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Staphylococcus epidermidis and Staphylococcus haemolyticus: Molecular Detection of Cytotoxin and Enterotoxin Genes.

Pinheiro L, Brito CI, de Oliveira A, Martins PY, Pereira VC, da Cunha Mde L - Toxins (Basel) (2015)

Bottom Line: The most frequent enterotoxin genes were sea (53.3%), seg (64.5%) and sei (67.5%).The seg gene was positively associated with S. epidermidis (p = 0.02), and this species was more toxigenic than S. haemolyticus.The hla/yidD gene was detected in 92.9% of S. epidermidis and the hla gene in 91.7% of S. haemolyticus isolates; hlb was detected in 92.9% of the S. epidermidis isolates and hld in 95.3%.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiologia e Imunologia, Instituto de Biociências, UNESP-Univ Estadual Paulista, Botucatu, SP 18618-970, Brasil. luizapinheiro@ibb.unesp.br.

ABSTRACT
Although opportunistic pathogens, coagulase-negative staphylococci (CoNS), including Staphylococcus epidermidis and Staphylococcus haemolyticus, have long been regarded as avirulent organisms. The role of toxins in the development of infections caused by CoNS is still controversial. The objective of this study was to characterize the presence of enterotoxin and cytotoxin genes in S. epidermidis and S. haemolyticus isolates obtained from blood cultures. Cytotoxin genes were detected by PCR using novel species-specific primers. Among the 85 S. epidermidis and 84 S. haemolyticus isolates, 95.3% and 79.8%, respectively, carried at least one enterotoxin gene. The most frequent enterotoxin genes were sea (53.3%), seg (64.5%) and sei (67.5%). The seg gene was positively associated with S. epidermidis (p = 0.02), and this species was more toxigenic than S. haemolyticus. The hla/yidD gene was detected in 92.9% of S. epidermidis and the hla gene in 91.7% of S. haemolyticus isolates; hlb was detected in 92.9% of the S. epidermidis isolates and hld in 95.3%. Nosocomial Staphylococcus epidermidis and S. haemolyticus isolates exhibited a high toxigenic potential, mainly producing the non-classical enterotoxins seg and sei. The previously unreported detection of hla/yidD and hlb in S. epidermidis and S. haemolyticus using species-specific primers showed that these hemolysin genes differ between CoNS species and that they are highly frequent in blood culture isolates.

No MeSH data available.


Related in: MedlinePlus

Detection of the enterotoxin genes sea–sei in S. epidermidis and S. haemolyticus isolates. * Significantly positive association with S. epidermidis.
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toxins-07-03688-f001: Detection of the enterotoxin genes sea–sei in S. epidermidis and S. haemolyticus isolates. * Significantly positive association with S. epidermidis.

Mentions: Figure 1 illustrates the detection of enterotoxin genes in the S. haemolyticus and S. epidermidis isolates. The proportion of positive isolates was higher for the latter species, except for seb and seh (34% and 15%, respectively), which were more frequent in S. haemolyticus. The sed and see genes were rarely found (2% and 3%, respectively), while sei, seg and sea were the most frequent genes in both species. Detection of the seg gene was significantly associated with S. epidermidis (p = 0.02).


Staphylococcus epidermidis and Staphylococcus haemolyticus: Molecular Detection of Cytotoxin and Enterotoxin Genes.

Pinheiro L, Brito CI, de Oliveira A, Martins PY, Pereira VC, da Cunha Mde L - Toxins (Basel) (2015)

Detection of the enterotoxin genes sea–sei in S. epidermidis and S. haemolyticus isolates. * Significantly positive association with S. epidermidis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591658&req=5

toxins-07-03688-f001: Detection of the enterotoxin genes sea–sei in S. epidermidis and S. haemolyticus isolates. * Significantly positive association with S. epidermidis.
Mentions: Figure 1 illustrates the detection of enterotoxin genes in the S. haemolyticus and S. epidermidis isolates. The proportion of positive isolates was higher for the latter species, except for seb and seh (34% and 15%, respectively), which were more frequent in S. haemolyticus. The sed and see genes were rarely found (2% and 3%, respectively), while sei, seg and sea were the most frequent genes in both species. Detection of the seg gene was significantly associated with S. epidermidis (p = 0.02).

Bottom Line: The most frequent enterotoxin genes were sea (53.3%), seg (64.5%) and sei (67.5%).The seg gene was positively associated with S. epidermidis (p = 0.02), and this species was more toxigenic than S. haemolyticus.The hla/yidD gene was detected in 92.9% of S. epidermidis and the hla gene in 91.7% of S. haemolyticus isolates; hlb was detected in 92.9% of the S. epidermidis isolates and hld in 95.3%.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Microbiologia e Imunologia, Instituto de Biociências, UNESP-Univ Estadual Paulista, Botucatu, SP 18618-970, Brasil. luizapinheiro@ibb.unesp.br.

ABSTRACT
Although opportunistic pathogens, coagulase-negative staphylococci (CoNS), including Staphylococcus epidermidis and Staphylococcus haemolyticus, have long been regarded as avirulent organisms. The role of toxins in the development of infections caused by CoNS is still controversial. The objective of this study was to characterize the presence of enterotoxin and cytotoxin genes in S. epidermidis and S. haemolyticus isolates obtained from blood cultures. Cytotoxin genes were detected by PCR using novel species-specific primers. Among the 85 S. epidermidis and 84 S. haemolyticus isolates, 95.3% and 79.8%, respectively, carried at least one enterotoxin gene. The most frequent enterotoxin genes were sea (53.3%), seg (64.5%) and sei (67.5%). The seg gene was positively associated with S. epidermidis (p = 0.02), and this species was more toxigenic than S. haemolyticus. The hla/yidD gene was detected in 92.9% of S. epidermidis and the hla gene in 91.7% of S. haemolyticus isolates; hlb was detected in 92.9% of the S. epidermidis isolates and hld in 95.3%. Nosocomial Staphylococcus epidermidis and S. haemolyticus isolates exhibited a high toxigenic potential, mainly producing the non-classical enterotoxins seg and sei. The previously unreported detection of hla/yidD and hlb in S. epidermidis and S. haemolyticus using species-specific primers showed that these hemolysin genes differ between CoNS species and that they are highly frequent in blood culture isolates.

No MeSH data available.


Related in: MedlinePlus