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A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes.

Hopper DW, Ghan R, Cramer GR - Hortic Res (2014)

Bottom Line: Shiraz, V. vinifera cv.Grenache and V. vinifera cv.Differences in stomatal response to ABA were also detected.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Nevada , Reno, NV 89557, USA.

ABSTRACT
A simple and reliable way of phenotyping plant responses to dehydration was developed. Fully-developed leaves were detached and placed in a closed plastic box containing a salt solution to control the atmospheric water potential in the container. Three hours of dehydration (weight loss of the leaf) was optimal for measuring changes in stomatal response to dehydration. Application of the plant hormone abscisic acid (ABA) prior to leaf detachment decreased the amount of water loss, indicating that the assay was able to detect differences based on a stomatal response to dehydration. Five different Vitis genotypes (V. riparia, V. champinii, V. vinifera cv. Shiraz, V. vinifera cv. Grenache and V. vinifera cv. Cabernet Sauvignon) with known differences in drought tolerance were screened for their dehydration response and the results obtained corresponded to previous reports of stomatal responses in the vineyard. Significant differences in stomatal density along with differences in the amount and rate of water lost indicate differences in dehydration sensitivity among the genotypes screened. Differences in stomatal response to ABA were also detected. Shiraz had the lowest stomatal density and the highest ABA sensitivity among the genotypes screened, yet Shiraz lost the most amount of water, indicating that it was the least sensitive to dehydration. Despite having the highest stomatal density and intermediate stomatal sensitivity to ABA, V. riparia lost the smallest amount of water, indicating that it was the most sensitive to dehydration. The assay presented here represents a simple and reliable phenotyping method for plant responses to leaf dehydration.

No MeSH data available.


Related in: MedlinePlus

(a) Water loss in the dehydration assay expressed on a per leaf basis. Each data point is presented as the mean±s.e.m. (n=18 for Ramsey, Grenache and Shiraz; n=19 for Riparia Gloire and Cabernet Sauvignon). (b) Water loss in the dehydration assay normalized on a leaf weight basis. Each data point is presented as the mean±s.e.m. (n=18 for Ramsey, Grenache and Shiraz; n=19 for Riparia Gloire and Cabernet Sauvignon). (c) Rate of water loss from each genotype in the dehydration assay. Each data point is presented as the mean±s.e.m. (n=14 for Ramsey; n=18 for Grenache and Shiraz; n=19 for Riparia Gloire and Cabernet Sauvignon).
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fig5: (a) Water loss in the dehydration assay expressed on a per leaf basis. Each data point is presented as the mean±s.e.m. (n=18 for Ramsey, Grenache and Shiraz; n=19 for Riparia Gloire and Cabernet Sauvignon). (b) Water loss in the dehydration assay normalized on a leaf weight basis. Each data point is presented as the mean±s.e.m. (n=18 for Ramsey, Grenache and Shiraz; n=19 for Riparia Gloire and Cabernet Sauvignon). (c) Rate of water loss from each genotype in the dehydration assay. Each data point is presented as the mean±s.e.m. (n=14 for Ramsey; n=18 for Grenache and Shiraz; n=19 for Riparia Gloire and Cabernet Sauvignon).

Mentions: Based on the measurements determined above, fully developed leaves were selected and used in the dehydration assay to observe differences among the genotypes based on their dehydration response (Figure 5). On a per leaf basis, Ramsey lost the smallest amount of water and Shiraz lost the most water (Figure 5a). Results from ANOVA indicated highly significant effects for time (p≤0.0001), genotype (p≤0.05) and their interaction (p≤0.0001) (Supplementary data for Fig. 2). These results were consistent with the difference in leaf size between these two genotypes. Leaves of Cabernet Sauvignon, Grenache and Riparia Gloire had similar amounts of water loss per leaf despite having large differences in average leaf size.


A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes.

Hopper DW, Ghan R, Cramer GR - Hortic Res (2014)

(a) Water loss in the dehydration assay expressed on a per leaf basis. Each data point is presented as the mean±s.e.m. (n=18 for Ramsey, Grenache and Shiraz; n=19 for Riparia Gloire and Cabernet Sauvignon). (b) Water loss in the dehydration assay normalized on a leaf weight basis. Each data point is presented as the mean±s.e.m. (n=18 for Ramsey, Grenache and Shiraz; n=19 for Riparia Gloire and Cabernet Sauvignon). (c) Rate of water loss from each genotype in the dehydration assay. Each data point is presented as the mean±s.e.m. (n=14 for Ramsey; n=18 for Grenache and Shiraz; n=19 for Riparia Gloire and Cabernet Sauvignon).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591676&req=5

fig5: (a) Water loss in the dehydration assay expressed on a per leaf basis. Each data point is presented as the mean±s.e.m. (n=18 for Ramsey, Grenache and Shiraz; n=19 for Riparia Gloire and Cabernet Sauvignon). (b) Water loss in the dehydration assay normalized on a leaf weight basis. Each data point is presented as the mean±s.e.m. (n=18 for Ramsey, Grenache and Shiraz; n=19 for Riparia Gloire and Cabernet Sauvignon). (c) Rate of water loss from each genotype in the dehydration assay. Each data point is presented as the mean±s.e.m. (n=14 for Ramsey; n=18 for Grenache and Shiraz; n=19 for Riparia Gloire and Cabernet Sauvignon).
Mentions: Based on the measurements determined above, fully developed leaves were selected and used in the dehydration assay to observe differences among the genotypes based on their dehydration response (Figure 5). On a per leaf basis, Ramsey lost the smallest amount of water and Shiraz lost the most water (Figure 5a). Results from ANOVA indicated highly significant effects for time (p≤0.0001), genotype (p≤0.05) and their interaction (p≤0.0001) (Supplementary data for Fig. 2). These results were consistent with the difference in leaf size between these two genotypes. Leaves of Cabernet Sauvignon, Grenache and Riparia Gloire had similar amounts of water loss per leaf despite having large differences in average leaf size.

Bottom Line: Shiraz, V. vinifera cv.Grenache and V. vinifera cv.Differences in stomatal response to ABA were also detected.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Nevada , Reno, NV 89557, USA.

ABSTRACT
A simple and reliable way of phenotyping plant responses to dehydration was developed. Fully-developed leaves were detached and placed in a closed plastic box containing a salt solution to control the atmospheric water potential in the container. Three hours of dehydration (weight loss of the leaf) was optimal for measuring changes in stomatal response to dehydration. Application of the plant hormone abscisic acid (ABA) prior to leaf detachment decreased the amount of water loss, indicating that the assay was able to detect differences based on a stomatal response to dehydration. Five different Vitis genotypes (V. riparia, V. champinii, V. vinifera cv. Shiraz, V. vinifera cv. Grenache and V. vinifera cv. Cabernet Sauvignon) with known differences in drought tolerance were screened for their dehydration response and the results obtained corresponded to previous reports of stomatal responses in the vineyard. Significant differences in stomatal density along with differences in the amount and rate of water lost indicate differences in dehydration sensitivity among the genotypes screened. Differences in stomatal response to ABA were also detected. Shiraz had the lowest stomatal density and the highest ABA sensitivity among the genotypes screened, yet Shiraz lost the most amount of water, indicating that it was the least sensitive to dehydration. Despite having the highest stomatal density and intermediate stomatal sensitivity to ABA, V. riparia lost the smallest amount of water, indicating that it was the most sensitive to dehydration. The assay presented here represents a simple and reliable phenotyping method for plant responses to leaf dehydration.

No MeSH data available.


Related in: MedlinePlus