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A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes.

Hopper DW, Ghan R, Cramer GR - Hortic Res (2014)

Bottom Line: Shiraz, V. vinifera cv.Grenache and V. vinifera cv.Differences in stomatal response to ABA were also detected.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Nevada , Reno, NV 89557, USA.

ABSTRACT
A simple and reliable way of phenotyping plant responses to dehydration was developed. Fully-developed leaves were detached and placed in a closed plastic box containing a salt solution to control the atmospheric water potential in the container. Three hours of dehydration (weight loss of the leaf) was optimal for measuring changes in stomatal response to dehydration. Application of the plant hormone abscisic acid (ABA) prior to leaf detachment decreased the amount of water loss, indicating that the assay was able to detect differences based on a stomatal response to dehydration. Five different Vitis genotypes (V. riparia, V. champinii, V. vinifera cv. Shiraz, V. vinifera cv. Grenache and V. vinifera cv. Cabernet Sauvignon) with known differences in drought tolerance were screened for their dehydration response and the results obtained corresponded to previous reports of stomatal responses in the vineyard. Significant differences in stomatal density along with differences in the amount and rate of water lost indicate differences in dehydration sensitivity among the genotypes screened. Differences in stomatal response to ABA were also detected. Shiraz had the lowest stomatal density and the highest ABA sensitivity among the genotypes screened, yet Shiraz lost the most amount of water, indicating that it was the least sensitive to dehydration. Despite having the highest stomatal density and intermediate stomatal sensitivity to ABA, V. riparia lost the smallest amount of water, indicating that it was the most sensitive to dehydration. The assay presented here represents a simple and reliable phenotyping method for plant responses to leaf dehydration.

No MeSH data available.


Related in: MedlinePlus

The effect of a 4-h treatment of ABA on water loss in the leaf dehydration assay. Each data point is presented as the mean±s.e.m. Control (n=2); 1 μM and 10 μM ABA (n=3).
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fig2: The effect of a 4-h treatment of ABA on water loss in the leaf dehydration assay. Each data point is presented as the mean±s.e.m. Control (n=2); 1 μM and 10 μM ABA (n=3).

Mentions: To test if water loss from the leaves was primarily a response of stomata to dehydration, ABA, a known elicitor of stomatal closure, was applied (Figure 2). Intact leaves were sprayed with different concentrations of ABA. The dehydration assay was performed after 4 h of ABA treatment, when gas exchange measurements indicated that conditions were at steady state. Application of ABA significantly affected the amount of water lost (p≤0.01) (Supplementary Fig. 1). The slope of the first hour of water loss is lower at 10 μM ABA (0.0006) compared to both control (0.0013) and 1 μM ABA (0.0011) (lines not shown). It also decreases significantly the overall amount of water loss in a 3-h period, indicating that water loss is affected by stomatal responses.


A rapid dehydration leaf assay reveals stomatal response differences in grapevine genotypes.

Hopper DW, Ghan R, Cramer GR - Hortic Res (2014)

The effect of a 4-h treatment of ABA on water loss in the leaf dehydration assay. Each data point is presented as the mean±s.e.m. Control (n=2); 1 μM and 10 μM ABA (n=3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591676&req=5

fig2: The effect of a 4-h treatment of ABA on water loss in the leaf dehydration assay. Each data point is presented as the mean±s.e.m. Control (n=2); 1 μM and 10 μM ABA (n=3).
Mentions: To test if water loss from the leaves was primarily a response of stomata to dehydration, ABA, a known elicitor of stomatal closure, was applied (Figure 2). Intact leaves were sprayed with different concentrations of ABA. The dehydration assay was performed after 4 h of ABA treatment, when gas exchange measurements indicated that conditions were at steady state. Application of ABA significantly affected the amount of water lost (p≤0.01) (Supplementary Fig. 1). The slope of the first hour of water loss is lower at 10 μM ABA (0.0006) compared to both control (0.0013) and 1 μM ABA (0.0011) (lines not shown). It also decreases significantly the overall amount of water loss in a 3-h period, indicating that water loss is affected by stomatal responses.

Bottom Line: Shiraz, V. vinifera cv.Grenache and V. vinifera cv.Differences in stomatal response to ABA were also detected.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, University of Nevada , Reno, NV 89557, USA.

ABSTRACT
A simple and reliable way of phenotyping plant responses to dehydration was developed. Fully-developed leaves were detached and placed in a closed plastic box containing a salt solution to control the atmospheric water potential in the container. Three hours of dehydration (weight loss of the leaf) was optimal for measuring changes in stomatal response to dehydration. Application of the plant hormone abscisic acid (ABA) prior to leaf detachment decreased the amount of water loss, indicating that the assay was able to detect differences based on a stomatal response to dehydration. Five different Vitis genotypes (V. riparia, V. champinii, V. vinifera cv. Shiraz, V. vinifera cv. Grenache and V. vinifera cv. Cabernet Sauvignon) with known differences in drought tolerance were screened for their dehydration response and the results obtained corresponded to previous reports of stomatal responses in the vineyard. Significant differences in stomatal density along with differences in the amount and rate of water lost indicate differences in dehydration sensitivity among the genotypes screened. Differences in stomatal response to ABA were also detected. Shiraz had the lowest stomatal density and the highest ABA sensitivity among the genotypes screened, yet Shiraz lost the most amount of water, indicating that it was the least sensitive to dehydration. Despite having the highest stomatal density and intermediate stomatal sensitivity to ABA, V. riparia lost the smallest amount of water, indicating that it was the most sensitive to dehydration. The assay presented here represents a simple and reliable phenotyping method for plant responses to leaf dehydration.

No MeSH data available.


Related in: MedlinePlus