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Aflatoxin B1 Detection Using a Highly-Sensitive Molecularly-Imprinted Electrochemical Sensor Based on an Electropolymerized Metal Organic Framework.

Jiang M, Braiek M, Florea A, Chrouda A, Farre C, Bonhomme A, Bessueille F, Vocanson F, Zhang A, Jaffrezic-Renault N - Toxins (Basel) (2015)

Bottom Line: The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM.Compared to the non-imprinted sensor, the imprinting factor was found to be 10.Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. mjjiang@mails.ccnu.edu.cn.

ABSTRACT
A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

No MeSH data available.


Related in: MedlinePlus

FTIR spectrum of p-aminothiophenol (ATP) and of p-aminothiophenol-functionalized gold nanoparticles (Au-ATP).
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toxins-07-03540-f004: FTIR spectrum of p-aminothiophenol (ATP) and of p-aminothiophenol-functionalized gold nanoparticles (Au-ATP).

Mentions: The FTIR spectrum of p-aminothiophenol was compared to that of p-aminothiophenol-functionalized gold nanoparticles (cf Figure 4). The main peaks of p-aminothiophenol are present in both FTIR transmission spectra (1202 cm−1, 1489 cm−1, 1589 cm−1).


Aflatoxin B1 Detection Using a Highly-Sensitive Molecularly-Imprinted Electrochemical Sensor Based on an Electropolymerized Metal Organic Framework.

Jiang M, Braiek M, Florea A, Chrouda A, Farre C, Bonhomme A, Bessueille F, Vocanson F, Zhang A, Jaffrezic-Renault N - Toxins (Basel) (2015)

FTIR spectrum of p-aminothiophenol (ATP) and of p-aminothiophenol-functionalized gold nanoparticles (Au-ATP).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591663&req=5

toxins-07-03540-f004: FTIR spectrum of p-aminothiophenol (ATP) and of p-aminothiophenol-functionalized gold nanoparticles (Au-ATP).
Mentions: The FTIR spectrum of p-aminothiophenol was compared to that of p-aminothiophenol-functionalized gold nanoparticles (cf Figure 4). The main peaks of p-aminothiophenol are present in both FTIR transmission spectra (1202 cm−1, 1489 cm−1, 1589 cm−1).

Bottom Line: The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM.Compared to the non-imprinted sensor, the imprinting factor was found to be 10.Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China. mjjiang@mails.ccnu.edu.cn.

ABSTRACT
A sensitive electrochemical molecularly-imprinted sensor was developed for the detection of aflatoxin B1 (AFB1), by electropolymerization of p-aminothiophenol-functionalized gold nanoparticles in the presence of AFB1 as a template molecule. The extraction of the template leads to the formation of cavities that are able to specifically recognize and bind AFB1 through π-π interactions between AFB1 molecules and aniline moities. The performance of the developed sensor for the detection of AFB1 was investigated by linear sweep voltammetry using a hexacyanoferrate/hexacyanoferrite solution as a redox probe, the electron transfer rate increasing when the concentration of AFB1 increases, due to a p-doping effect. The molecularly-imprinted sensor exhibits a broad linear range, between 3.2 fM and 3.2 µM, and a quantification limit of 3 fM. Compared to the non-imprinted sensor, the imprinting factor was found to be 10. Selectivity studies were also performed towards the binding of other aflatoxins and ochratoxin A, proving good selectivity.

No MeSH data available.


Related in: MedlinePlus