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Aflatoxin B1 Detection Using a Highly-Sensitive Molecularly-Imprinted Electrochemical Sensor Based on an Electropolymerized Metal Organic Framework.

Jiang M, Braiek M, Florea A, Chrouda A, Farre C, Bonhomme A, Bessueille F, Vocanson F, Zhang A, Jaffrezic-Renault N - Toxins (Basel) (2015)

Bottom Line: The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM.Compared to the non-imprinted sensor, the imprinting factor was found to be 10.Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. mjjiang@mails.ccnu.edu.cn.

ABSTRACT
A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

No MeSH data available.


Related in: MedlinePlus

Electropolymerization of MIP by CV. Potential range from −0.35 V to 0.8 V vs. SCE. Scan rate 100 mV/s.
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toxins-07-03540-f005: Electropolymerization of MIP by CV. Potential range from −0.35 V to 0.8 V vs. SCE. Scan rate 100 mV/s.

Mentions: The electropolymerizaton was performed by CV in a solution of 10 mM [Fe(CN)6]3−/4− in PBS pH 7.2 and the film formation was examined through the changes in the current per cycle. As shown in Figure 5, the current decreased as the number of cycles increased, suggesting gradual growth of a compact polymeric film which covered the surface of the gold electrode, hindering the charge transfer of the redox probe at the electrode. The electrochemical polymerization process consists of intermolecular reactions between the aniline moieties of PATP.


Aflatoxin B1 Detection Using a Highly-Sensitive Molecularly-Imprinted Electrochemical Sensor Based on an Electropolymerized Metal Organic Framework.

Jiang M, Braiek M, Florea A, Chrouda A, Farre C, Bonhomme A, Bessueille F, Vocanson F, Zhang A, Jaffrezic-Renault N - Toxins (Basel) (2015)

Electropolymerization of MIP by CV. Potential range from −0.35 V to 0.8 V vs. SCE. Scan rate 100 mV/s.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591663&req=5

toxins-07-03540-f005: Electropolymerization of MIP by CV. Potential range from −0.35 V to 0.8 V vs. SCE. Scan rate 100 mV/s.
Mentions: The electropolymerizaton was performed by CV in a solution of 10 mM [Fe(CN)6]3−/4− in PBS pH 7.2 and the film formation was examined through the changes in the current per cycle. As shown in Figure 5, the current decreased as the number of cycles increased, suggesting gradual growth of a compact polymeric film which covered the surface of the gold electrode, hindering the charge transfer of the redox probe at the electrode. The electrochemical polymerization process consists of intermolecular reactions between the aniline moieties of PATP.

Bottom Line: The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM.Compared to the non-imprinted sensor, the imprinting factor was found to be 10.Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. mjjiang@mails.ccnu.edu.cn.

ABSTRACT
A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

No MeSH data available.


Related in: MedlinePlus