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Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats.

Ahmed RR, Mahmoud A, Ahmed OM, Metwalli A, Ebaid H - Biol. Res. (2015)

Bottom Line: We previously found that whey protein (WP) was able to regulate wound healing normally in streptozotocin (STZ)-diabetic models.At day 4, the WP-treated diabetic wound was significantly reduced compared to that in the corresponding control.This data provides evidence for the potential impact of WP in the up-regulation of Hsp72 and Krt16 in T1D, resulting in an improved wound healing process in diabetic models.

View Article: PubMed Central - PubMed

Affiliation: Cell Biology and Histology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt. shorouk2002os@yahoo.com.

ABSTRACT

Background: Impaired wound healing is a complication of diabetes and a serious problem in clinical practice. We previously found that whey protein (WP) was able to regulate wound healing normally in streptozotocin (STZ)-diabetic models. This subsequent study was designed to assess the effect of WP on heat shock protein-72 (Hsp72) and keratin16 (Krt16) expression during wound healing in diabetic rats.

Methods: WP at a dosage of 100 mg/kg of body weight was orally administered daily to wounded normal and STZ-diabetic rats for 8 days.

Results: At day 4, the WP-treated diabetic wound was significantly reduced compared to that in the corresponding control. Diabetic wounded rats developed severe inflammatory infiltration and moderate capillary dilatation and regeneration. Treated rats had mild necrotic formation, moderate infiltration, moderate to severe capillary dilatation and regeneration, in addition to moderate epidermal formation. Hsp72 and Krt16 densities showed low and dense activity in diabetic wounded and diabetic wounded treated groups, respectively. At day 8, WP-treatment of diabetic wounded animals revealed great amelioration with complete recovery and closure of the wound. Reactivity of Hsp72 and Krt16 was reversed, showing dense and low, or medium and low, activity in the diabetic wounded and diabetic wounded treated groups, respectively. Hsp72 expression in the pancreas was found to show dense reactivity with WP-treated diabetic wound rats.

Conclusion: This data provides evidence for the potential impact of WP in the up-regulation of Hsp72 and Krt16 in T1D, resulting in an improved wound healing process in diabetic models.

No MeSH data available.


Related in: MedlinePlus

Histological changes during the wound-healing process on the first (left), fourth (centre) and eighth (right) days after incision in control wounded (a–c), control wounded treated (d–f), diabetic wounded control (g–i) and diabetic wounded treated (j–l) groups; scab (s), granulation (g), dermis (d), wound (W), keratinization (K), epidermis (p), necrosis (n) and inflammatory cells (m). ×100
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Fig2: Histological changes during the wound-healing process on the first (left), fourth (centre) and eighth (right) days after incision in control wounded (a–c), control wounded treated (d–f), diabetic wounded control (g–i) and diabetic wounded treated (j–l) groups; scab (s), granulation (g), dermis (d), wound (W), keratinization (K), epidermis (p), necrosis (n) and inflammatory cells (m). ×100

Mentions: The histopathological findings of skin are shown in Figs. 2 and 3.Fig. 2


Up-regulation of Hsp72 and keratin16 mediates wound healing in streptozotocin diabetic rats.

Ahmed RR, Mahmoud A, Ahmed OM, Metwalli A, Ebaid H - Biol. Res. (2015)

Histological changes during the wound-healing process on the first (left), fourth (centre) and eighth (right) days after incision in control wounded (a–c), control wounded treated (d–f), diabetic wounded control (g–i) and diabetic wounded treated (j–l) groups; scab (s), granulation (g), dermis (d), wound (W), keratinization (K), epidermis (p), necrosis (n) and inflammatory cells (m). ×100
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4591711&req=5

Fig2: Histological changes during the wound-healing process on the first (left), fourth (centre) and eighth (right) days after incision in control wounded (a–c), control wounded treated (d–f), diabetic wounded control (g–i) and diabetic wounded treated (j–l) groups; scab (s), granulation (g), dermis (d), wound (W), keratinization (K), epidermis (p), necrosis (n) and inflammatory cells (m). ×100
Mentions: The histopathological findings of skin are shown in Figs. 2 and 3.Fig. 2

Bottom Line: We previously found that whey protein (WP) was able to regulate wound healing normally in streptozotocin (STZ)-diabetic models.At day 4, the WP-treated diabetic wound was significantly reduced compared to that in the corresponding control.This data provides evidence for the potential impact of WP in the up-regulation of Hsp72 and Krt16 in T1D, resulting in an improved wound healing process in diabetic models.

View Article: PubMed Central - PubMed

Affiliation: Cell Biology and Histology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt. shorouk2002os@yahoo.com.

ABSTRACT

Background: Impaired wound healing is a complication of diabetes and a serious problem in clinical practice. We previously found that whey protein (WP) was able to regulate wound healing normally in streptozotocin (STZ)-diabetic models. This subsequent study was designed to assess the effect of WP on heat shock protein-72 (Hsp72) and keratin16 (Krt16) expression during wound healing in diabetic rats.

Methods: WP at a dosage of 100 mg/kg of body weight was orally administered daily to wounded normal and STZ-diabetic rats for 8 days.

Results: At day 4, the WP-treated diabetic wound was significantly reduced compared to that in the corresponding control. Diabetic wounded rats developed severe inflammatory infiltration and moderate capillary dilatation and regeneration. Treated rats had mild necrotic formation, moderate infiltration, moderate to severe capillary dilatation and regeneration, in addition to moderate epidermal formation. Hsp72 and Krt16 densities showed low and dense activity in diabetic wounded and diabetic wounded treated groups, respectively. At day 8, WP-treatment of diabetic wounded animals revealed great amelioration with complete recovery and closure of the wound. Reactivity of Hsp72 and Krt16 was reversed, showing dense and low, or medium and low, activity in the diabetic wounded and diabetic wounded treated groups, respectively. Hsp72 expression in the pancreas was found to show dense reactivity with WP-treated diabetic wound rats.

Conclusion: This data provides evidence for the potential impact of WP in the up-regulation of Hsp72 and Krt16 in T1D, resulting in an improved wound healing process in diabetic models.

No MeSH data available.


Related in: MedlinePlus