Conserved overlapping gene arrangement, restricted expression, and biochemical activities of DNA polymerase ν (POLN).
Bottom Line: Consistent with this, injection of POLN-specific morpholino antisense oligonucleotides did not interfere with zebrafish embryonic development.These properties are conserved with the human enzyme.Although the physiological function of pol ν remains to be clarified, this study uncovers distinctive aspects of its expression control and evolutionarily conserved properties of this DNA polymerase.
Affiliation: From the Department of Epigenetics and Molecular Carcinogenesis, University of Texas M.D. Anderson Cancer Center, Smithville, Texas 78957, the University of Texas Graduate School of Biomedical Sciences at Houston, Houston, Texas 77030, email@example.com.Show MeSH
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Mentions: Exons encoding the HAUS3 protein are located just upstream of and closely adjacent to POLN (Fig. 2). HAUS3 encodes subunit 3 of the multisubunit augmin protein complex, critical for regulation of centrosome and spindle integrity. This syntenic relationship appears to be conserved in vertebrates. Surprisingly, our searches of transcript databases revealed that the zebrafish POLN transcript overlaps with the 5′UTR of HAUS3, indicating that both share the first exon (Fig. 2A). To investigate this further, we used RACE-PCR to determine the 5′UTR sequence of human POLN. All of the 5′UTR sequence clones for HsPOLN identified in this analysis overlapped with the 5′UTR of HsHAUS3 (Fig. 3A). Similarly, the 5′UTR of the Poln transcript isolated from mouse testis RNA (2) overlaps with the 5′UTR sequence of MmHaus3 (Fig. 3B). Thus for zebrafish, human, and mouse POLN, experimental data show that the coding exons for another gene (HAUS3) reside within the first intron (Fig. 2B), a unique arrangement among DNA polymerases.
Affiliation: From the Department of Epigenetics and Molecular Carcinogenesis, University of Texas M.D. Anderson Cancer Center, Smithville, Texas 78957, the University of Texas Graduate School of Biomedical Sciences at Houston, Houston, Texas 77030, firstname.lastname@example.org.