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Modulator of apoptosis 1 (MOAP-1) is a tumor suppressor protein linked to the RASSF1A protein.

Law J, Salla M, Zare A, Wong Y, Luong L, Volodko N, Svystun O, Flood K, Lim J, Sung M, Dyck JR, Tan CT, Su YC, Yu VC, Mackey J, Baksh S - J. Biol. Chem. (2015)

Bottom Line: It is an integral partner to the tumor suppressor protein, Ras association domain family 1A (RASSF1A), and functions to activate the Bcl-2 family pro-apoptotic protein Bax.Although RASSF1A is now considered a bona fide tumor suppressor protein, the role of MOAP-1 as a tumor suppressor protein has yet to be determined.Overexpression of MOAP-1 in several cancer cell lines resulted in reduced tumorigenesis and up-regulation of genes involved in cancer regulatory pathways that include apoptosis (p53, Fas, and MST1), DNA damage control (poly(ADP)-ribose polymerase and ataxia telangiectasia mutated), those within the cell metabolism (IR-α, IR-β, and AMP-activated protein kinase), and a stabilizing effect on microtubules.

View Article: PubMed Central - PubMed

Affiliation: From the Departments of Biochemistry and.

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MOAP-1 can inhibit cell proliferation in vitro.A and B, MOAP-1 suppresses H1299 cell growth in culture. H1299 cells stably expressing vector or Myc-MOAP-1 (A) or MOAP-1 shRNA (B) were seeded at 4000 cells per well in a 96-well plate, and an MTT assay was carried out as outlined under “Experimental Procedures.” Inset represents immunoblot to confirm the expression of Myc-MOAP-1 with ERK1/2 as a loading control. A, p value = 0.0006, n = 16; B, p value = 0.0022, n = 8. B, right side, immunoblot to confirm shRNA knockdown of endogenous MOAP-1. IB, immunoblot. C, colony formation was carried out in H1299 stable cells expressing the indicated constructs. p value = 0.0361 and n = 4 −5 for both. H1299 cells were seeded at 600 cells per plate and allowed to grow for 11 days prior to staining with crystal violet. Number of cell colonies were counted by visual examination. The expression of Myc-MOAP-1 can be detected in these cells for >30 days after stables are formed (see Fig. 7C for stable expression of MOAP-1 in H1299).
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Figure 5: MOAP-1 can inhibit cell proliferation in vitro.A and B, MOAP-1 suppresses H1299 cell growth in culture. H1299 cells stably expressing vector or Myc-MOAP-1 (A) or MOAP-1 shRNA (B) were seeded at 4000 cells per well in a 96-well plate, and an MTT assay was carried out as outlined under “Experimental Procedures.” Inset represents immunoblot to confirm the expression of Myc-MOAP-1 with ERK1/2 as a loading control. A, p value = 0.0006, n = 16; B, p value = 0.0022, n = 8. B, right side, immunoblot to confirm shRNA knockdown of endogenous MOAP-1. IB, immunoblot. C, colony formation was carried out in H1299 stable cells expressing the indicated constructs. p value = 0.0361 and n = 4 −5 for both. H1299 cells were seeded at 600 cells per plate and allowed to grow for 11 days prior to staining with crystal violet. Number of cell colonies were counted by visual examination. The expression of Myc-MOAP-1 can be detected in these cells for >30 days after stables are formed (see Fig. 7C for stable expression of MOAP-1 in H1299).

Mentions: A fundamental property that is shared by many tumor suppressor proteins is the ability to negatively regulate cell growth and proliferation. H1299 non-small cell lung carcinoma cells stably expressing Myc-MOAP-1 and HCT116 colon cancer cells transiently expressing shRNA to MOAP-1 were generated. Stable expression in H1299 cells was obtained using G418-resistant pools of cells expressing MOAP-1 to avoid effects of clonal variation. Using the MTT colorimetric assay for cell proliferation (24), we observed that cells expressing Myc-MOAP-1 were capable of inhibiting cell proliferation, whereas cells with reduced MOAP-1 expression promoted cell proliferation (Fig. 5, A and B). Colony formation assay also revealed the growth-suppressive function of MOAP-1 (Fig. 5C). This would suggest importance in inhibiting tumor formation.


Modulator of apoptosis 1 (MOAP-1) is a tumor suppressor protein linked to the RASSF1A protein.

Law J, Salla M, Zare A, Wong Y, Luong L, Volodko N, Svystun O, Flood K, Lim J, Sung M, Dyck JR, Tan CT, Su YC, Yu VC, Mackey J, Baksh S - J. Biol. Chem. (2015)

MOAP-1 can inhibit cell proliferation in vitro.A and B, MOAP-1 suppresses H1299 cell growth in culture. H1299 cells stably expressing vector or Myc-MOAP-1 (A) or MOAP-1 shRNA (B) were seeded at 4000 cells per well in a 96-well plate, and an MTT assay was carried out as outlined under “Experimental Procedures.” Inset represents immunoblot to confirm the expression of Myc-MOAP-1 with ERK1/2 as a loading control. A, p value = 0.0006, n = 16; B, p value = 0.0022, n = 8. B, right side, immunoblot to confirm shRNA knockdown of endogenous MOAP-1. IB, immunoblot. C, colony formation was carried out in H1299 stable cells expressing the indicated constructs. p value = 0.0361 and n = 4 −5 for both. H1299 cells were seeded at 600 cells per plate and allowed to grow for 11 days prior to staining with crystal violet. Number of cell colonies were counted by visual examination. The expression of Myc-MOAP-1 can be detected in these cells for >30 days after stables are formed (see Fig. 7C for stable expression of MOAP-1 in H1299).
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4591801&req=5

Figure 5: MOAP-1 can inhibit cell proliferation in vitro.A and B, MOAP-1 suppresses H1299 cell growth in culture. H1299 cells stably expressing vector or Myc-MOAP-1 (A) or MOAP-1 shRNA (B) were seeded at 4000 cells per well in a 96-well plate, and an MTT assay was carried out as outlined under “Experimental Procedures.” Inset represents immunoblot to confirm the expression of Myc-MOAP-1 with ERK1/2 as a loading control. A, p value = 0.0006, n = 16; B, p value = 0.0022, n = 8. B, right side, immunoblot to confirm shRNA knockdown of endogenous MOAP-1. IB, immunoblot. C, colony formation was carried out in H1299 stable cells expressing the indicated constructs. p value = 0.0361 and n = 4 −5 for both. H1299 cells were seeded at 600 cells per plate and allowed to grow for 11 days prior to staining with crystal violet. Number of cell colonies were counted by visual examination. The expression of Myc-MOAP-1 can be detected in these cells for >30 days after stables are formed (see Fig. 7C for stable expression of MOAP-1 in H1299).
Mentions: A fundamental property that is shared by many tumor suppressor proteins is the ability to negatively regulate cell growth and proliferation. H1299 non-small cell lung carcinoma cells stably expressing Myc-MOAP-1 and HCT116 colon cancer cells transiently expressing shRNA to MOAP-1 were generated. Stable expression in H1299 cells was obtained using G418-resistant pools of cells expressing MOAP-1 to avoid effects of clonal variation. Using the MTT colorimetric assay for cell proliferation (24), we observed that cells expressing Myc-MOAP-1 were capable of inhibiting cell proliferation, whereas cells with reduced MOAP-1 expression promoted cell proliferation (Fig. 5, A and B). Colony formation assay also revealed the growth-suppressive function of MOAP-1 (Fig. 5C). This would suggest importance in inhibiting tumor formation.

Bottom Line: It is an integral partner to the tumor suppressor protein, Ras association domain family 1A (RASSF1A), and functions to activate the Bcl-2 family pro-apoptotic protein Bax.Although RASSF1A is now considered a bona fide tumor suppressor protein, the role of MOAP-1 as a tumor suppressor protein has yet to be determined.Overexpression of MOAP-1 in several cancer cell lines resulted in reduced tumorigenesis and up-regulation of genes involved in cancer regulatory pathways that include apoptosis (p53, Fas, and MST1), DNA damage control (poly(ADP)-ribose polymerase and ataxia telangiectasia mutated), those within the cell metabolism (IR-α, IR-β, and AMP-activated protein kinase), and a stabilizing effect on microtubules.

View Article: PubMed Central - PubMed

Affiliation: From the Departments of Biochemistry and.

Show MeSH
Related in: MedlinePlus