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Postoperative Changes in Aqueous Monocyte Chemotactic Protein-1 Levels and Bleb Morphology after Trabeculectomy vs. Ex-PRESS Shunt Surgery.

Shobayashi K, Inoue T, Kawai M, Iwao K, Ohira S, Kojima S, Kuroda U, Nakashima K, Tanihara H - PLoS ONE (2015)

Bottom Line: In the Ex-PRESS group, the corresponding values were 1744.0, 1372.0, 932.5, 711.7, 396.1, 487.3, 799.5, 1327.9, 293.6, and 184.0 pg/mL.There were no significant differences in the aqueous MCP-1 level between the groups at any time point.The postoperative aqueous MCP-1 level had bimodal peaks in both groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.

ABSTRACT

Purpose: To evaluate the postoperative changes in blebs and levels of aqueous monocyte chemotactic protein-1 (MCP-1) after trabeculectomy vs. Ex-PRESS tube shunt surgery.

Methods: Rabbits were subjected to trabeculectomy or Ex-PRESS tube shunt surgery and observed for up to 3 months. Intraocular pressure (IOP) was measured using a rebound tonometer. The MCP-1 level was measured by enzyme-linked immunosorbent assay (ELISA). Bleb morphology was evaluated using photos and anterior-segment optical coherence tomography (OCT).

Results: There were no differences in bleb appearance or IOP at any time between the groups. Bleb wall density in the anterior-segment OCT image was significantly lower 1 week after surgery in the Ex-PRESS group than the trabeculectomy group. The MCP-1 level in control eyes was 304.1 ± 45.2 pg/mL. In the trabeculectomy group, the mean aqueous MCP-1 level was 1444.9, 1914.3, 1899.8, 516.4, 398.3, 427.3, 609.5, 1612.7, 386.2, and 167.9 pg/mL at 3, 6, and 12 h, and 1, 2, 5, 7, 14, 30, and 90 days after surgery, respectively. In the Ex-PRESS group, the corresponding values were 1744.0, 1372.0, 932.5, 711.7, 396.1, 487.3, 799.5, 1327.9, 293.6, and 184.0 pg/mL. There were no significant differences in the aqueous MCP-1 level between the groups at any time point.

Conclusion: The postoperative changes were similar in the Ex-PRESS and trabeculectomy groups, except for bleb wall density in the anterior-segment OCT image. The postoperative aqueous MCP-1 level had bimodal peaks in both groups.

No MeSH data available.


Related in: MedlinePlus

Intensity of the bleb wall measured using anterior-segment optical coherence tomography.Representative images in a horizontal scan 1 week after trabeculectomy (A) and Ex-PRESS tube shunt surgery (B). Time course of intensity. Open circles with dotted line, trabeculectomy group; closed circles with solid line, Ex-PRESS tube shunt surgery group. Mean ± standard error. *P < 0.05 by Wilcoxon rank-sum test.
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pone.0139751.g003: Intensity of the bleb wall measured using anterior-segment optical coherence tomography.Representative images in a horizontal scan 1 week after trabeculectomy (A) and Ex-PRESS tube shunt surgery (B). Time course of intensity. Open circles with dotted line, trabeculectomy group; closed circles with solid line, Ex-PRESS tube shunt surgery group. Mean ± standard error. *P < 0.05 by Wilcoxon rank-sum test.

Mentions: Representative blebs are shown in Fig 2A. Similar avascular cystic blebs were observed in the groups. In both groups, the bleb became flat by 3 months after surgery. There were no significant differences in area or vascularity between the groups at any time point after surgery (Fig 2B and 2C). In all eyes after shunt surgery, the device remained attached to the iris at all time points. In the AS-OCT images (Fig 3), only 3 (12.5%) of 24 eyes had a fluid-filled space, and many of the blebs were filled with sponge-like tissue, followed by a vague shadow of the scleral bed. This made it difficult to compare the imaging characteristics (i.e., height and wall thickness of the bleb, height of the fluid-filled cavity, filtration opening in the scleral flap) between the groups. However, the density of the bleb wall was significantly lower 1 week after surgery in the Ex-PRESS group than the trabeculectomy group (P < 0.05). Two days after surgery it was significantly denser than 7 and 14 days postoperatively in the Ex-PRESS group and at 14 days in the trabeculectomy group, suggesting a more rapid decrease in the Ex-PRESS group than in the trabeculectomy group.


Postoperative Changes in Aqueous Monocyte Chemotactic Protein-1 Levels and Bleb Morphology after Trabeculectomy vs. Ex-PRESS Shunt Surgery.

Shobayashi K, Inoue T, Kawai M, Iwao K, Ohira S, Kojima S, Kuroda U, Nakashima K, Tanihara H - PLoS ONE (2015)

Intensity of the bleb wall measured using anterior-segment optical coherence tomography.Representative images in a horizontal scan 1 week after trabeculectomy (A) and Ex-PRESS tube shunt surgery (B). Time course of intensity. Open circles with dotted line, trabeculectomy group; closed circles with solid line, Ex-PRESS tube shunt surgery group. Mean ± standard error. *P < 0.05 by Wilcoxon rank-sum test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591270&req=5

pone.0139751.g003: Intensity of the bleb wall measured using anterior-segment optical coherence tomography.Representative images in a horizontal scan 1 week after trabeculectomy (A) and Ex-PRESS tube shunt surgery (B). Time course of intensity. Open circles with dotted line, trabeculectomy group; closed circles with solid line, Ex-PRESS tube shunt surgery group. Mean ± standard error. *P < 0.05 by Wilcoxon rank-sum test.
Mentions: Representative blebs are shown in Fig 2A. Similar avascular cystic blebs were observed in the groups. In both groups, the bleb became flat by 3 months after surgery. There were no significant differences in area or vascularity between the groups at any time point after surgery (Fig 2B and 2C). In all eyes after shunt surgery, the device remained attached to the iris at all time points. In the AS-OCT images (Fig 3), only 3 (12.5%) of 24 eyes had a fluid-filled space, and many of the blebs were filled with sponge-like tissue, followed by a vague shadow of the scleral bed. This made it difficult to compare the imaging characteristics (i.e., height and wall thickness of the bleb, height of the fluid-filled cavity, filtration opening in the scleral flap) between the groups. However, the density of the bleb wall was significantly lower 1 week after surgery in the Ex-PRESS group than the trabeculectomy group (P < 0.05). Two days after surgery it was significantly denser than 7 and 14 days postoperatively in the Ex-PRESS group and at 14 days in the trabeculectomy group, suggesting a more rapid decrease in the Ex-PRESS group than in the trabeculectomy group.

Bottom Line: In the Ex-PRESS group, the corresponding values were 1744.0, 1372.0, 932.5, 711.7, 396.1, 487.3, 799.5, 1327.9, 293.6, and 184.0 pg/mL.There were no significant differences in the aqueous MCP-1 level between the groups at any time point.The postoperative aqueous MCP-1 level had bimodal peaks in both groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.

ABSTRACT

Purpose: To evaluate the postoperative changes in blebs and levels of aqueous monocyte chemotactic protein-1 (MCP-1) after trabeculectomy vs. Ex-PRESS tube shunt surgery.

Methods: Rabbits were subjected to trabeculectomy or Ex-PRESS tube shunt surgery and observed for up to 3 months. Intraocular pressure (IOP) was measured using a rebound tonometer. The MCP-1 level was measured by enzyme-linked immunosorbent assay (ELISA). Bleb morphology was evaluated using photos and anterior-segment optical coherence tomography (OCT).

Results: There were no differences in bleb appearance or IOP at any time between the groups. Bleb wall density in the anterior-segment OCT image was significantly lower 1 week after surgery in the Ex-PRESS group than the trabeculectomy group. The MCP-1 level in control eyes was 304.1 ± 45.2 pg/mL. In the trabeculectomy group, the mean aqueous MCP-1 level was 1444.9, 1914.3, 1899.8, 516.4, 398.3, 427.3, 609.5, 1612.7, 386.2, and 167.9 pg/mL at 3, 6, and 12 h, and 1, 2, 5, 7, 14, 30, and 90 days after surgery, respectively. In the Ex-PRESS group, the corresponding values were 1744.0, 1372.0, 932.5, 711.7, 396.1, 487.3, 799.5, 1327.9, 293.6, and 184.0 pg/mL. There were no significant differences in the aqueous MCP-1 level between the groups at any time point.

Conclusion: The postoperative changes were similar in the Ex-PRESS and trabeculectomy groups, except for bleb wall density in the anterior-segment OCT image. The postoperative aqueous MCP-1 level had bimodal peaks in both groups.

No MeSH data available.


Related in: MedlinePlus