Limits...
Conserved overlapping gene arrangement, restricted expression, and biochemical activities of DNA polymerase ν (POLN).

Takata K, Tomida J, Reh S, Swanhart LM, Takata M, Hukriede NA, Wood RD - J. Biol. Chem. (2015)

Bottom Line: Consistent with this, injection of POLN-specific morpholino antisense oligonucleotides did not interfere with zebrafish embryonic development.These properties are conserved with the human enzyme.Although the physiological function of pol ν remains to be clarified, this study uncovers distinctive aspects of its expression control and evolutionarily conserved properties of this DNA polymerase.

View Article: PubMed Central - PubMed

Affiliation: From the Department of Epigenetics and Molecular Carcinogenesis, University of Texas M.D. Anderson Cancer Center, Smithville, Texas 78957, the University of Texas Graduate School of Biomedical Sciences at Houston, Houston, Texas 77030, ktakata@mdanderson.org.

Show MeSH

Related in: MedlinePlus

HAUS3 but not POLN expression can be detected by in situ hybridization in zebrafish.A, real time PCR analysis during zebrafish development. The y axis indicates transcripts per 40 ng of total RNA isolated from pre-mid-blastula transition (Pre-MBT) stage, 10-somite stage, 24-h post-fertilization (hpf) stage, and 48, 72, and 96-hpf, 5-day post-fertilization stage, brain, liver, testis, and oocyte (B). POLN expression in D. rerio is shown. Northern blot analysis in different adult tissues is shown. The same membrane was hybridized, stripped, and rehybridized sequentially with D. rerio POLN, HAUS3, or β-actin probes. C, embryonic expression patterns of zebrafish POLN and HAUS3 mRNA. Panels a–h, HAUS3 in situ hybridization; panels a, b, e, and g are with the antisense probe, and panels c, d, f, and h are with the sense probe. Panels i–p, POLN in situ hybridization. Panels i, j, m, and o are with the antisense probe, and panels k, l, n, and p are with the sense probe. Stages: panels a, c, i, and k are 10 somites; panels b, d, j, and l are 18 somites; panels e, f, m, and n are 24 hpf; panels g, h, o, and p are 48 hpf.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4591814&req=5

Figure 4: HAUS3 but not POLN expression can be detected by in situ hybridization in zebrafish.A, real time PCR analysis during zebrafish development. The y axis indicates transcripts per 40 ng of total RNA isolated from pre-mid-blastula transition (Pre-MBT) stage, 10-somite stage, 24-h post-fertilization (hpf) stage, and 48, 72, and 96-hpf, 5-day post-fertilization stage, brain, liver, testis, and oocyte (B). POLN expression in D. rerio is shown. Northern blot analysis in different adult tissues is shown. The same membrane was hybridized, stripped, and rehybridized sequentially with D. rerio POLN, HAUS3, or β-actin probes. C, embryonic expression patterns of zebrafish POLN and HAUS3 mRNA. Panels a–h, HAUS3 in situ hybridization; panels a, b, e, and g are with the antisense probe, and panels c, d, f, and h are with the sense probe. Panels i–p, POLN in situ hybridization. Panels i, j, m, and o are with the antisense probe, and panels k, l, n, and p are with the sense probe. Stages: panels a, c, i, and k are 10 somites; panels b, d, j, and l are 18 somites; panels e, f, m, and n are 24 hpf; panels g, h, o, and p are 48 hpf.

Mentions: In human cell lines, the pattern of histone H3K27 acetylation, H3K4 trimethylation, and a CpG island suggests a single promoter upstream of the shared first exon (ENCODE) (Fig. 2C) (34). However, the expression patterns of POLN and HAUS3 are quite different, which can be explained by control at the level of alternative splicing of the primary transcript. In zebrafish, POLN is preferentially expressed in testis (Fig. 4A). In comparison, HAUS3 and POLQ are expressed during embryonic development and in several tissues examined (Fig. 4, A and B). Consistent with this, HAUS3 and POLQ are also broadly expressed in human tissues (Proteomic DB).


Conserved overlapping gene arrangement, restricted expression, and biochemical activities of DNA polymerase ν (POLN).

Takata K, Tomida J, Reh S, Swanhart LM, Takata M, Hukriede NA, Wood RD - J. Biol. Chem. (2015)

HAUS3 but not POLN expression can be detected by in situ hybridization in zebrafish.A, real time PCR analysis during zebrafish development. The y axis indicates transcripts per 40 ng of total RNA isolated from pre-mid-blastula transition (Pre-MBT) stage, 10-somite stage, 24-h post-fertilization (hpf) stage, and 48, 72, and 96-hpf, 5-day post-fertilization stage, brain, liver, testis, and oocyte (B). POLN expression in D. rerio is shown. Northern blot analysis in different adult tissues is shown. The same membrane was hybridized, stripped, and rehybridized sequentially with D. rerio POLN, HAUS3, or β-actin probes. C, embryonic expression patterns of zebrafish POLN and HAUS3 mRNA. Panels a–h, HAUS3 in situ hybridization; panels a, b, e, and g are with the antisense probe, and panels c, d, f, and h are with the sense probe. Panels i–p, POLN in situ hybridization. Panels i, j, m, and o are with the antisense probe, and panels k, l, n, and p are with the sense probe. Stages: panels a, c, i, and k are 10 somites; panels b, d, j, and l are 18 somites; panels e, f, m, and n are 24 hpf; panels g, h, o, and p are 48 hpf.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591814&req=5

Figure 4: HAUS3 but not POLN expression can be detected by in situ hybridization in zebrafish.A, real time PCR analysis during zebrafish development. The y axis indicates transcripts per 40 ng of total RNA isolated from pre-mid-blastula transition (Pre-MBT) stage, 10-somite stage, 24-h post-fertilization (hpf) stage, and 48, 72, and 96-hpf, 5-day post-fertilization stage, brain, liver, testis, and oocyte (B). POLN expression in D. rerio is shown. Northern blot analysis in different adult tissues is shown. The same membrane was hybridized, stripped, and rehybridized sequentially with D. rerio POLN, HAUS3, or β-actin probes. C, embryonic expression patterns of zebrafish POLN and HAUS3 mRNA. Panels a–h, HAUS3 in situ hybridization; panels a, b, e, and g are with the antisense probe, and panels c, d, f, and h are with the sense probe. Panels i–p, POLN in situ hybridization. Panels i, j, m, and o are with the antisense probe, and panels k, l, n, and p are with the sense probe. Stages: panels a, c, i, and k are 10 somites; panels b, d, j, and l are 18 somites; panels e, f, m, and n are 24 hpf; panels g, h, o, and p are 48 hpf.
Mentions: In human cell lines, the pattern of histone H3K27 acetylation, H3K4 trimethylation, and a CpG island suggests a single promoter upstream of the shared first exon (ENCODE) (Fig. 2C) (34). However, the expression patterns of POLN and HAUS3 are quite different, which can be explained by control at the level of alternative splicing of the primary transcript. In zebrafish, POLN is preferentially expressed in testis (Fig. 4A). In comparison, HAUS3 and POLQ are expressed during embryonic development and in several tissues examined (Fig. 4, A and B). Consistent with this, HAUS3 and POLQ are also broadly expressed in human tissues (Proteomic DB).

Bottom Line: Consistent with this, injection of POLN-specific morpholino antisense oligonucleotides did not interfere with zebrafish embryonic development.These properties are conserved with the human enzyme.Although the physiological function of pol ν remains to be clarified, this study uncovers distinctive aspects of its expression control and evolutionarily conserved properties of this DNA polymerase.

View Article: PubMed Central - PubMed

Affiliation: From the Department of Epigenetics and Molecular Carcinogenesis, University of Texas M.D. Anderson Cancer Center, Smithville, Texas 78957, the University of Texas Graduate School of Biomedical Sciences at Houston, Houston, Texas 77030, ktakata@mdanderson.org.

Show MeSH
Related in: MedlinePlus