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Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus.

Xu Q, Zhao G, Lin J, Wang Q, Hu L, Jiang Z - BMC Ophthalmol (2015)

Bottom Line: After 8 h of fungal stimulations, IL-6 and CXCL1 of pretreatment groups were still significantly (p < 0.05) lower than fungal groups.With progress of fungus stimulation, expression of IL-1β,CXCL1 ,CXCL2,MCP-1 gradually increased, whilepretreated with Laminarin to block Dectin-1, these expression decreased, indicating that Dectin-1 maypromote immune reaction through them.IL-10 decreased in fungal group because of itsimmunosuppressive effect at 4h, and it began to increase at 8h to suppress Th1 inflammation response inorder to avoid excessive tissue damage.

View Article: PubMed Central - PubMed

Affiliation: Affiliated Hospital of Qingdao University, 16th Jiangsu Road, Qingdao, Shandong Province, China. yankexuqiang@126.com.

ABSTRACT

Background: To observe Dectin-1 expression in fungal keratitis on rat models and to determine the role of Dectin-1 in innate immune response to Aspergillus fumigatus.

Methods: Wistar rats were randomly divided into control, fungal keratitis and pretreatment (pretreated with Laminarin) groups. Samples were used for conducting immunohistochemical staining and real-time PCR to observe expression of cytokines like CCL2, CCL3, CXCL1, CXCL2, IL-1β, TNF-α, IL-6, IL-10.

Results: After fungal stimulations, all 7 inflammatory factors, except IL-10, increased with different levels. After 4 h of fungal stimulations, IL-1β, IL-6, CCL2, CXCL1 and CXCL2 of pretreatment groups were significantly (p < 0.05) lower than fungal groups, while the other 3 cytokines had no significant changes. After 8 h of fungal stimulations, IL-6 and CXCL1 of pretreatment groups were still significantly (p < 0.05) lower than fungal groups.

Discussion: With progress of fungus stimulation, expression of IL-1β,CXCL1 ,CXCL2,MCP-1 gradually increased, whilepretreated with Laminarin to block Dectin-1, these expression decreased, indicating that Dectin-1 maypromote immune reaction through them. IL-10 decreased in fungal group because of itsimmunosuppressive effect at 4h, and it began to increase at 8h to suppress Th1 inflammation response inorder to avoid excessive tissue damage.

Conclusion: Dectin-1 in early period of innate immune responses in rat fungal keratitis might work through IL-1β, IL-6, CCL2, CXCL1, CXCL2 to recruit neutrophils and macrophages to participate anti-fungal immunity.

No MeSH data available.


Related in: MedlinePlus

Immunocytochemistry results immunohistochemical staining × 400. a normal rat group b fungal group. The eight inflammatory factors were mainly expressed in cytoplasm in the corneal epithelium and shallow stroma. The fungal groups and pretreatment groups showed clear pictures by comparing with normal rat groups, while there were no obvious difference between fungal groups and pretreatment groups
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Fig2: Immunocytochemistry results immunohistochemical staining × 400. a normal rat group b fungal group. The eight inflammatory factors were mainly expressed in cytoplasm in the corneal epithelium and shallow stroma. The fungal groups and pretreatment groups showed clear pictures by comparing with normal rat groups, while there were no obvious difference between fungal groups and pretreatment groups

Mentions: The changes of corneas on rat models after fungal stimulation in different time points were observed as showed in Fig. 1. Positive results of immunohistochemical staining can be seen in the cell. The greater inflammatory factors had deeper color in tissues. Results demonstrated that eight inflammatory factors were mainly expressed in cytoplasm in the corneal epithelium and shallow stroma. The fungal groups and pretreatment groups showed clear pictures by comparing with normal rat groups, while there were no obvious difference between fungal groups and pretreatment groups (Fig. 2).Fig. 1


Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus.

Xu Q, Zhao G, Lin J, Wang Q, Hu L, Jiang Z - BMC Ophthalmol (2015)

Immunocytochemistry results immunohistochemical staining × 400. a normal rat group b fungal group. The eight inflammatory factors were mainly expressed in cytoplasm in the corneal epithelium and shallow stroma. The fungal groups and pretreatment groups showed clear pictures by comparing with normal rat groups, while there were no obvious difference between fungal groups and pretreatment groups
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4591637&req=5

Fig2: Immunocytochemistry results immunohistochemical staining × 400. a normal rat group b fungal group. The eight inflammatory factors were mainly expressed in cytoplasm in the corneal epithelium and shallow stroma. The fungal groups and pretreatment groups showed clear pictures by comparing with normal rat groups, while there were no obvious difference between fungal groups and pretreatment groups
Mentions: The changes of corneas on rat models after fungal stimulation in different time points were observed as showed in Fig. 1. Positive results of immunohistochemical staining can be seen in the cell. The greater inflammatory factors had deeper color in tissues. Results demonstrated that eight inflammatory factors were mainly expressed in cytoplasm in the corneal epithelium and shallow stroma. The fungal groups and pretreatment groups showed clear pictures by comparing with normal rat groups, while there were no obvious difference between fungal groups and pretreatment groups (Fig. 2).Fig. 1

Bottom Line: After 8 h of fungal stimulations, IL-6 and CXCL1 of pretreatment groups were still significantly (p < 0.05) lower than fungal groups.With progress of fungus stimulation, expression of IL-1β,CXCL1 ,CXCL2,MCP-1 gradually increased, whilepretreated with Laminarin to block Dectin-1, these expression decreased, indicating that Dectin-1 maypromote immune reaction through them.IL-10 decreased in fungal group because of itsimmunosuppressive effect at 4h, and it began to increase at 8h to suppress Th1 inflammation response inorder to avoid excessive tissue damage.

View Article: PubMed Central - PubMed

Affiliation: Affiliated Hospital of Qingdao University, 16th Jiangsu Road, Qingdao, Shandong Province, China. yankexuqiang@126.com.

ABSTRACT

Background: To observe Dectin-1 expression in fungal keratitis on rat models and to determine the role of Dectin-1 in innate immune response to Aspergillus fumigatus.

Methods: Wistar rats were randomly divided into control, fungal keratitis and pretreatment (pretreated with Laminarin) groups. Samples were used for conducting immunohistochemical staining and real-time PCR to observe expression of cytokines like CCL2, CCL3, CXCL1, CXCL2, IL-1β, TNF-α, IL-6, IL-10.

Results: After fungal stimulations, all 7 inflammatory factors, except IL-10, increased with different levels. After 4 h of fungal stimulations, IL-1β, IL-6, CCL2, CXCL1 and CXCL2 of pretreatment groups were significantly (p < 0.05) lower than fungal groups, while the other 3 cytokines had no significant changes. After 8 h of fungal stimulations, IL-6 and CXCL1 of pretreatment groups were still significantly (p < 0.05) lower than fungal groups.

Discussion: With progress of fungus stimulation, expression of IL-1β,CXCL1 ,CXCL2,MCP-1 gradually increased, whilepretreated with Laminarin to block Dectin-1, these expression decreased, indicating that Dectin-1 maypromote immune reaction through them. IL-10 decreased in fungal group because of itsimmunosuppressive effect at 4h, and it began to increase at 8h to suppress Th1 inflammation response inorder to avoid excessive tissue damage.

Conclusion: Dectin-1 in early period of innate immune responses in rat fungal keratitis might work through IL-1β, IL-6, CCL2, CXCL1, CXCL2 to recruit neutrophils and macrophages to participate anti-fungal immunity.

No MeSH data available.


Related in: MedlinePlus