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Probiotic and anti-inflammatory attributes of an isolate Lactobacillus helveticus NS8 from Mongolian fermented koumiss.

Rong J, Zheng H, Liu M, Hu X, Wang T, Zhang X, Jin F, Wang L - BMC Microbiol. (2015)

Bottom Line: NS8 was found to be rather protective against TNBS (2,4,6-trinitrobenzene sulfonic acid)-induced murine colitis.Furthermore, NS8 was also able to diminish the proinflammatory effects of lipopolysaccharide (LPS) in mouse macrophage cell line RAW264.7 by inducing higher levels of IL-10.In summary, L. helveticus NS8 exhibited good probiotic and particularly immunomodulatory properties, with a potential for development of functional food commercially or therapeutic adjuvant for inflammatory diseases.

View Article: PubMed Central - PubMed

Affiliation: The Affiliated Hospital, School of Medicine, Hangzhou Normal University, Hangzhou, China. jjrong@hznu.edu.cn.

ABSTRACT

Background: Koumiss is a traditionally fermented mare's milk described with health-promoting potentials for decades. However, only a few studies focused on the probiotic strains isolated from koumiss. In this study, we collected koumiss samples from Inner Mongolian pasturing area of China and selected a promising strain of Lactobacillus helveticus, isolate NS8, based on the survival abilities in gastrointestinal tract (GIT) and adhesion to intestinal endothelial cells in vitro. As the ability to positively modulate host immune response is a feature of increasing importance in measuring the probiotic potential of a bacterial strain, our study mainly focus on the immunomodulatory properties of L. helveticus NS8 by using in vivo and ex vivo analyses.

Results: L. helveticus NS8 was identified by molecular-typing methods, both at genus and species levels. As a typical food niche-specific bacteria, NS8 showed a moderate survival ability in GIT environment in vitro. However, an excellent binding capacity to the human intestinal epithelial cells, along with significant autoaggregation and cell-surface hydrophobicity was observed. Additionally, the presence of S-layer protein was responsible for the cell surface properties of this strain. NS8 was found to be rather protective against TNBS (2,4,6-trinitrobenzene sulfonic acid)-induced murine colitis. In the meantime, co-culture with NS8 induced an increased level of secretion of anti-inflammatory cytokine IL-10 in peripheral blood mono-nuclear cells (PBMCs). Furthermore, NS8 was also able to diminish the proinflammatory effects of lipopolysaccharide (LPS) in mouse macrophage cell line RAW264.7 by inducing higher levels of IL-10. Specially, adding of the purified S-layer protein didn't influence the production of IL-10. The specific ligand-host receptor interactions on the NS8 specific immune responses need to be learned further.

Conclusion: In summary, L. helveticus NS8 exhibited good probiotic and particularly immunomodulatory properties, with a potential for development of functional food commercially or therapeutic adjuvant for inflammatory diseases.

No MeSH data available.


Related in: MedlinePlus

Cytokine response of human PBMCs to stimulation with L. helveticus NS8 and L. casei Shirota strains. Cell supernatant were collected after 24 h co-incubation with bacteria (bacteria: cell ratio of 100:1), and the release of IL-10 (a) and IL-12p70 (b) and TNF-α (c) were assayed by ELISA. Results were represented as means ± SEM pg/mL for 9 independent healthy donors
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Fig4: Cytokine response of human PBMCs to stimulation with L. helveticus NS8 and L. casei Shirota strains. Cell supernatant were collected after 24 h co-incubation with bacteria (bacteria: cell ratio of 100:1), and the release of IL-10 (a) and IL-12p70 (b) and TNF-α (c) were assayed by ELISA. Results were represented as means ± SEM pg/mL for 9 independent healthy donors

Mentions: We investigated the cytokine profiles stimulated by NS8 upon PBMCs collected from 9 independent donors. Although the absolute cytokine concentrations released by PBMCs varied between different donors, NS8 induced more significant increase of anti-inflammatory cytokine IL10, while less impact on release of proinflammatory cytokine IL12, in comparation with probiotic strain L. casei Shirota, which has been well characterized with health-promoting functions (Fig. 4). The ratio of IL10 to IL12 production was higher for NS8 (23.5) than L. casei Shirota (3.4). Both strains were able to induce TNF-α secretion from stimulated PBMCs, but the induction of this cytokine was partly lower when PBMCs were stimulated by NS8. These results indicated that IL-10 producing immune cells could evidently sense the stimulation of NS8.Fig. 4


Probiotic and anti-inflammatory attributes of an isolate Lactobacillus helveticus NS8 from Mongolian fermented koumiss.

Rong J, Zheng H, Liu M, Hu X, Wang T, Zhang X, Jin F, Wang L - BMC Microbiol. (2015)

Cytokine response of human PBMCs to stimulation with L. helveticus NS8 and L. casei Shirota strains. Cell supernatant were collected after 24 h co-incubation with bacteria (bacteria: cell ratio of 100:1), and the release of IL-10 (a) and IL-12p70 (b) and TNF-α (c) were assayed by ELISA. Results were represented as means ± SEM pg/mL for 9 independent healthy donors
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4591576&req=5

Fig4: Cytokine response of human PBMCs to stimulation with L. helveticus NS8 and L. casei Shirota strains. Cell supernatant were collected after 24 h co-incubation with bacteria (bacteria: cell ratio of 100:1), and the release of IL-10 (a) and IL-12p70 (b) and TNF-α (c) were assayed by ELISA. Results were represented as means ± SEM pg/mL for 9 independent healthy donors
Mentions: We investigated the cytokine profiles stimulated by NS8 upon PBMCs collected from 9 independent donors. Although the absolute cytokine concentrations released by PBMCs varied between different donors, NS8 induced more significant increase of anti-inflammatory cytokine IL10, while less impact on release of proinflammatory cytokine IL12, in comparation with probiotic strain L. casei Shirota, which has been well characterized with health-promoting functions (Fig. 4). The ratio of IL10 to IL12 production was higher for NS8 (23.5) than L. casei Shirota (3.4). Both strains were able to induce TNF-α secretion from stimulated PBMCs, but the induction of this cytokine was partly lower when PBMCs were stimulated by NS8. These results indicated that IL-10 producing immune cells could evidently sense the stimulation of NS8.Fig. 4

Bottom Line: NS8 was found to be rather protective against TNBS (2,4,6-trinitrobenzene sulfonic acid)-induced murine colitis.Furthermore, NS8 was also able to diminish the proinflammatory effects of lipopolysaccharide (LPS) in mouse macrophage cell line RAW264.7 by inducing higher levels of IL-10.In summary, L. helveticus NS8 exhibited good probiotic and particularly immunomodulatory properties, with a potential for development of functional food commercially or therapeutic adjuvant for inflammatory diseases.

View Article: PubMed Central - PubMed

Affiliation: The Affiliated Hospital, School of Medicine, Hangzhou Normal University, Hangzhou, China. jjrong@hznu.edu.cn.

ABSTRACT

Background: Koumiss is a traditionally fermented mare's milk described with health-promoting potentials for decades. However, only a few studies focused on the probiotic strains isolated from koumiss. In this study, we collected koumiss samples from Inner Mongolian pasturing area of China and selected a promising strain of Lactobacillus helveticus, isolate NS8, based on the survival abilities in gastrointestinal tract (GIT) and adhesion to intestinal endothelial cells in vitro. As the ability to positively modulate host immune response is a feature of increasing importance in measuring the probiotic potential of a bacterial strain, our study mainly focus on the immunomodulatory properties of L. helveticus NS8 by using in vivo and ex vivo analyses.

Results: L. helveticus NS8 was identified by molecular-typing methods, both at genus and species levels. As a typical food niche-specific bacteria, NS8 showed a moderate survival ability in GIT environment in vitro. However, an excellent binding capacity to the human intestinal epithelial cells, along with significant autoaggregation and cell-surface hydrophobicity was observed. Additionally, the presence of S-layer protein was responsible for the cell surface properties of this strain. NS8 was found to be rather protective against TNBS (2,4,6-trinitrobenzene sulfonic acid)-induced murine colitis. In the meantime, co-culture with NS8 induced an increased level of secretion of anti-inflammatory cytokine IL-10 in peripheral blood mono-nuclear cells (PBMCs). Furthermore, NS8 was also able to diminish the proinflammatory effects of lipopolysaccharide (LPS) in mouse macrophage cell line RAW264.7 by inducing higher levels of IL-10. Specially, adding of the purified S-layer protein didn't influence the production of IL-10. The specific ligand-host receptor interactions on the NS8 specific immune responses need to be learned further.

Conclusion: In summary, L. helveticus NS8 exhibited good probiotic and particularly immunomodulatory properties, with a potential for development of functional food commercially or therapeutic adjuvant for inflammatory diseases.

No MeSH data available.


Related in: MedlinePlus