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Aflatoxin B1 Detection Using a Highly-Sensitive Molecularly-Imprinted Electrochemical Sensor Based on an Electropolymerized Metal Organic Framework.

Jiang M, Braiek M, Florea A, Chrouda A, Farre C, Bonhomme A, Bessueille F, Vocanson F, Zhang A, Jaffrezic-Renault N - Toxins (Basel) (2015)

Bottom Line: The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM.Compared to the non-imprinted sensor, the imprinting factor was found to be 10.Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. mjjiang@mails.ccnu.edu.cn.

ABSTRACT
A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

No MeSH data available.


Related in: MedlinePlus

Calibration curves upon analysis of various concentrations of aflatoxins and ochratoxin A. Curve 1: NIP sensor for AFB1; Curve 2: MIP sensor for AFB1; Curve 3: MIP sensor for AFB2; Curve 4: MIP sensor for AFG1; Curve 5: MIP sensor for OTA.
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toxins-07-03540-f010: Calibration curves upon analysis of various concentrations of aflatoxins and ochratoxin A. Curve 1: NIP sensor for AFB1; Curve 2: MIP sensor for AFB1; Curve 3: MIP sensor for AFB2; Curve 4: MIP sensor for AFG1; Curve 5: MIP sensor for OTA.

Mentions: Selectivity is a determining parameter in the analytical features of the MIP-MOF sensor. The responses for mycotoxins of similar structures, such as aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), and ochratoxin A (OTA) have been examined. Figure 10 displays the response for different concentrations of interfering species; the peak current density ratio (signal-blank)/blank versus logarithm of different concentrations exhibit a linear response over a range from 3.2 fM to 3.2 μM. The cross-reactivity in percent of the MIP-MOF sensor for AFB2, AFG1, and OTA is reported in Table 2, as one hundred times the ratio of concentration of AFB1 giving a response of MIP-MOF sensor to the concentration of other mycotoxin giving the same response. The results show that the proposed biosensor has high selectivity for aflatoxin B1.


Aflatoxin B1 Detection Using a Highly-Sensitive Molecularly-Imprinted Electrochemical Sensor Based on an Electropolymerized Metal Organic Framework.

Jiang M, Braiek M, Florea A, Chrouda A, Farre C, Bonhomme A, Bessueille F, Vocanson F, Zhang A, Jaffrezic-Renault N - Toxins (Basel) (2015)

Calibration curves upon analysis of various concentrations of aflatoxins and ochratoxin A. Curve 1: NIP sensor for AFB1; Curve 2: MIP sensor for AFB1; Curve 3: MIP sensor for AFB2; Curve 4: MIP sensor for AFG1; Curve 5: MIP sensor for OTA.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591663&req=5

toxins-07-03540-f010: Calibration curves upon analysis of various concentrations of aflatoxins and ochratoxin A. Curve 1: NIP sensor for AFB1; Curve 2: MIP sensor for AFB1; Curve 3: MIP sensor for AFB2; Curve 4: MIP sensor for AFG1; Curve 5: MIP sensor for OTA.
Mentions: Selectivity is a determining parameter in the analytical features of the MIP-MOF sensor. The responses for mycotoxins of similar structures, such as aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), and ochratoxin A (OTA) have been examined. Figure 10 displays the response for different concentrations of interfering species; the peak current density ratio (signal-blank)/blank versus logarithm of different concentrations exhibit a linear response over a range from 3.2 fM to 3.2 μM. The cross-reactivity in percent of the MIP-MOF sensor for AFB2, AFG1, and OTA is reported in Table 2, as one hundred times the ratio of concentration of AFB1 giving a response of MIP-MOF sensor to the concentration of other mycotoxin giving the same response. The results show that the proposed biosensor has high selectivity for aflatoxin B1.

Bottom Line: The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM.Compared to the non-imprinted sensor, the imprinting factor was found to be 10.Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. mjjiang@mails.ccnu.edu.cn.

ABSTRACT
A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

No MeSH data available.


Related in: MedlinePlus