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A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes.

Hopper DW, Ghan R, Cramer GR - Hortic Res (2014)

Bottom Line: Shiraz, V. vinifera cv.Grenache and V. vinifera cv.Differences in stomatal response to ABA were also detected.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Nevada , Reno, NV 89557, USA.

ABSTRACT
A simple and reliable way of phenotyping plant responses to dehydration was developed. Fully-developed leaves were detached and placed in a closed plastic box containing a salt solution to control the atmospheric water potential in the container. Three hours of dehydration (weight loss of the leaf) was optimal for measuring changes in stomatal response to dehydration. Application of the plant hormone abscisic acid (ABA) prior to leaf detachment decreased the amount of water loss, indicating that the assay was able to detect differences based on a stomatal response to dehydration. Five different Vitis genotypes (V. riparia, V. champinii, V. vinifera cv. Shiraz, V. vinifera cv. Grenache and V. vinifera cv. Cabernet Sauvignon) with known differences in drought tolerance were screened for their dehydration response and the results obtained corresponded to previous reports of stomatal responses in the vineyard. Significant differences in stomatal density along with differences in the amount and rate of water lost indicate differences in dehydration sensitivity among the genotypes screened. Differences in stomatal response to ABA were also detected. Shiraz had the lowest stomatal density and the highest ABA sensitivity among the genotypes screened, yet Shiraz lost the most amount of water, indicating that it was the least sensitive to dehydration. Despite having the highest stomatal density and intermediate stomatal sensitivity to ABA, V. riparia lost the smallest amount of water, indicating that it was the most sensitive to dehydration. The assay presented here represents a simple and reliable phenotyping method for plant responses to leaf dehydration.

No MeSH data available.


Related in: MedlinePlus

Determination of fully developed leaves along the shoots of five grapevine genotypes. Each data point is presented as the mean±s.e.m. (n=8 for Grenache; n=4 for Shiraz; n=6 for Ramsey, Riparia Gloire and Cabernet Sauvignon).
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fig4: Determination of fully developed leaves along the shoots of five grapevine genotypes. Each data point is presented as the mean±s.e.m. (n=8 for Grenache; n=4 for Shiraz; n=6 for Ramsey, Riparia Gloire and Cabernet Sauvignon).

Mentions: It is important to measure leaf responses among multiple genotypes at the same leaf developmental stage. Fully developed leaves are easy to detect because leaf expansion has ceased. In order to determine the node at which fully developed leaves were found, leaf length (the distance from the petiole to the tip of the leaf down the midvein) from the shoot tip towards the base was measured for the five genotypes in this study (Figure 4). This was to ensure that water lost due to dehydration was not based on differences between leaf age for each genotype. Once leaf length reached a plateau and no longer significantly increased, these leaves were considered fully developed. For Riparia Gloire, this plateau occurred at node 8 with an average leaf length of 16.7 cm, while for the other genotypes, this occurred at node 10 with an average length of 8.0, 10.7, 11.1 and 12.2 cm for Ramsey, Cabernet Sauvignon, Grenache, and Shiraz, respectively (Figure 4). Photosynthetic measurements of these leaves confirmed that the selected leaves were photosynthetically optimal (data not shown).


A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes.

Hopper DW, Ghan R, Cramer GR - Hortic Res (2014)

Determination of fully developed leaves along the shoots of five grapevine genotypes. Each data point is presented as the mean±s.e.m. (n=8 for Grenache; n=4 for Shiraz; n=6 for Ramsey, Riparia Gloire and Cabernet Sauvignon).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591676&req=5

fig4: Determination of fully developed leaves along the shoots of five grapevine genotypes. Each data point is presented as the mean±s.e.m. (n=8 for Grenache; n=4 for Shiraz; n=6 for Ramsey, Riparia Gloire and Cabernet Sauvignon).
Mentions: It is important to measure leaf responses among multiple genotypes at the same leaf developmental stage. Fully developed leaves are easy to detect because leaf expansion has ceased. In order to determine the node at which fully developed leaves were found, leaf length (the distance from the petiole to the tip of the leaf down the midvein) from the shoot tip towards the base was measured for the five genotypes in this study (Figure 4). This was to ensure that water lost due to dehydration was not based on differences between leaf age for each genotype. Once leaf length reached a plateau and no longer significantly increased, these leaves were considered fully developed. For Riparia Gloire, this plateau occurred at node 8 with an average leaf length of 16.7 cm, while for the other genotypes, this occurred at node 10 with an average length of 8.0, 10.7, 11.1 and 12.2 cm for Ramsey, Cabernet Sauvignon, Grenache, and Shiraz, respectively (Figure 4). Photosynthetic measurements of these leaves confirmed that the selected leaves were photosynthetically optimal (data not shown).

Bottom Line: Shiraz, V. vinifera cv.Grenache and V. vinifera cv.Differences in stomatal response to ABA were also detected.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Nevada , Reno, NV 89557, USA.

ABSTRACT
A simple and reliable way of phenotyping plant responses to dehydration was developed. Fully-developed leaves were detached and placed in a closed plastic box containing a salt solution to control the atmospheric water potential in the container. Three hours of dehydration (weight loss of the leaf) was optimal for measuring changes in stomatal response to dehydration. Application of the plant hormone abscisic acid (ABA) prior to leaf detachment decreased the amount of water loss, indicating that the assay was able to detect differences based on a stomatal response to dehydration. Five different Vitis genotypes (V. riparia, V. champinii, V. vinifera cv. Shiraz, V. vinifera cv. Grenache and V. vinifera cv. Cabernet Sauvignon) with known differences in drought tolerance were screened for their dehydration response and the results obtained corresponded to previous reports of stomatal responses in the vineyard. Significant differences in stomatal density along with differences in the amount and rate of water lost indicate differences in dehydration sensitivity among the genotypes screened. Differences in stomatal response to ABA were also detected. Shiraz had the lowest stomatal density and the highest ABA sensitivity among the genotypes screened, yet Shiraz lost the most amount of water, indicating that it was the least sensitive to dehydration. Despite having the highest stomatal density and intermediate stomatal sensitivity to ABA, V. riparia lost the smallest amount of water, indicating that it was the most sensitive to dehydration. The assay presented here represents a simple and reliable phenotyping method for plant responses to leaf dehydration.

No MeSH data available.


Related in: MedlinePlus