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Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean.

Devi MJ, Sinclair TR, Taliercio E - PLoS ONE (2015)

Bottom Line: The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes.TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa.The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes. Hutcheson, a fast-wilting soybean, and PI 471938, a slow-wilting soybean, respond to increased VPD with a linear increase in TR. TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa. The objective of this study was to identify the response of the transcriptome of these accessions to elevated VPD under well-watered conditions and identify responses that are unique to the slow-wilting accessions. Gene expression analysis in leaves of genotypes PI 471938 and Hutcheson showed that 22 and 1 genes, respectively, were differentially expressed under high VPD. In contrast, there were 944 genes differentially expressed in PI 416937 with the same increase in VPD. The increased alteration of the transcriptome of PI 416937 in response to elevated VPD clearly distinguished it from the other slow-wilting PI 471938 and the fast-wilting Hutcheson. The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

No MeSH data available.


Overview of the stress related genes differentially regulated in PI 416937 exposed to high VPD.Gene transcripts that are induced or repressed due to high VPD are shown in red and green colors, respectively, as shown in the color bar ranging from -10.5 to +10.5.
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pone.0139134.g005: Overview of the stress related genes differentially regulated in PI 416937 exposed to high VPD.Gene transcripts that are induced or repressed due to high VPD are shown in red and green colors, respectively, as shown in the color bar ranging from -10.5 to +10.5.

Mentions: Several Transcription Factor (TF) genes were differentially regulated in PI 416937 under high VPD conditions relative to low VPD. These TFs play essential roles in regulating stress responsive genes in various signal transduction pathways [14]. Seventy-nine genes annotated as transcription factors were differentially regulated in PI 416937 in response to high VPD; 40 were up-regulated, and 39 were down-regulated. TF genes coding for the zinc-finger family proteins, MYB domain containing family, WRKY, AP2-EREBP/ethylene response factor (ERF), bZIP (Fig 5) and NAC were identified as differentially regulated. The expression of selected transcription factors were validated, including a WRKY, bZIP, NAC and MYB TF (Fig 3A).


Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean.

Devi MJ, Sinclair TR, Taliercio E - PLoS ONE (2015)

Overview of the stress related genes differentially regulated in PI 416937 exposed to high VPD.Gene transcripts that are induced or repressed due to high VPD are shown in red and green colors, respectively, as shown in the color bar ranging from -10.5 to +10.5.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591296&req=5

pone.0139134.g005: Overview of the stress related genes differentially regulated in PI 416937 exposed to high VPD.Gene transcripts that are induced or repressed due to high VPD are shown in red and green colors, respectively, as shown in the color bar ranging from -10.5 to +10.5.
Mentions: Several Transcription Factor (TF) genes were differentially regulated in PI 416937 under high VPD conditions relative to low VPD. These TFs play essential roles in regulating stress responsive genes in various signal transduction pathways [14]. Seventy-nine genes annotated as transcription factors were differentially regulated in PI 416937 in response to high VPD; 40 were up-regulated, and 39 were down-regulated. TF genes coding for the zinc-finger family proteins, MYB domain containing family, WRKY, AP2-EREBP/ethylene response factor (ERF), bZIP (Fig 5) and NAC were identified as differentially regulated. The expression of selected transcription factors were validated, including a WRKY, bZIP, NAC and MYB TF (Fig 3A).

Bottom Line: The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes.TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa.The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes. Hutcheson, a fast-wilting soybean, and PI 471938, a slow-wilting soybean, respond to increased VPD with a linear increase in TR. TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa. The objective of this study was to identify the response of the transcriptome of these accessions to elevated VPD under well-watered conditions and identify responses that are unique to the slow-wilting accessions. Gene expression analysis in leaves of genotypes PI 471938 and Hutcheson showed that 22 and 1 genes, respectively, were differentially expressed under high VPD. In contrast, there were 944 genes differentially expressed in PI 416937 with the same increase in VPD. The increased alteration of the transcriptome of PI 416937 in response to elevated VPD clearly distinguished it from the other slow-wilting PI 471938 and the fast-wilting Hutcheson. The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

No MeSH data available.