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Taxonomic Characterization and Secondary Metabolite Profiling of Aspergillus Section Aspergillus Contaminating Feeds and Feedstuffs.

Greco M, Kemppainen M, Pose G, Pardo A - Toxins (Basel) (2015)

Bottom Line: All the isolates produced neoechinulin A, 17 isolates were positive for cladosporin and echinulin, and 18 were positive for neoechinulin B.Physcion and preechinulin were detected in a minor proportion of the isolates.This is the first report describing the detailed species composition and the secondary metabolite profiles of Aspergillus section Aspergillus contaminating animal feeds.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal 1876, Argentina. mariana.greco@gmail.com.

ABSTRACT
Xerophilic fungal species of the genus Aspergillus are economically highly relevant due to their ability to grow on low water activity substrates causing spoilage of stored goods and animal feeds. These fungi can synthesize a variety of secondary metabolites, many of which show animal toxicity, creating a health risk for food production animals and to humans as final consumers, respectively. Animal feeds used for rabbit, chinchilla and rainbow trout production in Argentina were analysed for the presence of xerophilic Aspergillus section Aspergillus species. High isolation frequencies (>60%) were detected in all the studied rabbit and chinchilla feeds, while the rainbow trout feeds showed lower fungal charge (25%). These section Aspergillus contaminations comprised predominantly five taxa. Twenty isolates were subjected to taxonomic characterization using both ascospore SEM micromorphology and two independent DNA loci sequencing. The secondary metabolite profiles of the isolates were determined qualitatively by HPLC-MS. All the isolates produced neoechinulin A, 17 isolates were positive for cladosporin and echinulin, and 18 were positive for neoechinulin B. Physcion and preechinulin were detected in a minor proportion of the isolates. This is the first report describing the detailed species composition and the secondary metabolite profiles of Aspergillus section Aspergillus contaminating animal feeds.

No MeSH data available.


Related in: MedlinePlus

Chromatograms and ESI spectrum of (a) Cladosporin (m/z 293); (b) Echinulin (m/z 462); (c) Neoechinulin A (m/z 324); (d) Neoechinulin B (m/z 322); (e) Preechinulin (m/z 326); (f) Physcion (m/z 285).
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toxins-07-03512-f003: Chromatograms and ESI spectrum of (a) Cladosporin (m/z 293); (b) Echinulin (m/z 462); (c) Neoechinulin A (m/z 324); (d) Neoechinulin B (m/z 322); (e) Preechinulin (m/z 326); (f) Physcion (m/z 285).

Mentions: Once the species identity was comprehensively established for the 20 isolates, their secondary metabolite profiles were analysed. The HPLC-MS analysis showed the presence of cladosporin, echinulin, and neoechinulin A and B (Table 5). All the studied isolates, representatives of the five species of section Aspergillus, were able to produce neoechinulin A. Eighteen isolates produced neoechinulin B, and 17 isolates were positive for cladosporin and echinulin. In addition, preechinulin and physcion were also detected. The Figure 3 shows representative chromatograms and the ESI spectrum of the determined secondary metabolites. None of the species showed a fixed profile, but a moderate intraspecies variation in the secondary metabolite production was detected.


Taxonomic Characterization and Secondary Metabolite Profiling of Aspergillus Section Aspergillus Contaminating Feeds and Feedstuffs.

Greco M, Kemppainen M, Pose G, Pardo A - Toxins (Basel) (2015)

Chromatograms and ESI spectrum of (a) Cladosporin (m/z 293); (b) Echinulin (m/z 462); (c) Neoechinulin A (m/z 324); (d) Neoechinulin B (m/z 322); (e) Preechinulin (m/z 326); (f) Physcion (m/z 285).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591650&req=5

toxins-07-03512-f003: Chromatograms and ESI spectrum of (a) Cladosporin (m/z 293); (b) Echinulin (m/z 462); (c) Neoechinulin A (m/z 324); (d) Neoechinulin B (m/z 322); (e) Preechinulin (m/z 326); (f) Physcion (m/z 285).
Mentions: Once the species identity was comprehensively established for the 20 isolates, their secondary metabolite profiles were analysed. The HPLC-MS analysis showed the presence of cladosporin, echinulin, and neoechinulin A and B (Table 5). All the studied isolates, representatives of the five species of section Aspergillus, were able to produce neoechinulin A. Eighteen isolates produced neoechinulin B, and 17 isolates were positive for cladosporin and echinulin. In addition, preechinulin and physcion were also detected. The Figure 3 shows representative chromatograms and the ESI spectrum of the determined secondary metabolites. None of the species showed a fixed profile, but a moderate intraspecies variation in the secondary metabolite production was detected.

Bottom Line: All the isolates produced neoechinulin A, 17 isolates were positive for cladosporin and echinulin, and 18 were positive for neoechinulin B.Physcion and preechinulin were detected in a minor proportion of the isolates.This is the first report describing the detailed species composition and the secondary metabolite profiles of Aspergillus section Aspergillus contaminating animal feeds.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal 1876, Argentina. mariana.greco@gmail.com.

ABSTRACT
Xerophilic fungal species of the genus Aspergillus are economically highly relevant due to their ability to grow on low water activity substrates causing spoilage of stored goods and animal feeds. These fungi can synthesize a variety of secondary metabolites, many of which show animal toxicity, creating a health risk for food production animals and to humans as final consumers, respectively. Animal feeds used for rabbit, chinchilla and rainbow trout production in Argentina were analysed for the presence of xerophilic Aspergillus section Aspergillus species. High isolation frequencies (>60%) were detected in all the studied rabbit and chinchilla feeds, while the rainbow trout feeds showed lower fungal charge (25%). These section Aspergillus contaminations comprised predominantly five taxa. Twenty isolates were subjected to taxonomic characterization using both ascospore SEM micromorphology and two independent DNA loci sequencing. The secondary metabolite profiles of the isolates were determined qualitatively by HPLC-MS. All the isolates produced neoechinulin A, 17 isolates were positive for cladosporin and echinulin, and 18 were positive for neoechinulin B. Physcion and preechinulin were detected in a minor proportion of the isolates. This is the first report describing the detailed species composition and the secondary metabolite profiles of Aspergillus section Aspergillus contaminating animal feeds.

No MeSH data available.


Related in: MedlinePlus