Limits...
Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean.

Devi MJ, Sinclair TR, Taliercio E - PLoS ONE (2015)

Bottom Line: The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes.TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa.The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes. Hutcheson, a fast-wilting soybean, and PI 471938, a slow-wilting soybean, respond to increased VPD with a linear increase in TR. TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa. The objective of this study was to identify the response of the transcriptome of these accessions to elevated VPD under well-watered conditions and identify responses that are unique to the slow-wilting accessions. Gene expression analysis in leaves of genotypes PI 471938 and Hutcheson showed that 22 and 1 genes, respectively, were differentially expressed under high VPD. In contrast, there were 944 genes differentially expressed in PI 416937 with the same increase in VPD. The increased alteration of the transcriptome of PI 416937 in response to elevated VPD clearly distinguished it from the other slow-wilting PI 471938 and the fast-wilting Hutcheson. The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

No MeSH data available.


Number of genes differentially expressed in leaves of Hutcheson, PI 471938 and PI 416937 exposed to high VPD.Black bars represent number of genes up-regulated in each genotype, and grey bars correspond to number of down-regulated genes.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4591296&req=5

pone.0139134.g002: Number of genes differentially expressed in leaves of Hutcheson, PI 471938 and PI 416937 exposed to high VPD.Black bars represent number of genes up-regulated in each genotype, and grey bars correspond to number of down-regulated genes.

Mentions: RNA was isolated from the second leaf from the apical meristem from plants of all three genotypes that had been exposed to a low VPD environment (0.7 to 1.2 kPa) or challenged by a VPD above 2 kPa (3 to 3.5 kPa). We compared gene expression within the genotypes and between the treatments to understand the differences in the TR responses of the soybean genotypes to high VPD conditions. These comparisons identified the genes differentially regulated under elevated VPD conditions in the three genotypes. A total of 39,068 genes were expressed in leaves of soybean plants exposed to low or high VPD environments. The high total number of expressed genes probably reflects the fact that genes expressed in any genotype with low levels of expression in both treatments were included. To identify the gene expression pattern between the treatments, the numbers of up-regulated and down-regulated genes were calculated for high VPD samples in comparison to low VPD samples. The fast-wilting Hutcheson and the slow-wilting PI 471938 transcriptomes were fairly unresponsive to an increase in VPD with significantly (q<0.05) altered expression of only 1 and 22 transcripts, respectively (Fig 2 and Table 2). The transcriptome of PI 416937 was markedly more responsive to a comparable increase in VPD with a significant change in expression of over 900 transcripts (p<0.05) (Fig 2 and S3 Table).


Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean.

Devi MJ, Sinclair TR, Taliercio E - PLoS ONE (2015)

Number of genes differentially expressed in leaves of Hutcheson, PI 471938 and PI 416937 exposed to high VPD.Black bars represent number of genes up-regulated in each genotype, and grey bars correspond to number of down-regulated genes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591296&req=5

pone.0139134.g002: Number of genes differentially expressed in leaves of Hutcheson, PI 471938 and PI 416937 exposed to high VPD.Black bars represent number of genes up-regulated in each genotype, and grey bars correspond to number of down-regulated genes.
Mentions: RNA was isolated from the second leaf from the apical meristem from plants of all three genotypes that had been exposed to a low VPD environment (0.7 to 1.2 kPa) or challenged by a VPD above 2 kPa (3 to 3.5 kPa). We compared gene expression within the genotypes and between the treatments to understand the differences in the TR responses of the soybean genotypes to high VPD conditions. These comparisons identified the genes differentially regulated under elevated VPD conditions in the three genotypes. A total of 39,068 genes were expressed in leaves of soybean plants exposed to low or high VPD environments. The high total number of expressed genes probably reflects the fact that genes expressed in any genotype with low levels of expression in both treatments were included. To identify the gene expression pattern between the treatments, the numbers of up-regulated and down-regulated genes were calculated for high VPD samples in comparison to low VPD samples. The fast-wilting Hutcheson and the slow-wilting PI 471938 transcriptomes were fairly unresponsive to an increase in VPD with significantly (q<0.05) altered expression of only 1 and 22 transcripts, respectively (Fig 2 and Table 2). The transcriptome of PI 416937 was markedly more responsive to a comparable increase in VPD with a significant change in expression of over 900 transcripts (p<0.05) (Fig 2 and S3 Table).

Bottom Line: The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes.TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa.The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes. Hutcheson, a fast-wilting soybean, and PI 471938, a slow-wilting soybean, respond to increased VPD with a linear increase in TR. TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa. The objective of this study was to identify the response of the transcriptome of these accessions to elevated VPD under well-watered conditions and identify responses that are unique to the slow-wilting accessions. Gene expression analysis in leaves of genotypes PI 471938 and Hutcheson showed that 22 and 1 genes, respectively, were differentially expressed under high VPD. In contrast, there were 944 genes differentially expressed in PI 416937 with the same increase in VPD. The increased alteration of the transcriptome of PI 416937 in response to elevated VPD clearly distinguished it from the other slow-wilting PI 471938 and the fast-wilting Hutcheson. The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

No MeSH data available.