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Fluorescence-Guided Surgery of Liver Metastasis in Orthotopic Nude-Mouse Models.

Murakami T, Hiroshima Y, Zhang Y, Chishima T, Tanaka K, Bouvet M, Endo I, Hoffman RM - PLoS ONE (2015)

Bottom Line: Post-surgical residual tumor fluorescence was visualized with the OV100 Small Animal Imaging System.Residual tumor fluorescence after BLS was clearly visualized at high magnification with the OV100.In contrast, residual tumor fluorescence after FGS was not detected even at high magnification with the OV100.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer, Inc., San Diego, California, United States of America; Department of Surgery, University of California San Diego, San Diego, California, United States of America; Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.

ABSTRACT
We report here the development of fluorescence-guided surgery of liver metastasis. HT29 human colon cancer cells expressing green fluorescent protein (GFP) were initially injected in the spleen of nude mice. Three weeks later, established liver metastases were harvested and implanted on the left lobe of the liver in other nude mice in order to make an orthotopic liver metastasis model. Fourteen mice with a single liver metastasis were randomized into bright-light surgery (BLS) or fluorescence-guided surgery (FGS) groups. Seven mice were treated with BLS, seven were treated with FGS. Three weeks after implantation, the left lobe of the liver with a single metastasis was exposed through a median abdominal incision. BLS was performed under white light. FGS was performed using a hand-held portable fluorescence imaging system (Dino-Lite). Post-surgical residual tumor fluorescence was visualized with the OV100 Small Animal Imaging System. Residual tumor fluorescence after BLS was clearly visualized at high magnification with the OV100. In contrast, residual tumor fluorescence after FGS was not detected even at high magnification with the OV100. These results demonstrate the feasibility of FGS for liver metastasis.

No MeSH data available.


Related in: MedlinePlus

HT29-GFP colon cancer experimental liver metastasis.(A) HT-29-GFP cells (5×105 in 50 μl with 50% Matrigel) were injected into the superior pole and inferior pole of the spleen (arrows), respectively. (B) Three weeks after injection, liver metastasis was confirmed by laparotomy, which was resected and cut into 3-mm3 blocks. Each tumor fragment was implanted by surgical orthotopic implantation (SOI) in the left lobe of the liver on other nude mice. (C) Representative images of liver metastasis established after spleen injection. The large tumor in the liver strongly expressed GFP. (GFP, green fluorescent protein; BF, bright field)
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pone.0138752.g001: HT29-GFP colon cancer experimental liver metastasis.(A) HT-29-GFP cells (5×105 in 50 μl with 50% Matrigel) were injected into the superior pole and inferior pole of the spleen (arrows), respectively. (B) Three weeks after injection, liver metastasis was confirmed by laparotomy, which was resected and cut into 3-mm3 blocks. Each tumor fragment was implanted by surgical orthotopic implantation (SOI) in the left lobe of the liver on other nude mice. (C) Representative images of liver metastasis established after spleen injection. The large tumor in the liver strongly expressed GFP. (GFP, green fluorescent protein; BF, bright field)

Mentions: HT-29-GFP cells were harvested by trypsinization and washed twice with serum-free medium. Cells (5×105 in 50 μl serum-free medium with 50% Matrigel) were injected into the superior and inferior pole of the spleen in mice (Fig 1A). Three week after injection, liver metastasis was confirmed by laparotomy (Fig 1C).


Fluorescence-Guided Surgery of Liver Metastasis in Orthotopic Nude-Mouse Models.

Murakami T, Hiroshima Y, Zhang Y, Chishima T, Tanaka K, Bouvet M, Endo I, Hoffman RM - PLoS ONE (2015)

HT29-GFP colon cancer experimental liver metastasis.(A) HT-29-GFP cells (5×105 in 50 μl with 50% Matrigel) were injected into the superior pole and inferior pole of the spleen (arrows), respectively. (B) Three weeks after injection, liver metastasis was confirmed by laparotomy, which was resected and cut into 3-mm3 blocks. Each tumor fragment was implanted by surgical orthotopic implantation (SOI) in the left lobe of the liver on other nude mice. (C) Representative images of liver metastasis established after spleen injection. The large tumor in the liver strongly expressed GFP. (GFP, green fluorescent protein; BF, bright field)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591295&req=5

pone.0138752.g001: HT29-GFP colon cancer experimental liver metastasis.(A) HT-29-GFP cells (5×105 in 50 μl with 50% Matrigel) were injected into the superior pole and inferior pole of the spleen (arrows), respectively. (B) Three weeks after injection, liver metastasis was confirmed by laparotomy, which was resected and cut into 3-mm3 blocks. Each tumor fragment was implanted by surgical orthotopic implantation (SOI) in the left lobe of the liver on other nude mice. (C) Representative images of liver metastasis established after spleen injection. The large tumor in the liver strongly expressed GFP. (GFP, green fluorescent protein; BF, bright field)
Mentions: HT-29-GFP cells were harvested by trypsinization and washed twice with serum-free medium. Cells (5×105 in 50 μl serum-free medium with 50% Matrigel) were injected into the superior and inferior pole of the spleen in mice (Fig 1A). Three week after injection, liver metastasis was confirmed by laparotomy (Fig 1C).

Bottom Line: Post-surgical residual tumor fluorescence was visualized with the OV100 Small Animal Imaging System.Residual tumor fluorescence after BLS was clearly visualized at high magnification with the OV100.In contrast, residual tumor fluorescence after FGS was not detected even at high magnification with the OV100.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer, Inc., San Diego, California, United States of America; Department of Surgery, University of California San Diego, San Diego, California, United States of America; Department of Gastroenterological Surgery, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.

ABSTRACT
We report here the development of fluorescence-guided surgery of liver metastasis. HT29 human colon cancer cells expressing green fluorescent protein (GFP) were initially injected in the spleen of nude mice. Three weeks later, established liver metastases were harvested and implanted on the left lobe of the liver in other nude mice in order to make an orthotopic liver metastasis model. Fourteen mice with a single liver metastasis were randomized into bright-light surgery (BLS) or fluorescence-guided surgery (FGS) groups. Seven mice were treated with BLS, seven were treated with FGS. Three weeks after implantation, the left lobe of the liver with a single metastasis was exposed through a median abdominal incision. BLS was performed under white light. FGS was performed using a hand-held portable fluorescence imaging system (Dino-Lite). Post-surgical residual tumor fluorescence was visualized with the OV100 Small Animal Imaging System. Residual tumor fluorescence after BLS was clearly visualized at high magnification with the OV100. In contrast, residual tumor fluorescence after FGS was not detected even at high magnification with the OV100. These results demonstrate the feasibility of FGS for liver metastasis.

No MeSH data available.


Related in: MedlinePlus