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Postoperative Changes in Aqueous Monocyte Chemotactic Protein-1 Levels and Bleb Morphology after Trabeculectomy vs. Ex-PRESS Shunt Surgery.

Shobayashi K, Inoue T, Kawai M, Iwao K, Ohira S, Kojima S, Kuroda U, Nakashima K, Tanihara H - PLoS ONE (2015)

Bottom Line: In the Ex-PRESS group, the corresponding values were 1744.0, 1372.0, 932.5, 711.7, 396.1, 487.3, 799.5, 1327.9, 293.6, and 184.0 pg/mL.There were no significant differences in the aqueous MCP-1 level between the groups at any time point.The postoperative aqueous MCP-1 level had bimodal peaks in both groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.

ABSTRACT

Purpose: To evaluate the postoperative changes in blebs and levels of aqueous monocyte chemotactic protein-1 (MCP-1) after trabeculectomy vs. Ex-PRESS tube shunt surgery.

Methods: Rabbits were subjected to trabeculectomy or Ex-PRESS tube shunt surgery and observed for up to 3 months. Intraocular pressure (IOP) was measured using a rebound tonometer. The MCP-1 level was measured by enzyme-linked immunosorbent assay (ELISA). Bleb morphology was evaluated using photos and anterior-segment optical coherence tomography (OCT).

Results: There were no differences in bleb appearance or IOP at any time between the groups. Bleb wall density in the anterior-segment OCT image was significantly lower 1 week after surgery in the Ex-PRESS group than the trabeculectomy group. The MCP-1 level in control eyes was 304.1 ± 45.2 pg/mL. In the trabeculectomy group, the mean aqueous MCP-1 level was 1444.9, 1914.3, 1899.8, 516.4, 398.3, 427.3, 609.5, 1612.7, 386.2, and 167.9 pg/mL at 3, 6, and 12 h, and 1, 2, 5, 7, 14, 30, and 90 days after surgery, respectively. In the Ex-PRESS group, the corresponding values were 1744.0, 1372.0, 932.5, 711.7, 396.1, 487.3, 799.5, 1327.9, 293.6, and 184.0 pg/mL. There were no significant differences in the aqueous MCP-1 level between the groups at any time point.

Conclusion: The postoperative changes were similar in the Ex-PRESS and trabeculectomy groups, except for bleb wall density in the anterior-segment OCT image. The postoperative aqueous MCP-1 level had bimodal peaks in both groups.

No MeSH data available.


Related in: MedlinePlus

Time course of aqueous MCP-1 (A) and IOP level (B).Open circles with dotted line, trabeculectomy group; closed circles with solid line, Ex-PRESS tube shunt surgery group. Mean ± standard error. *P < 0.05, **P < 0.01, Dunnett’s test compared to non-operated eyes.
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pone.0139751.g001: Time course of aqueous MCP-1 (A) and IOP level (B).Open circles with dotted line, trabeculectomy group; closed circles with solid line, Ex-PRESS tube shunt surgery group. Mean ± standard error. *P < 0.05, **P < 0.01, Dunnett’s test compared to non-operated eyes.

Mentions: Fig 1A shows the time course of the aqueous MCP-1 level after surgery; there were bimodal peaks in both groups. The mean (±SE) MCP-1 level was 304.1 ± 45.2 pg/mL in controls. It was 1444.9 ± 300.0, 1914.3 ± 645.2, 1899.8 ± 515.0, 516.4 ± 158.2, 398.3 ± 73.1, 427.3 ± 65.7, 609.5 ± 124.2, 1612.7 ± 537.2, 386.2 ± 31.0, and 167.9 ± 45.0 pg/mL at 3, 6, 12, 24, and 48 h, and 5, 7, 14, 30, and 90 days after surgery, respectively, in the trabeculectomy group, and 1744.0 ± 376.6, 1372.0 ± 253.1, 932.5 ± 531.7, 711.7 ± 427.2, 396.1 ± 92.7, 487.3 ± 101.3, 799.5 ± 227.0, 1327.9 ± 466.0, 293.6 ± 148.6, and 184.0 ± 25.4 pg/mL in the Ex-PRESS group. There were no significant differences at any time point between the two groups (P = 0.631, 1.000, 0.109, 0.631, 0.749, 0.749, 0.631, 0.873, 0.078, and 0.423, respectively). At 6, 12 h and 14 days after surgery, the MCP-1 level was significantly higher in eyes subjected to trabeculectomy than in controls (P = 0.006, 0.007, and 0.039, respectively). Conversely, the level was significantly higher in eyes that underwent shunt surgery than in control eyes only at 3 h after surgery (P = 0.010).


Postoperative Changes in Aqueous Monocyte Chemotactic Protein-1 Levels and Bleb Morphology after Trabeculectomy vs. Ex-PRESS Shunt Surgery.

Shobayashi K, Inoue T, Kawai M, Iwao K, Ohira S, Kojima S, Kuroda U, Nakashima K, Tanihara H - PLoS ONE (2015)

Time course of aqueous MCP-1 (A) and IOP level (B).Open circles with dotted line, trabeculectomy group; closed circles with solid line, Ex-PRESS tube shunt surgery group. Mean ± standard error. *P < 0.05, **P < 0.01, Dunnett’s test compared to non-operated eyes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591270&req=5

pone.0139751.g001: Time course of aqueous MCP-1 (A) and IOP level (B).Open circles with dotted line, trabeculectomy group; closed circles with solid line, Ex-PRESS tube shunt surgery group. Mean ± standard error. *P < 0.05, **P < 0.01, Dunnett’s test compared to non-operated eyes.
Mentions: Fig 1A shows the time course of the aqueous MCP-1 level after surgery; there were bimodal peaks in both groups. The mean (±SE) MCP-1 level was 304.1 ± 45.2 pg/mL in controls. It was 1444.9 ± 300.0, 1914.3 ± 645.2, 1899.8 ± 515.0, 516.4 ± 158.2, 398.3 ± 73.1, 427.3 ± 65.7, 609.5 ± 124.2, 1612.7 ± 537.2, 386.2 ± 31.0, and 167.9 ± 45.0 pg/mL at 3, 6, 12, 24, and 48 h, and 5, 7, 14, 30, and 90 days after surgery, respectively, in the trabeculectomy group, and 1744.0 ± 376.6, 1372.0 ± 253.1, 932.5 ± 531.7, 711.7 ± 427.2, 396.1 ± 92.7, 487.3 ± 101.3, 799.5 ± 227.0, 1327.9 ± 466.0, 293.6 ± 148.6, and 184.0 ± 25.4 pg/mL in the Ex-PRESS group. There were no significant differences at any time point between the two groups (P = 0.631, 1.000, 0.109, 0.631, 0.749, 0.749, 0.631, 0.873, 0.078, and 0.423, respectively). At 6, 12 h and 14 days after surgery, the MCP-1 level was significantly higher in eyes subjected to trabeculectomy than in controls (P = 0.006, 0.007, and 0.039, respectively). Conversely, the level was significantly higher in eyes that underwent shunt surgery than in control eyes only at 3 h after surgery (P = 0.010).

Bottom Line: In the Ex-PRESS group, the corresponding values were 1744.0, 1372.0, 932.5, 711.7, 396.1, 487.3, 799.5, 1327.9, 293.6, and 184.0 pg/mL.There were no significant differences in the aqueous MCP-1 level between the groups at any time point.The postoperative aqueous MCP-1 level had bimodal peaks in both groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.

ABSTRACT

Purpose: To evaluate the postoperative changes in blebs and levels of aqueous monocyte chemotactic protein-1 (MCP-1) after trabeculectomy vs. Ex-PRESS tube shunt surgery.

Methods: Rabbits were subjected to trabeculectomy or Ex-PRESS tube shunt surgery and observed for up to 3 months. Intraocular pressure (IOP) was measured using a rebound tonometer. The MCP-1 level was measured by enzyme-linked immunosorbent assay (ELISA). Bleb morphology was evaluated using photos and anterior-segment optical coherence tomography (OCT).

Results: There were no differences in bleb appearance or IOP at any time between the groups. Bleb wall density in the anterior-segment OCT image was significantly lower 1 week after surgery in the Ex-PRESS group than the trabeculectomy group. The MCP-1 level in control eyes was 304.1 ± 45.2 pg/mL. In the trabeculectomy group, the mean aqueous MCP-1 level was 1444.9, 1914.3, 1899.8, 516.4, 398.3, 427.3, 609.5, 1612.7, 386.2, and 167.9 pg/mL at 3, 6, and 12 h, and 1, 2, 5, 7, 14, 30, and 90 days after surgery, respectively. In the Ex-PRESS group, the corresponding values were 1744.0, 1372.0, 932.5, 711.7, 396.1, 487.3, 799.5, 1327.9, 293.6, and 184.0 pg/mL. There were no significant differences in the aqueous MCP-1 level between the groups at any time point.

Conclusion: The postoperative changes were similar in the Ex-PRESS and trabeculectomy groups, except for bleb wall density in the anterior-segment OCT image. The postoperative aqueous MCP-1 level had bimodal peaks in both groups.

No MeSH data available.


Related in: MedlinePlus