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Aflatoxin B1 Detection Using a Highly-Sensitive Molecularly-Imprinted Electrochemical Sensor Based on an Electropolymerized Metal Organic Framework.

Jiang M, Braiek M, Florea A, Chrouda A, Farre C, Bonhomme A, Bessueille F, Vocanson F, Zhang A, Jaffrezic-Renault N - Toxins (Basel) (2015)

Bottom Line: The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM.Compared to the non-imprinted sensor, the imprinting factor was found to be 10.Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. mjjiang@mails.ccnu.edu.cn.

ABSTRACT
A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

No MeSH data available.


Related in: MedlinePlus

AFM phase image showing gold nanoparticles included in the MIP MOF film. Size of image: 1 µm × 1 µm; Vertical scale: phase from −10.3 degrees to +14.3 degrees.
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toxins-07-03540-f006: AFM phase image showing gold nanoparticles included in the MIP MOF film. Size of image: 1 µm × 1 µm; Vertical scale: phase from −10.3 degrees to +14.3 degrees.

Mentions: Figure 6 shows the phase of the prepared film, observed by AFM, which offers important information about the elasticity modulus of the MIP MOF film surface. This hybrid polymer film presents a very high phase variation: 24 degrees. This variation is due to the apparent AuNPs, their apparent diameter being around 30 nm, which is larger than that measured with TEM, due to the curvature radii of the silicon tip used.


Aflatoxin B1 Detection Using a Highly-Sensitive Molecularly-Imprinted Electrochemical Sensor Based on an Electropolymerized Metal Organic Framework.

Jiang M, Braiek M, Florea A, Chrouda A, Farre C, Bonhomme A, Bessueille F, Vocanson F, Zhang A, Jaffrezic-Renault N - Toxins (Basel) (2015)

AFM phase image showing gold nanoparticles included in the MIP MOF film. Size of image: 1 µm × 1 µm; Vertical scale: phase from −10.3 degrees to +14.3 degrees.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591663&req=5

toxins-07-03540-f006: AFM phase image showing gold nanoparticles included in the MIP MOF film. Size of image: 1 µm × 1 µm; Vertical scale: phase from −10.3 degrees to +14.3 degrees.
Mentions: Figure 6 shows the phase of the prepared film, observed by AFM, which offers important information about the elasticity modulus of the MIP MOF film surface. This hybrid polymer film presents a very high phase variation: 24 degrees. This variation is due to the apparent AuNPs, their apparent diameter being around 30 nm, which is larger than that measured with TEM, due to the curvature radii of the silicon tip used.

Bottom Line: The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM.Compared to the non-imprinted sensor, the imprinting factor was found to be 10.Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. mjjiang@mails.ccnu.edu.cn.

ABSTRACT
A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

No MeSH data available.


Related in: MedlinePlus