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Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean.

Devi MJ, Sinclair TR, Taliercio E - PLoS ONE (2015)

Bottom Line: The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes.TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa.The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes. Hutcheson, a fast-wilting soybean, and PI 471938, a slow-wilting soybean, respond to increased VPD with a linear increase in TR. TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa. The objective of this study was to identify the response of the transcriptome of these accessions to elevated VPD under well-watered conditions and identify responses that are unique to the slow-wilting accessions. Gene expression analysis in leaves of genotypes PI 471938 and Hutcheson showed that 22 and 1 genes, respectively, were differentially expressed under high VPD. In contrast, there were 944 genes differentially expressed in PI 416937 with the same increase in VPD. The increased alteration of the transcriptome of PI 416937 in response to elevated VPD clearly distinguished it from the other slow-wilting PI 471938 and the fast-wilting Hutcheson. The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

No MeSH data available.


Metabolic overview of genes differentially regulated in PI 416937 due to high VPD.Color bar ranging from green to red represents log2 fold from down-regulated (-10.5) to up-regulated (+10.5) using MapMan.
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pone.0139134.g006: Metabolic overview of genes differentially regulated in PI 416937 due to high VPD.Color bar ranging from green to red represents log2 fold from down-regulated (-10.5) to up-regulated (+10.5) using MapMan.

Mentions: Eighty-eight genes were assigned to the cell wall BIN, of which, 74 were down-regulated and 14 were up-regulated. Most of these genes are related to cellulose synthesis, cell wall precursors, cell wall modification, pectin esterases, pectate lyases and polygalacturonases (Figs 5 and 6). It is evident from both GO analysis and MapMan that expression of many genes related to the cell wall was sensitive to alterations in VPD. Reduction in the expression of expansin and extensin genes is consistent with the observed reduction in leaf expansion under prolonged high VPD conditions observed in PI 416937 [33]. Decrease in leaf area is a common response to drought in many crops. For example, leaf area decrease was found in drought-tolerant rice cultivar Moroberekan, which showed down-regulation of cell wall depository genes under drought conditions compared to a sensitive variety [34].


Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean.

Devi MJ, Sinclair TR, Taliercio E - PLoS ONE (2015)

Metabolic overview of genes differentially regulated in PI 416937 due to high VPD.Color bar ranging from green to red represents log2 fold from down-regulated (-10.5) to up-regulated (+10.5) using MapMan.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591296&req=5

pone.0139134.g006: Metabolic overview of genes differentially regulated in PI 416937 due to high VPD.Color bar ranging from green to red represents log2 fold from down-regulated (-10.5) to up-regulated (+10.5) using MapMan.
Mentions: Eighty-eight genes were assigned to the cell wall BIN, of which, 74 were down-regulated and 14 were up-regulated. Most of these genes are related to cellulose synthesis, cell wall precursors, cell wall modification, pectin esterases, pectate lyases and polygalacturonases (Figs 5 and 6). It is evident from both GO analysis and MapMan that expression of many genes related to the cell wall was sensitive to alterations in VPD. Reduction in the expression of expansin and extensin genes is consistent with the observed reduction in leaf expansion under prolonged high VPD conditions observed in PI 416937 [33]. Decrease in leaf area is a common response to drought in many crops. For example, leaf area decrease was found in drought-tolerant rice cultivar Moroberekan, which showed down-regulation of cell wall depository genes under drought conditions compared to a sensitive variety [34].

Bottom Line: The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes.TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa.The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes. Hutcheson, a fast-wilting soybean, and PI 471938, a slow-wilting soybean, respond to increased VPD with a linear increase in TR. TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa. The objective of this study was to identify the response of the transcriptome of these accessions to elevated VPD under well-watered conditions and identify responses that are unique to the slow-wilting accessions. Gene expression analysis in leaves of genotypes PI 471938 and Hutcheson showed that 22 and 1 genes, respectively, were differentially expressed under high VPD. In contrast, there were 944 genes differentially expressed in PI 416937 with the same increase in VPD. The increased alteration of the transcriptome of PI 416937 in response to elevated VPD clearly distinguished it from the other slow-wilting PI 471938 and the fast-wilting Hutcheson. The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

No MeSH data available.