Limits...
Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean.

Devi MJ, Sinclair TR, Taliercio E - PLoS ONE (2015)

Bottom Line: The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes.TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa.The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes. Hutcheson, a fast-wilting soybean, and PI 471938, a slow-wilting soybean, respond to increased VPD with a linear increase in TR. TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa. The objective of this study was to identify the response of the transcriptome of these accessions to elevated VPD under well-watered conditions and identify responses that are unique to the slow-wilting accessions. Gene expression analysis in leaves of genotypes PI 471938 and Hutcheson showed that 22 and 1 genes, respectively, were differentially expressed under high VPD. In contrast, there were 944 genes differentially expressed in PI 416937 with the same increase in VPD. The increased alteration of the transcriptome of PI 416937 in response to elevated VPD clearly distinguished it from the other slow-wilting PI 471938 and the fast-wilting Hutcheson. The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

No MeSH data available.


Related in: MedlinePlus

Transpiration rate (TR) response of (a) Hutcheson (b) PI 471938 and (c) PI 416938 to low, medium and high vapor pressure deficit (VPD) conditions from experiments 1 and 2 (4 replicates in each experiment at each VPD level).X-axis represents VPD levels, and the Y-axis represents corresponding transpiration rates. Closed, half closed and open circles are TR values under low, medium and high VPD conditions, respectively. Data points are the individual plant TRs exposed to various VPDs. The regressions in (a) and (b) are single linear relationships, and the regression in (c) is a two-segmental nonlinear relationship. More details are shown in S2 Table.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4591296&req=5

pone.0139134.g001: Transpiration rate (TR) response of (a) Hutcheson (b) PI 471938 and (c) PI 416938 to low, medium and high vapor pressure deficit (VPD) conditions from experiments 1 and 2 (4 replicates in each experiment at each VPD level).X-axis represents VPD levels, and the Y-axis represents corresponding transpiration rates. Closed, half closed and open circles are TR values under low, medium and high VPD conditions, respectively. Data points are the individual plant TRs exposed to various VPDs. The regressions in (a) and (b) are single linear relationships, and the regression in (c) is a two-segmental nonlinear relationship. More details are shown in S2 Table.

Mentions: Among the three genotypes, only PI 416937 showed the limited or altered TR response to VPD. The response of PI 416937 was best described by a two-segment regression model with a VPD break point at about 2 kPa VPD (Fig 1). By contrast, TR of the genotypes PI 471938 and Hutcheson displayed the typical linear response of soybean over the entire range of VPD conditions, as has been reported previously [5,7].


Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean.

Devi MJ, Sinclair TR, Taliercio E - PLoS ONE (2015)

Transpiration rate (TR) response of (a) Hutcheson (b) PI 471938 and (c) PI 416938 to low, medium and high vapor pressure deficit (VPD) conditions from experiments 1 and 2 (4 replicates in each experiment at each VPD level).X-axis represents VPD levels, and the Y-axis represents corresponding transpiration rates. Closed, half closed and open circles are TR values under low, medium and high VPD conditions, respectively. Data points are the individual plant TRs exposed to various VPDs. The regressions in (a) and (b) are single linear relationships, and the regression in (c) is a two-segmental nonlinear relationship. More details are shown in S2 Table.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591296&req=5

pone.0139134.g001: Transpiration rate (TR) response of (a) Hutcheson (b) PI 471938 and (c) PI 416938 to low, medium and high vapor pressure deficit (VPD) conditions from experiments 1 and 2 (4 replicates in each experiment at each VPD level).X-axis represents VPD levels, and the Y-axis represents corresponding transpiration rates. Closed, half closed and open circles are TR values under low, medium and high VPD conditions, respectively. Data points are the individual plant TRs exposed to various VPDs. The regressions in (a) and (b) are single linear relationships, and the regression in (c) is a two-segmental nonlinear relationship. More details are shown in S2 Table.
Mentions: Among the three genotypes, only PI 416937 showed the limited or altered TR response to VPD. The response of PI 416937 was best described by a two-segment regression model with a VPD break point at about 2 kPa VPD (Fig 1). By contrast, TR of the genotypes PI 471938 and Hutcheson displayed the typical linear response of soybean over the entire range of VPD conditions, as has been reported previously [5,7].

Bottom Line: The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes.TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa.The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, North Carolina State University, Raleigh, North Carolina, United States of America.

ABSTRACT
Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes. Hutcheson, a fast-wilting soybean, and PI 471938, a slow-wilting soybean, respond to increased VPD with a linear increase in TR. TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa. The objective of this study was to identify the response of the transcriptome of these accessions to elevated VPD under well-watered conditions and identify responses that are unique to the slow-wilting accessions. Gene expression analysis in leaves of genotypes PI 471938 and Hutcheson showed that 22 and 1 genes, respectively, were differentially expressed under high VPD. In contrast, there were 944 genes differentially expressed in PI 416937 with the same increase in VPD. The increased alteration of the transcriptome of PI 416937 in response to elevated VPD clearly distinguished it from the other slow-wilting PI 471938 and the fast-wilting Hutcheson. The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.

No MeSH data available.


Related in: MedlinePlus