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Gold nanoparticle-labeled biosensor for rapid and sensitive detection of bacterial pathogens.

Wang Y, Alocilja EC - J Biol Eng (2015)

Bottom Line: By measuring the amount of AuNPs through an electrochemical method, the presence and the amount of the target bacteria were determined.Results showed a sensitivity of 10(1) colony forming units per milliliter (cfu/ml) with a linear range of 10(1)-10(6) cfu/ml.Compared to conventional culture plating methods, the biosensor reduced the detection time from 2 to 4 days to less than 1 hour with a simple target extraction method.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824 USA ; Present address: Division of Food Processing Science and Technology, U. S. Food and Drug Administration, Bedford Park, IL 60501 USA.

ABSTRACT

Background: Escherichia coli O157:H7 is one of the major foodborne bacterial pathogens and also a biodefense agent. To ensure food safety and public health, it is very important to develop rapid methods for E. coli O157:H7 detection. In this study, we designed a nanoparticle-labeled biosensor for the rapid detection of E. coli O157:H7 in broth.

Results: Magnetic nanoparticles (MNPs) were conjugated with monoclonal antibodies (Abs) to separate target E. coli O157:H7 cells from broth samples. Gold nanoparticles (AuNPs) were conjugated with polyclonal Abs, and were then introduced to the MNP-target complex to form a sandwich MNP-target-AuNP. By measuring the amount of AuNPs through an electrochemical method, the presence and the amount of the target bacteria were determined. Results showed a sensitivity of 10(1) colony forming units per milliliter (cfu/ml) with a linear range of 10(1)-10(6) cfu/ml.

Conclusions: Compared to conventional culture plating methods, the biosensor reduced the detection time from 2 to 4 days to less than 1 hour with a simple target extraction method. The AuNP-labeled biosensor has potential applications in the rapid detection of infectious agents for public health, biodefense, and food/water safety.

No MeSH data available.


Related in: MedlinePlus

Differential pulse voltammetric sensorgrams of the gold nanoparticle (AuNPs) and Polyaniline (PANI)-coated magnetic nanoparticles (MNPs). AuNPs show a current peak at 0.3 V and MNPs show a current peak at 0.58 V
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Fig4: Differential pulse voltammetric sensorgrams of the gold nanoparticle (AuNPs) and Polyaniline (PANI)-coated magnetic nanoparticles (MNPs). AuNPs show a current peak at 0.3 V and MNPs show a current peak at 0.58 V

Mentions: Figure 4 shows two typical sensorgrams of native AuNPs and native MNPs. Current peak for AuNPs is at 0.3 V and MNPs is at 0.58 V. Figure 5 shows typical DPV sensorgrams for the detection of E. coli O157:H7 in different cell concentrations (102, 104, and 106 colony forming units per milliliter, cfu/ml). The sensorgrams show a wide curve that seems to include both AuNPs and MNPs. For the analysis, peak current to the left (representing AuNPs) around 0.3 V was chosen for signal reporting. As shown in the graph, peak current for AuNPs increased with increasing cell concentration. Figure 5 confirms the formation of the MNP-cell-AuNP complex. The amount of target cells detected was proportional to the amount of AuNPs.Fig. 4


Gold nanoparticle-labeled biosensor for rapid and sensitive detection of bacterial pathogens.

Wang Y, Alocilja EC - J Biol Eng (2015)

Differential pulse voltammetric sensorgrams of the gold nanoparticle (AuNPs) and Polyaniline (PANI)-coated magnetic nanoparticles (MNPs). AuNPs show a current peak at 0.3 V and MNPs show a current peak at 0.58 V
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4591638&req=5

Fig4: Differential pulse voltammetric sensorgrams of the gold nanoparticle (AuNPs) and Polyaniline (PANI)-coated magnetic nanoparticles (MNPs). AuNPs show a current peak at 0.3 V and MNPs show a current peak at 0.58 V
Mentions: Figure 4 shows two typical sensorgrams of native AuNPs and native MNPs. Current peak for AuNPs is at 0.3 V and MNPs is at 0.58 V. Figure 5 shows typical DPV sensorgrams for the detection of E. coli O157:H7 in different cell concentrations (102, 104, and 106 colony forming units per milliliter, cfu/ml). The sensorgrams show a wide curve that seems to include both AuNPs and MNPs. For the analysis, peak current to the left (representing AuNPs) around 0.3 V was chosen for signal reporting. As shown in the graph, peak current for AuNPs increased with increasing cell concentration. Figure 5 confirms the formation of the MNP-cell-AuNP complex. The amount of target cells detected was proportional to the amount of AuNPs.Fig. 4

Bottom Line: By measuring the amount of AuNPs through an electrochemical method, the presence and the amount of the target bacteria were determined.Results showed a sensitivity of 10(1) colony forming units per milliliter (cfu/ml) with a linear range of 10(1)-10(6) cfu/ml.Compared to conventional culture plating methods, the biosensor reduced the detection time from 2 to 4 days to less than 1 hour with a simple target extraction method.

View Article: PubMed Central - PubMed

Affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824 USA ; Present address: Division of Food Processing Science and Technology, U. S. Food and Drug Administration, Bedford Park, IL 60501 USA.

ABSTRACT

Background: Escherichia coli O157:H7 is one of the major foodborne bacterial pathogens and also a biodefense agent. To ensure food safety and public health, it is very important to develop rapid methods for E. coli O157:H7 detection. In this study, we designed a nanoparticle-labeled biosensor for the rapid detection of E. coli O157:H7 in broth.

Results: Magnetic nanoparticles (MNPs) were conjugated with monoclonal antibodies (Abs) to separate target E. coli O157:H7 cells from broth samples. Gold nanoparticles (AuNPs) were conjugated with polyclonal Abs, and were then introduced to the MNP-target complex to form a sandwich MNP-target-AuNP. By measuring the amount of AuNPs through an electrochemical method, the presence and the amount of the target bacteria were determined. Results showed a sensitivity of 10(1) colony forming units per milliliter (cfu/ml) with a linear range of 10(1)-10(6) cfu/ml.

Conclusions: Compared to conventional culture plating methods, the biosensor reduced the detection time from 2 to 4 days to less than 1 hour with a simple target extraction method. The AuNP-labeled biosensor has potential applications in the rapid detection of infectious agents for public health, biodefense, and food/water safety.

No MeSH data available.


Related in: MedlinePlus