Limits...
Fluorescence-Guided Surgery of Liver Metastasis in Orthotopic Nude-Mouse Models.

Murakami T, Hiroshima Y, Zhang Y, Chishima T, Tanaka K, Bouvet M, Endo I, Hoffman RM - PLoS ONE (2015)

Bottom Line: Post-surgical residual tumor fluorescence was visualized with the OV100 Small Animal Imaging System.Residual tumor fluorescence after BLS was clearly visualized at high magnification with the OV100.In contrast, residual tumor fluorescence after FGS was not detected even at high magnification with the OV100.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer, Inc., San Diego, California, United States of America; Department of Surgery, University of California San Diego, San Diego, California, United States of America; Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.

ABSTRACT
We report here the development of fluorescence-guided surgery of liver metastasis. HT29 human colon cancer cells expressing green fluorescent protein (GFP) were initially injected in the spleen of nude mice. Three weeks later, established liver metastases were harvested and implanted on the left lobe of the liver in other nude mice in order to make an orthotopic liver metastasis model. Fourteen mice with a single liver metastasis were randomized into bright-light surgery (BLS) or fluorescence-guided surgery (FGS) groups. Seven mice were treated with BLS, seven were treated with FGS. Three weeks after implantation, the left lobe of the liver with a single metastasis was exposed through a median abdominal incision. BLS was performed under white light. FGS was performed using a hand-held portable fluorescence imaging system (Dino-Lite). Post-surgical residual tumor fluorescence was visualized with the OV100 Small Animal Imaging System. Residual tumor fluorescence after BLS was clearly visualized at high magnification with the OV100. In contrast, residual tumor fluorescence after FGS was not detected even at high magnification with the OV100. These results demonstrate the feasibility of FGS for liver metastasis.

No MeSH data available.


Related in: MedlinePlus

Schematic diagram of the experimental design.(A) Fourteen mice were randomized into 2 groups; FGS: n = 7, BLS: n = 7. (B) Timeline from orthotopic implantation. Four weeks after implantation, all mice were treated with FGS or BLS. Twenty-eight days after the surgery, all mice underwent laparotomy to detect GFP for evaluation of recurrence. After the laparotomy, follow-up examination for survival was continued. (FGS, fluorescence-guided surgery; BLS, bright-light surgery).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4591295&req=5

pone.0138752.g002: Schematic diagram of the experimental design.(A) Fourteen mice were randomized into 2 groups; FGS: n = 7, BLS: n = 7. (B) Timeline from orthotopic implantation. Four weeks after implantation, all mice were treated with FGS or BLS. Twenty-eight days after the surgery, all mice underwent laparotomy to detect GFP for evaluation of recurrence. After the laparotomy, follow-up examination for survival was continued. (FGS, fluorescence-guided surgery; BLS, bright-light surgery).

Mentions: Four weeks after SOI of HT-29-GFP to the liver, the liver metastasis was exposed and imaged preoperatively with the OV100 Small Animal Imaging System (Olympus, Tokyo, Japan) [16] at a magnification of 0.14x. Fourteen mice underwent surgery: Fluorescence-guided surgery (FGS) in 7 mice and bright-light surgery (BLS) in 7 mice (Fig 2A and 2B). FGS was performed using the Dino-Lite imaging system (AM4113TGFBW Dino-Lite Premier; AnMo Electronics Corporation, New Taiwan). The surgical resection bed was imaged with the Olympus OV100 at a magnification of 0.14x or 0.56x to detect microscopic residual cancer. Residual tumor area was analyzed with ImageJ v1.49f (National Institutes of Health). The incision was closed in one layer using 6–0 nylon surgical sutures.


Fluorescence-Guided Surgery of Liver Metastasis in Orthotopic Nude-Mouse Models.

Murakami T, Hiroshima Y, Zhang Y, Chishima T, Tanaka K, Bouvet M, Endo I, Hoffman RM - PLoS ONE (2015)

Schematic diagram of the experimental design.(A) Fourteen mice were randomized into 2 groups; FGS: n = 7, BLS: n = 7. (B) Timeline from orthotopic implantation. Four weeks after implantation, all mice were treated with FGS or BLS. Twenty-eight days after the surgery, all mice underwent laparotomy to detect GFP for evaluation of recurrence. After the laparotomy, follow-up examination for survival was continued. (FGS, fluorescence-guided surgery; BLS, bright-light surgery).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591295&req=5

pone.0138752.g002: Schematic diagram of the experimental design.(A) Fourteen mice were randomized into 2 groups; FGS: n = 7, BLS: n = 7. (B) Timeline from orthotopic implantation. Four weeks after implantation, all mice were treated with FGS or BLS. Twenty-eight days after the surgery, all mice underwent laparotomy to detect GFP for evaluation of recurrence. After the laparotomy, follow-up examination for survival was continued. (FGS, fluorescence-guided surgery; BLS, bright-light surgery).
Mentions: Four weeks after SOI of HT-29-GFP to the liver, the liver metastasis was exposed and imaged preoperatively with the OV100 Small Animal Imaging System (Olympus, Tokyo, Japan) [16] at a magnification of 0.14x. Fourteen mice underwent surgery: Fluorescence-guided surgery (FGS) in 7 mice and bright-light surgery (BLS) in 7 mice (Fig 2A and 2B). FGS was performed using the Dino-Lite imaging system (AM4113TGFBW Dino-Lite Premier; AnMo Electronics Corporation, New Taiwan). The surgical resection bed was imaged with the Olympus OV100 at a magnification of 0.14x or 0.56x to detect microscopic residual cancer. Residual tumor area was analyzed with ImageJ v1.49f (National Institutes of Health). The incision was closed in one layer using 6–0 nylon surgical sutures.

Bottom Line: Post-surgical residual tumor fluorescence was visualized with the OV100 Small Animal Imaging System.Residual tumor fluorescence after BLS was clearly visualized at high magnification with the OV100.In contrast, residual tumor fluorescence after FGS was not detected even at high magnification with the OV100.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer, Inc., San Diego, California, United States of America; Department of Surgery, University of California San Diego, San Diego, California, United States of America; Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.

ABSTRACT
We report here the development of fluorescence-guided surgery of liver metastasis. HT29 human colon cancer cells expressing green fluorescent protein (GFP) were initially injected in the spleen of nude mice. Three weeks later, established liver metastases were harvested and implanted on the left lobe of the liver in other nude mice in order to make an orthotopic liver metastasis model. Fourteen mice with a single liver metastasis were randomized into bright-light surgery (BLS) or fluorescence-guided surgery (FGS) groups. Seven mice were treated with BLS, seven were treated with FGS. Three weeks after implantation, the left lobe of the liver with a single metastasis was exposed through a median abdominal incision. BLS was performed under white light. FGS was performed using a hand-held portable fluorescence imaging system (Dino-Lite). Post-surgical residual tumor fluorescence was visualized with the OV100 Small Animal Imaging System. Residual tumor fluorescence after BLS was clearly visualized at high magnification with the OV100. In contrast, residual tumor fluorescence after FGS was not detected even at high magnification with the OV100. These results demonstrate the feasibility of FGS for liver metastasis.

No MeSH data available.


Related in: MedlinePlus