCA), and hyodeoxycholic acid (HDCA) in rat bile and serum were detected by a developed LC-MS method after 42 days treatment.Partial least square-discriminate analysis (PLS-DA) was applied to evaluate the indicative roles of the nine BAs, and metabolism of the nine BAs was summarized.

View Article: PubMed Central - PubMed

Affiliation: China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China ; College of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu, China.

ABSTRACT
Heshouwu (HSW), the dry roots of Polygonum multiflorum, a classical traditional Chinese medicine is used as a tonic for a wide range of conditions, particularly those associated with aging. However, it tends to be taken overdose or long term in these years, which has resulted in liver damage reported in many countries. In this study, the indicative roles of nine bile acids (BAs) were evaluated to offer potential biomarkers for HSW induced liver injury. Nine BAs including cholic acid (CA) and chenodeoxycholic acid (CDCA), taurocholic acid (TCA), glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), deoxycholic acid (DCA), glycodeoxycholic acid (GDCA), ursodeoxycholic acid (UDCA), and hyodeoxycholic acid (HDCA) in rat bile and serum were detected by a developed LC-MS method after 42 days treatment. Partial least square-discriminate analysis (PLS-DA) was applied to evaluate the indicative roles of the nine BAs, and metabolism of the nine BAs was summarized. Significant change was observed for the concentrations of nine BAs in treatment groups compared with normal control; In the PLS-DA plots of nine BAs in bile, normal control and raw HSW groups were separately clustered and could be clearly distinguished, GDCA was selected as the distinguished components for raw HSW overdose treatment group. In the PLS-DA plots of nine BAs in serum, the normal control and raw HSW overdose treatment group were separately clustered and could be clearly distinguished, and HDCA was selected as the distinguished components for raw HSW overdose treatment group. The results indicated the perturbation of nine BAs was associated with HSW induced liver injury; GDCA in bile, as well as HDCA in serum could be selected as potential biomarkers for HSW induced liver injury; it also laid the foundation for the further search on the mechanisms of liver injury induced by HSW.

No MeSH data available.


Related in: MedlinePlus

Discrimination of normal control rats from raw HSW, processed HSW and chlorpromazine treatment rats according to PLS-DA. (A–C) Discriminations based on the quantitative analysis of BAs in rat bile. (D–F) Discriminations based on the quantitative analysis of BAs in rat serum.
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Figure 3: Discrimination of normal control rats from raw HSW, processed HSW and chlorpromazine treatment rats according to PLS-DA. (A–C) Discriminations based on the quantitative analysis of BAs in rat bile. (D–F) Discriminations based on the quantitative analysis of BAs in rat serum.

Mentions: In order to find characteristic biomarkers for liver injury induced by HSW, PLS-DA was applied to investigate deep differences between normal control group and raw HSW overdose treatment group, processed HSW overdose treatment group, chlorpromazine treatment group, respectively. The variable influence on the projection (VIP) parameter was applied to select variables that have significant contribution in clusters between two groups, the PLS-DA plots were shown in Figure 3, the loading plots were shown in Figure 4 and variables with VIP > 1 were marked with blue triangles, and the results of model validation plots were shown in Figure S3 depicted in Supplementary Material. The PLS-DA parameters including R2X, R2Y, Q2Y, R2-intercept and Q2-intercept were listed in Table S6. The PLS-DA clusters between two groups showed that normal control group and raw HSW treatment group could be distinguished with BAs in bile and also in serum. Normal control group and chlorpromazine treatment group could be distinguished with BAs in bile and also in serum. There was cross between normal control group and processed HSW treatment with BAs in bile and also in serum. The variables with VIP > 1 in clusters between normal control group and raw HSW treatment group were selected as potential biomarkers, as there was no obvious liver injury in process HSW treatment group, the variables with VIP > 1 in clusters between normal control group and process HSW treatment group were excluded, as a result GDCA in bile, as well as HDCA in serum were selected as potential biomarkers for HSW induced liver injury.


Screening for biomarkers of liver injury induced by Polygonum multiflorum: a targeted metabolomic study.

Dong Q, Li N, Li Q, Zhang CE, Feng WW, Li GQ, Li RY, Tu C, Han X, Bai ZF, Zhang YM, Niu M, Ma ZJ, Xiao XH, Wang JB - Front Pharmacol (2015)

Discrimination of normal control rats from raw HSW, processed HSW and chlorpromazine treatment rats according to PLS-DA. (A–C) Discriminations based on the quantitative analysis of BAs in rat bile. (D–F) Discriminations based on the quantitative analysis of BAs in rat serum.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591842&req=5

Figure 3: Discrimination of normal control rats from raw HSW, processed HSW and chlorpromazine treatment rats according to PLS-DA. (A–C) Discriminations based on the quantitative analysis of BAs in rat bile. (D–F) Discriminations based on the quantitative analysis of BAs in rat serum.
Mentions: In order to find characteristic biomarkers for liver injury induced by HSW, PLS-DA was applied to investigate deep differences between normal control group and raw HSW overdose treatment group, processed HSW overdose treatment group, chlorpromazine treatment group, respectively. The variable influence on the projection (VIP) parameter was applied to select variables that have significant contribution in clusters between two groups, the PLS-DA plots were shown in Figure 3, the loading plots were shown in Figure 4 and variables with VIP > 1 were marked with blue triangles, and the results of model validation plots were shown in Figure S3 depicted in Supplementary Material. The PLS-DA parameters including R2X, R2Y, Q2Y, R2-intercept and Q2-intercept were listed in Table S6. The PLS-DA clusters between two groups showed that normal control group and raw HSW treatment group could be distinguished with BAs in bile and also in serum. Normal control group and chlorpromazine treatment group could be distinguished with BAs in bile and also in serum. There was cross between normal control group and processed HSW treatment with BAs in bile and also in serum. The variables with VIP > 1 in clusters between normal control group and raw HSW treatment group were selected as potential biomarkers, as there was no obvious liver injury in process HSW treatment group, the variables with VIP > 1 in clusters between normal control group and process HSW treatment group were excluded, as a result GDCA in bile, as well as HDCA in serum were selected as potential biomarkers for HSW induced liver injury.

Bottom Line: Heshouwu (HSW), the dry roots of Polygonum multiflorum, a classical traditional Chinese medicine is used as a tonic for a wide range of conditions, particularly those associated with aging.Nine BAs including cholic acid (CA) and chenodeoxycholic acid (CDCA), taurocholic acid (TCA), glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), deoxycholic acid (DCA), glycodeoxycholic acid (GDCA), ursodeoxycholic acid (UDCA), and hyodeoxycholic acid (HDCA) in rat bile and serum were detected by a developed LC-MS method after 42 days treatment.Partial least square-discriminate analysis (PLS-DA) was applied to evaluate the indicative roles of the nine BAs, and metabolism of the nine BAs was summarized.

View Article: PubMed Central - PubMed

Affiliation: China Military Institute of Chinese Medicine, 302 Military Hospital Beijing, China ; College of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu, China.

ABSTRACT
Heshouwu (HSW), the dry roots of Polygonum multiflorum, a classical traditional Chinese medicine is used as a tonic for a wide range of conditions, particularly those associated with aging. However, it tends to be taken overdose or long term in these years, which has resulted in liver damage reported in many countries. In this study, the indicative roles of nine bile acids (BAs) were evaluated to offer potential biomarkers for HSW induced liver injury. Nine BAs including cholic acid (CA) and chenodeoxycholic acid (CDCA), taurocholic acid (TCA), glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), deoxycholic acid (DCA), glycodeoxycholic acid (GDCA), ursodeoxycholic acid (UDCA), and hyodeoxycholic acid (HDCA) in rat bile and serum were detected by a developed LC-MS method after 42 days treatment. Partial least square-discriminate analysis (PLS-DA) was applied to evaluate the indicative roles of the nine BAs, and metabolism of the nine BAs was summarized. Significant change was observed for the concentrations of nine BAs in treatment groups compared with normal control; In the PLS-DA plots of nine BAs in bile, normal control and raw HSW groups were separately clustered and could be clearly distinguished, GDCA was selected as the distinguished components for raw HSW overdose treatment group. In the PLS-DA plots of nine BAs in serum, the normal control and raw HSW overdose treatment group were separately clustered and could be clearly distinguished, and HDCA was selected as the distinguished components for raw HSW overdose treatment group. The results indicated the perturbation of nine BAs was associated with HSW induced liver injury; GDCA in bile, as well as HDCA in serum could be selected as potential biomarkers for HSW induced liver injury; it also laid the foundation for the further search on the mechanisms of liver injury induced by HSW.

No MeSH data available.


Related in: MedlinePlus