Limits...
Aflatoxin B1 Detection Using a Highly-Sensitive Molecularly-Imprinted Electrochemical Sensor Based on an Electropolymerized Metal Organic Framework.

Jiang M, Braiek M, Florea A, Chrouda A, Farre C, Bonhomme A, Bessueille F, Vocanson F, Zhang A, Jaffrezic-Renault N - Toxins (Basel) (2015)

Bottom Line: The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM.Compared to the non-imprinted sensor, the imprinting factor was found to be 10.Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. mjjiang@mails.ccnu.edu.cn.

ABSTRACT
A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

No MeSH data available.


Related in: MedlinePlus

Calibration curves obtained for MIP (red curve) and NIP (brown curve) sensors by LSV for 3.2 fM to 32 nM aflatoxin. Under optimized parameters: extraction time 30 min, incubation time 20 min. I = signal after incubation with aflatoxin. IO = signal after extraction.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4591663&req=5

toxins-07-03540-f009: Calibration curves obtained for MIP (red curve) and NIP (brown curve) sensors by LSV for 3.2 fM to 32 nM aflatoxin. Under optimized parameters: extraction time 30 min, incubation time 20 min. I = signal after incubation with aflatoxin. IO = signal after extraction.

Mentions: From Figure 9 we can see the dependence of LSV current density on the concentration of aflatoxin. Data points were calculated as the average of three different electrodes. The peak current density ratio (signal-blank)/blank versus logarithm function of aflatoxin concentration exhibited a linear response over a range from 3.2 fM to 3.2 μM, with a limit of quantification of 3 fM (1 pg/L) and a limit of detection of 1 fM (0.3 pg/L). The limit of quantification (LOQ) was determined to be 10 σb and the limit of detection as 3 σb, σb being the standard deviation of the blank. The low signals obtained for NIP prove the influence of the template in obtaining binding sites for aflatoxin. The imprinting factor (ratio of sensitivity for MIP to sensitivity for NIP) is equal to 11.


Aflatoxin B1 Detection Using a Highly-Sensitive Molecularly-Imprinted Electrochemical Sensor Based on an Electropolymerized Metal Organic Framework.

Jiang M, Braiek M, Florea A, Chrouda A, Farre C, Bonhomme A, Bessueille F, Vocanson F, Zhang A, Jaffrezic-Renault N - Toxins (Basel) (2015)

Calibration curves obtained for MIP (red curve) and NIP (brown curve) sensors by LSV for 3.2 fM to 32 nM aflatoxin. Under optimized parameters: extraction time 30 min, incubation time 20 min. I = signal after incubation with aflatoxin. IO = signal after extraction.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591663&req=5

toxins-07-03540-f009: Calibration curves obtained for MIP (red curve) and NIP (brown curve) sensors by LSV for 3.2 fM to 32 nM aflatoxin. Under optimized parameters: extraction time 30 min, incubation time 20 min. I = signal after incubation with aflatoxin. IO = signal after extraction.
Mentions: From Figure 9 we can see the dependence of LSV current density on the concentration of aflatoxin. Data points were calculated as the average of three different electrodes. The peak current density ratio (signal-blank)/blank versus logarithm function of aflatoxin concentration exhibited a linear response over a range from 3.2 fM to 3.2 μM, with a limit of quantification of 3 fM (1 pg/L) and a limit of detection of 1 fM (0.3 pg/L). The limit of quantification (LOQ) was determined to be 10 σb and the limit of detection as 3 σb, σb being the standard deviation of the blank. The low signals obtained for NIP prove the influence of the template in obtaining binding sites for aflatoxin. The imprinting factor (ratio of sensitivity for MIP to sensitivity for NIP) is equal to 11.

Bottom Line: The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM.Compared to the non-imprinted sensor, the imprinting factor was found to be 10.Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. mjjiang@mails.ccnu.edu.cn.

ABSTRACT
A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

No MeSH data available.


Related in: MedlinePlus