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Specific detection of OCT4 isoforms in inflammatory bowel disease.

Maragkoudaki M, Vaiopoulou A, Theodoropoulos GE, Legaki E, Sechi LA, Karamanolis G, Zografos G, Gazouli M - Gut Pathog (2015)

Bottom Line: In CD patients only SOX-2 mRNA levels were found slightly increased compared to healthy controls.Our results suggest that OCT4 is expressed in patients with IBD.Furthermore, we found the presence of the OCT4B1 isoform in IBD in both tissue and blood samples.

View Article: PubMed Central - PubMed

Affiliation: First Department of Pediatrics, Athens University Medical School, "Aghia Sophia" Children's Hospital, Athens, Greece.

ABSTRACT

Background: Developmentally early cells are mobilized into peripheral blood in Crohn's disease (CD) patients. OCT4, is considered to be important in sustaining the pluripotency of stem cells. OCT4 splicing variants are differentially expressed in pluripotent and non-pluripotent cells. Our study aims to investigate the expression pattern of OCT4 variants and SOX-2, an essential factor implicated in self-renewal and pluripotency, in tissue and blood samples from patients with IBD.

Methods: Peripheral blood and tissue samples were collected from patients with active CD and ulcerative colitis (UC), and from healthy individuals. OCT4 expression was documented by Western blot, immunohistochemistry and by reverse transcription-real-time PCR. OCT4 isoform determination was documented using specific primers. SOX-2 expression levels were also evaluated.

Results: OCT4 protein levels were significantly higher in CD tissue samples than in CD blood samples, and in UC tissue samples. OCT4 protein was localized mainly in the cytosol. In all samples, only the OCT4 pseudogenes and the OCT4B1 variant were detected. OCT4B1 expression levels were elevated in both tissue and blood samples from CD and UC cases compared to healthy controls. In CD patients only SOX-2 mRNA levels were found slightly increased compared to healthy controls.

Conclusion: Our results suggest that OCT4 is expressed in patients with IBD. Furthermore, we found the presence of the OCT4B1 isoform in IBD in both tissue and blood samples. Our results have shown, that developmentally early cells might be mobilized into peripheral blood as result of tissue damage, indicating a possible role of these cells in repair of injured intestinal tract.

No MeSH data available.


Related in: MedlinePlus

Representative figure of Western blot analysis of OCT4 (a), SOX2 (b) and b-actin (c) proteins, and relative expression of OCT4 and SOX2. Samples 1 CD tissue, 2 CD blood, 3 UC tissue, 4 UC blood. *p < 0.05; **p < 0.01
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Fig1: Representative figure of Western blot analysis of OCT4 (a), SOX2 (b) and b-actin (c) proteins, and relative expression of OCT4 and SOX2. Samples 1 CD tissue, 2 CD blood, 3 UC tissue, 4 UC blood. *p < 0.05; **p < 0.01

Mentions: We first analysed the expression of OCT4 protein levels in blood and tissue samples from CD and UC patients. As indicated in Fig. 1, OCT4 was expressed in both tissue and blood samples from CD and UC cases. OCT4 protein levels were significantly higher in CD tissue samples compared to CD blood samples (p < 0.05) and to UC tissue samples (p < 0.01). We also examined the expression of the ESC marker SOX-2 with the aim of determine if the OCT4 expression pattern related to a stem cell phenotype also. Similarly SOX-2 was significantly higher in CD tissue samples compared to CD blood samples (p < 0.05) and to UC tissue samples (p < 0.05). Interestingly, as indicated in Fig. 2 OCT4 protein was expressed mainly in the cytosol in both CD and UC cases, which is different from the description about OCT4 as a nuclear protein. It is known that the different OCT4 isoforms can be distinguished by their different subcellular localization.Fig. 1


Specific detection of OCT4 isoforms in inflammatory bowel disease.

Maragkoudaki M, Vaiopoulou A, Theodoropoulos GE, Legaki E, Sechi LA, Karamanolis G, Zografos G, Gazouli M - Gut Pathog (2015)

Representative figure of Western blot analysis of OCT4 (a), SOX2 (b) and b-actin (c) proteins, and relative expression of OCT4 and SOX2. Samples 1 CD tissue, 2 CD blood, 3 UC tissue, 4 UC blood. *p < 0.05; **p < 0.01
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4591585&req=5

Fig1: Representative figure of Western blot analysis of OCT4 (a), SOX2 (b) and b-actin (c) proteins, and relative expression of OCT4 and SOX2. Samples 1 CD tissue, 2 CD blood, 3 UC tissue, 4 UC blood. *p < 0.05; **p < 0.01
Mentions: We first analysed the expression of OCT4 protein levels in blood and tissue samples from CD and UC patients. As indicated in Fig. 1, OCT4 was expressed in both tissue and blood samples from CD and UC cases. OCT4 protein levels were significantly higher in CD tissue samples compared to CD blood samples (p < 0.05) and to UC tissue samples (p < 0.01). We also examined the expression of the ESC marker SOX-2 with the aim of determine if the OCT4 expression pattern related to a stem cell phenotype also. Similarly SOX-2 was significantly higher in CD tissue samples compared to CD blood samples (p < 0.05) and to UC tissue samples (p < 0.05). Interestingly, as indicated in Fig. 2 OCT4 protein was expressed mainly in the cytosol in both CD and UC cases, which is different from the description about OCT4 as a nuclear protein. It is known that the different OCT4 isoforms can be distinguished by their different subcellular localization.Fig. 1

Bottom Line: In CD patients only SOX-2 mRNA levels were found slightly increased compared to healthy controls.Our results suggest that OCT4 is expressed in patients with IBD.Furthermore, we found the presence of the OCT4B1 isoform in IBD in both tissue and blood samples.

View Article: PubMed Central - PubMed

Affiliation: First Department of Pediatrics, Athens University Medical School, "Aghia Sophia" Children's Hospital, Athens, Greece.

ABSTRACT

Background: Developmentally early cells are mobilized into peripheral blood in Crohn's disease (CD) patients. OCT4, is considered to be important in sustaining the pluripotency of stem cells. OCT4 splicing variants are differentially expressed in pluripotent and non-pluripotent cells. Our study aims to investigate the expression pattern of OCT4 variants and SOX-2, an essential factor implicated in self-renewal and pluripotency, in tissue and blood samples from patients with IBD.

Methods: Peripheral blood and tissue samples were collected from patients with active CD and ulcerative colitis (UC), and from healthy individuals. OCT4 expression was documented by Western blot, immunohistochemistry and by reverse transcription-real-time PCR. OCT4 isoform determination was documented using specific primers. SOX-2 expression levels were also evaluated.

Results: OCT4 protein levels were significantly higher in CD tissue samples than in CD blood samples, and in UC tissue samples. OCT4 protein was localized mainly in the cytosol. In all samples, only the OCT4 pseudogenes and the OCT4B1 variant were detected. OCT4B1 expression levels were elevated in both tissue and blood samples from CD and UC cases compared to healthy controls. In CD patients only SOX-2 mRNA levels were found slightly increased compared to healthy controls.

Conclusion: Our results suggest that OCT4 is expressed in patients with IBD. Furthermore, we found the presence of the OCT4B1 isoform in IBD in both tissue and blood samples. Our results have shown, that developmentally early cells might be mobilized into peripheral blood as result of tissue damage, indicating a possible role of these cells in repair of injured intestinal tract.

No MeSH data available.


Related in: MedlinePlus