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The GSTome Reflects the Chemical Environment of White-Rot Fungi.

Deroy A, Saiag F, Kebbi-Benkeder Z, Touahri N, Hecker A, Morel-Rouhier M, Colin F, Dumarcay S, Gérardin P, Gelhaye E - PLoS ONE (2015)

Bottom Line: In other respects, wood durability, among other factors, is due to the presence of extractives that are potential antimicrobial molecules.The results demonstrate that the specificity of these interactions is closely related to the chemical composition of the extracts in accordance with the tree species and their localization inside the wood (sapwood vs heartwood vs knotwood).These data suggest that the fungal GSTome could reflect the chemical environment encountered by these fungi during wood degradation and could be a way to study their adaptation to their way of life.

View Article: PubMed Central - PubMed

Affiliation: Université de Lorraine, Interactions Arbres-Microorganismes, UMR1136, F-54500, Vandoeuvre-lès-Nancy, France; INRA, Interactions Arbres-Microorganismes, UMR1136, F-54280, Champenoux, France.

ABSTRACT
White-rot fungi possess the unique ability to degrade and mineralize all the different components of wood. In other respects, wood durability, among other factors, is due to the presence of extractives that are potential antimicrobial molecules. To cope with these molecules, wood decay fungi have developed a complex detoxification network including glutathione transferases (GST). The interactions between GSTs from two white-rot fungi, Trametes versicolor and Phanerochaete chrysosporium, and an environmental library of wood extracts have been studied. The results demonstrate that the specificity of these interactions is closely related to the chemical composition of the extracts in accordance with the tree species and their localization inside the wood (sapwood vs heartwood vs knotwood). These data suggest that the fungal GSTome could reflect the chemical environment encountered by these fungi during wood degradation and could be a way to study their adaptation to their way of life.

No MeSH data available.


Related in: MedlinePlus

Principal component analysis plot showing the distribution of acetonic extractives of the group B defined in Fig 3.This group B is a chemically similar group of extractives based on major functional categorizations of chemicals found in the extracts. A matrix based on the interactions between the six studied TvGSTO and the five studied PcUre2p and these extractives determined using the fluorescence-based thermal stability assay and the competition experiments was used as input
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pone.0137083.g004: Principal component analysis plot showing the distribution of acetonic extractives of the group B defined in Fig 3.This group B is a chemically similar group of extractives based on major functional categorizations of chemicals found in the extracts. A matrix based on the interactions between the six studied TvGSTO and the five studied PcUre2p and these extractives determined using the fluorescence-based thermal stability assay and the competition experiments was used as input

Mentions: In order to get a more accurate discrimination of the extracts belonging to the previously described group B, another PCA was implemented using FTS and CE data with both TvGSTO and PcUre2p proteins and the group B wood extracts as input. This PCA led to three sub-groups statistically distinct (Fisher analysis on the coordinates, p<0.0001), separating in particular clearly cherry knotwood from cherry heartwood extracts (Fig 4). The presence of sakuranin in cherry heartwood extracts (ANOVA performed on the chemical composition, p<0.0001), and more globally the difference in flavonoids composition could explain this repartition. In accordance the previous analysis (Fig 2), the presence of gallic acid could be related to the observed distribution of the chesnut extracts (p<0.01). To confirm that TvGSTOs and PcUre2ps could indeed interact with gallic acid and flavonoids, we tested the effects of pure compounds, (gallic acid and two flavonoids, epicatechin and quercetin) on the thermostability and the esterase activity of the different tested proteins. The obtained results demonstrate that globally TvGSTOs and PcUre2ps interact with these compounds (modification of the thermostability and inhibition of the esterase activity) (data not shown).


The GSTome Reflects the Chemical Environment of White-Rot Fungi.

Deroy A, Saiag F, Kebbi-Benkeder Z, Touahri N, Hecker A, Morel-Rouhier M, Colin F, Dumarcay S, Gérardin P, Gelhaye E - PLoS ONE (2015)

Principal component analysis plot showing the distribution of acetonic extractives of the group B defined in Fig 3.This group B is a chemically similar group of extractives based on major functional categorizations of chemicals found in the extracts. A matrix based on the interactions between the six studied TvGSTO and the five studied PcUre2p and these extractives determined using the fluorescence-based thermal stability assay and the competition experiments was used as input
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591263&req=5

pone.0137083.g004: Principal component analysis plot showing the distribution of acetonic extractives of the group B defined in Fig 3.This group B is a chemically similar group of extractives based on major functional categorizations of chemicals found in the extracts. A matrix based on the interactions between the six studied TvGSTO and the five studied PcUre2p and these extractives determined using the fluorescence-based thermal stability assay and the competition experiments was used as input
Mentions: In order to get a more accurate discrimination of the extracts belonging to the previously described group B, another PCA was implemented using FTS and CE data with both TvGSTO and PcUre2p proteins and the group B wood extracts as input. This PCA led to three sub-groups statistically distinct (Fisher analysis on the coordinates, p<0.0001), separating in particular clearly cherry knotwood from cherry heartwood extracts (Fig 4). The presence of sakuranin in cherry heartwood extracts (ANOVA performed on the chemical composition, p<0.0001), and more globally the difference in flavonoids composition could explain this repartition. In accordance the previous analysis (Fig 2), the presence of gallic acid could be related to the observed distribution of the chesnut extracts (p<0.01). To confirm that TvGSTOs and PcUre2ps could indeed interact with gallic acid and flavonoids, we tested the effects of pure compounds, (gallic acid and two flavonoids, epicatechin and quercetin) on the thermostability and the esterase activity of the different tested proteins. The obtained results demonstrate that globally TvGSTOs and PcUre2ps interact with these compounds (modification of the thermostability and inhibition of the esterase activity) (data not shown).

Bottom Line: In other respects, wood durability, among other factors, is due to the presence of extractives that are potential antimicrobial molecules.The results demonstrate that the specificity of these interactions is closely related to the chemical composition of the extracts in accordance with the tree species and their localization inside the wood (sapwood vs heartwood vs knotwood).These data suggest that the fungal GSTome could reflect the chemical environment encountered by these fungi during wood degradation and could be a way to study their adaptation to their way of life.

View Article: PubMed Central - PubMed

Affiliation: Université de Lorraine, Interactions Arbres-Microorganismes, UMR1136, F-54500, Vandoeuvre-lès-Nancy, France; INRA, Interactions Arbres-Microorganismes, UMR1136, F-54280, Champenoux, France.

ABSTRACT
White-rot fungi possess the unique ability to degrade and mineralize all the different components of wood. In other respects, wood durability, among other factors, is due to the presence of extractives that are potential antimicrobial molecules. To cope with these molecules, wood decay fungi have developed a complex detoxification network including glutathione transferases (GST). The interactions between GSTs from two white-rot fungi, Trametes versicolor and Phanerochaete chrysosporium, and an environmental library of wood extracts have been studied. The results demonstrate that the specificity of these interactions is closely related to the chemical composition of the extracts in accordance with the tree species and their localization inside the wood (sapwood vs heartwood vs knotwood). These data suggest that the fungal GSTome could reflect the chemical environment encountered by these fungi during wood degradation and could be a way to study their adaptation to their way of life.

No MeSH data available.


Related in: MedlinePlus