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Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean.

Devi MJ, Sinclair TR, Taliercio E - PLoS ONE (2015)

Bottom Line: The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes.TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa.The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes. Hutcheson, a fast-wilting soybean, and PI 471938, a slow-wilting soybean, respond to increased VPD with a linear increase in TR. TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa. The objective of this study was to identify the response of the transcriptome of these accessions to elevated VPD under well-watered conditions and identify responses that are unique to the slow-wilting accessions. Gene expression analysis in leaves of genotypes PI 471938 and Hutcheson showed that 22 and 1 genes, respectively, were differentially expressed under high VPD. In contrast, there were 944 genes differentially expressed in PI 416937 with the same increase in VPD. The increased alteration of the transcriptome of PI 416937 in response to elevated VPD clearly distinguished it from the other slow-wilting PI 471938 and the fast-wilting Hutcheson. The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

No MeSH data available.


GO terms significantly over-represented (higher percentage) in the differentially expressed genes list compared to background/reference genes (all expressed genes) list in PI 416937 under high VPD conditions.Green bars represent percentage of genes belongs to GO categories from total number of up- and down-regulated genes due to high VPD, and red bars indicate percentage of genes belong to GO category from total number of background/reference list in PI 416937.
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pone.0139134.g004: GO terms significantly over-represented (higher percentage) in the differentially expressed genes list compared to background/reference genes (all expressed genes) list in PI 416937 under high VPD conditions.Green bars represent percentage of genes belongs to GO categories from total number of up- and down-regulated genes due to high VPD, and red bars indicate percentage of genes belong to GO category from total number of background/reference list in PI 416937.

Mentions: In contrast to PI 471938 and Hutcheson, genotype PI 416937 differentially regulated 944 genes when VPD was elevated. Of the 944 differentially regulated genes, 425 were up-regulated and 519 genes were down-regulated (Fig 2 and S3 Table). Only one gene, a putative heat shock protein (Glyma09g24410), was down-regulated in response to elevated VPD in both of the slow-wilting genotypes. The ANK (Gm08:6264434—‐6265362) differentially expressed in Hutcheson was also down-regulated in PI416937 in response to increased VPD. Analyses of gene ontology (GO) [12] revealed that many biological processes and cellular and molecular components were enriched in response to changes in VPD in PI 416937 (Fig 4, S4 Table and S3 Fig). Overall, 577 transcripts in the significantly regulated list of 944 transcripts, and 21,058 transcripts from all 39,068 expressed transcripts were annotated using GO analysis. The GO terms represented among all expressed transcripts was compared to GO terms represented among all differentially expressed transcripts using the SEA method. Fig 4 identifies GO terms significantly enriched among differentially regulated genes compared to the all expressed genes. Major biological processes such as carbohydrate metabolism, transcription regulation, lipid metabolism, oxidative stress response, cell wall organization, and cell wall modification genes were enriched in PI 416937 under high VPD conditions. For molecular functions, catalytic activity genes, transferases, hydrolases, heme-binding motifs, transcription factors, peptidases, peroxidases and antioxidants were over-represented. Under high VPD, cellular components, apoplast, microtubule skeleton and β-galactosidase complexes were over-represented (Fig 4). Under all categories, genes related to cell wall organization were preferentially regulated, which indicated that cell wall-related processes may be modified under high VPD conditions in PI 416937.


Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean.

Devi MJ, Sinclair TR, Taliercio E - PLoS ONE (2015)

GO terms significantly over-represented (higher percentage) in the differentially expressed genes list compared to background/reference genes (all expressed genes) list in PI 416937 under high VPD conditions.Green bars represent percentage of genes belongs to GO categories from total number of up- and down-regulated genes due to high VPD, and red bars indicate percentage of genes belong to GO category from total number of background/reference list in PI 416937.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591296&req=5

pone.0139134.g004: GO terms significantly over-represented (higher percentage) in the differentially expressed genes list compared to background/reference genes (all expressed genes) list in PI 416937 under high VPD conditions.Green bars represent percentage of genes belongs to GO categories from total number of up- and down-regulated genes due to high VPD, and red bars indicate percentage of genes belong to GO category from total number of background/reference list in PI 416937.
Mentions: In contrast to PI 471938 and Hutcheson, genotype PI 416937 differentially regulated 944 genes when VPD was elevated. Of the 944 differentially regulated genes, 425 were up-regulated and 519 genes were down-regulated (Fig 2 and S3 Table). Only one gene, a putative heat shock protein (Glyma09g24410), was down-regulated in response to elevated VPD in both of the slow-wilting genotypes. The ANK (Gm08:6264434—‐6265362) differentially expressed in Hutcheson was also down-regulated in PI416937 in response to increased VPD. Analyses of gene ontology (GO) [12] revealed that many biological processes and cellular and molecular components were enriched in response to changes in VPD in PI 416937 (Fig 4, S4 Table and S3 Fig). Overall, 577 transcripts in the significantly regulated list of 944 transcripts, and 21,058 transcripts from all 39,068 expressed transcripts were annotated using GO analysis. The GO terms represented among all expressed transcripts was compared to GO terms represented among all differentially expressed transcripts using the SEA method. Fig 4 identifies GO terms significantly enriched among differentially regulated genes compared to the all expressed genes. Major biological processes such as carbohydrate metabolism, transcription regulation, lipid metabolism, oxidative stress response, cell wall organization, and cell wall modification genes were enriched in PI 416937 under high VPD conditions. For molecular functions, catalytic activity genes, transferases, hydrolases, heme-binding motifs, transcription factors, peptidases, peroxidases and antioxidants were over-represented. Under high VPD, cellular components, apoplast, microtubule skeleton and β-galactosidase complexes were over-represented (Fig 4). Under all categories, genes related to cell wall organization were preferentially regulated, which indicated that cell wall-related processes may be modified under high VPD conditions in PI 416937.

Bottom Line: The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes.TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa.The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes. Hutcheson, a fast-wilting soybean, and PI 471938, a slow-wilting soybean, respond to increased VPD with a linear increase in TR. TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa. The objective of this study was to identify the response of the transcriptome of these accessions to elevated VPD under well-watered conditions and identify responses that are unique to the slow-wilting accessions. Gene expression analysis in leaves of genotypes PI 471938 and Hutcheson showed that 22 and 1 genes, respectively, were differentially expressed under high VPD. In contrast, there were 944 genes differentially expressed in PI 416937 with the same increase in VPD. The increased alteration of the transcriptome of PI 416937 in response to elevated VPD clearly distinguished it from the other slow-wilting PI 471938 and the fast-wilting Hutcheson. The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

No MeSH data available.