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Postoperative Changes in Aqueous Monocyte Chemotactic Protein-1 Levels and Bleb Morphology after Trabeculectomy vs. Ex-PRESS Shunt Surgery.

Shobayashi K, Inoue T, Kawai M, Iwao K, Ohira S, Kojima S, Kuroda U, Nakashima K, Tanihara H - PLoS ONE (2015)

Bottom Line: In the Ex-PRESS group, the corresponding values were 1744.0, 1372.0, 932.5, 711.7, 396.1, 487.3, 799.5, 1327.9, 293.6, and 184.0 pg/mL.There were no significant differences in the aqueous MCP-1 level between the groups at any time point.The postoperative aqueous MCP-1 level had bimodal peaks in both groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.

ABSTRACT

Purpose: To evaluate the postoperative changes in blebs and levels of aqueous monocyte chemotactic protein-1 (MCP-1) after trabeculectomy vs. Ex-PRESS tube shunt surgery.

Methods: Rabbits were subjected to trabeculectomy or Ex-PRESS tube shunt surgery and observed for up to 3 months. Intraocular pressure (IOP) was measured using a rebound tonometer. The MCP-1 level was measured by enzyme-linked immunosorbent assay (ELISA). Bleb morphology was evaluated using photos and anterior-segment optical coherence tomography (OCT).

Results: There were no differences in bleb appearance or IOP at any time between the groups. Bleb wall density in the anterior-segment OCT image was significantly lower 1 week after surgery in the Ex-PRESS group than the trabeculectomy group. The MCP-1 level in control eyes was 304.1 ± 45.2 pg/mL. In the trabeculectomy group, the mean aqueous MCP-1 level was 1444.9, 1914.3, 1899.8, 516.4, 398.3, 427.3, 609.5, 1612.7, 386.2, and 167.9 pg/mL at 3, 6, and 12 h, and 1, 2, 5, 7, 14, 30, and 90 days after surgery, respectively. In the Ex-PRESS group, the corresponding values were 1744.0, 1372.0, 932.5, 711.7, 396.1, 487.3, 799.5, 1327.9, 293.6, and 184.0 pg/mL. There were no significant differences in the aqueous MCP-1 level between the groups at any time point.

Conclusion: The postoperative changes were similar in the Ex-PRESS and trabeculectomy groups, except for bleb wall density in the anterior-segment OCT image. The postoperative aqueous MCP-1 level had bimodal peaks in both groups.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical analysis.α-SMA-positive cells were detected as brown cells in the bleb after trabeculectomy (A, C) and Ex-PRESS tube shunt surgery (B, D). The red squares in A and B outline C and D (higher magnification), respectively. Scale bar, 1 mm (A, B) and 200 μm (C, D).
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pone.0139751.g004: Immunohistochemical analysis.α-SMA-positive cells were detected as brown cells in the bleb after trabeculectomy (A, C) and Ex-PRESS tube shunt surgery (B, D). The red squares in A and B outline C and D (higher magnification), respectively. Scale bar, 1 mm (A, B) and 200 μm (C, D).

Mentions: Generally, the amount of α-SMA positive cells represents the extent of tissue scarring at the time point. The immunohistochemical analysis showed immunoreactivity for α-SMA in cells around the fluid cavity in the bleb, suggesting that the conjunctiva cells partially transdifferentiated into myofibroblast-like cells (Fig 4). Qualitatively, there were no significant differences in the distribution of α-SMA-positive cells between the trabeculectomy and Ex-PRESS groups.


Postoperative Changes in Aqueous Monocyte Chemotactic Protein-1 Levels and Bleb Morphology after Trabeculectomy vs. Ex-PRESS Shunt Surgery.

Shobayashi K, Inoue T, Kawai M, Iwao K, Ohira S, Kojima S, Kuroda U, Nakashima K, Tanihara H - PLoS ONE (2015)

Immunohistochemical analysis.α-SMA-positive cells were detected as brown cells in the bleb after trabeculectomy (A, C) and Ex-PRESS tube shunt surgery (B, D). The red squares in A and B outline C and D (higher magnification), respectively. Scale bar, 1 mm (A, B) and 200 μm (C, D).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591270&req=5

pone.0139751.g004: Immunohistochemical analysis.α-SMA-positive cells were detected as brown cells in the bleb after trabeculectomy (A, C) and Ex-PRESS tube shunt surgery (B, D). The red squares in A and B outline C and D (higher magnification), respectively. Scale bar, 1 mm (A, B) and 200 μm (C, D).
Mentions: Generally, the amount of α-SMA positive cells represents the extent of tissue scarring at the time point. The immunohistochemical analysis showed immunoreactivity for α-SMA in cells around the fluid cavity in the bleb, suggesting that the conjunctiva cells partially transdifferentiated into myofibroblast-like cells (Fig 4). Qualitatively, there were no significant differences in the distribution of α-SMA-positive cells between the trabeculectomy and Ex-PRESS groups.

Bottom Line: In the Ex-PRESS group, the corresponding values were 1744.0, 1372.0, 932.5, 711.7, 396.1, 487.3, 799.5, 1327.9, 293.6, and 184.0 pg/mL.There were no significant differences in the aqueous MCP-1 level between the groups at any time point.The postoperative aqueous MCP-1 level had bimodal peaks in both groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.

ABSTRACT

Purpose: To evaluate the postoperative changes in blebs and levels of aqueous monocyte chemotactic protein-1 (MCP-1) after trabeculectomy vs. Ex-PRESS tube shunt surgery.

Methods: Rabbits were subjected to trabeculectomy or Ex-PRESS tube shunt surgery and observed for up to 3 months. Intraocular pressure (IOP) was measured using a rebound tonometer. The MCP-1 level was measured by enzyme-linked immunosorbent assay (ELISA). Bleb morphology was evaluated using photos and anterior-segment optical coherence tomography (OCT).

Results: There were no differences in bleb appearance or IOP at any time between the groups. Bleb wall density in the anterior-segment OCT image was significantly lower 1 week after surgery in the Ex-PRESS group than the trabeculectomy group. The MCP-1 level in control eyes was 304.1 ± 45.2 pg/mL. In the trabeculectomy group, the mean aqueous MCP-1 level was 1444.9, 1914.3, 1899.8, 516.4, 398.3, 427.3, 609.5, 1612.7, 386.2, and 167.9 pg/mL at 3, 6, and 12 h, and 1, 2, 5, 7, 14, 30, and 90 days after surgery, respectively. In the Ex-PRESS group, the corresponding values were 1744.0, 1372.0, 932.5, 711.7, 396.1, 487.3, 799.5, 1327.9, 293.6, and 184.0 pg/mL. There were no significant differences in the aqueous MCP-1 level between the groups at any time point.

Conclusion: The postoperative changes were similar in the Ex-PRESS and trabeculectomy groups, except for bleb wall density in the anterior-segment OCT image. The postoperative aqueous MCP-1 level had bimodal peaks in both groups.

No MeSH data available.


Related in: MedlinePlus