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The GSTome Reflects the Chemical Environment of White-Rot Fungi.

Deroy A, Saiag F, Kebbi-Benkeder Z, Touahri N, Hecker A, Morel-Rouhier M, Colin F, Dumarcay S, Gérardin P, Gelhaye E - PLoS ONE (2015)

Bottom Line: In other respects, wood durability, among other factors, is due to the presence of extractives that are potential antimicrobial molecules.The results demonstrate that the specificity of these interactions is closely related to the chemical composition of the extracts in accordance with the tree species and their localization inside the wood (sapwood vs heartwood vs knotwood).These data suggest that the fungal GSTome could reflect the chemical environment encountered by these fungi during wood degradation and could be a way to study their adaptation to their way of life.

View Article: PubMed Central - PubMed

Affiliation: Université de Lorraine, Interactions Arbres-Microorganismes, UMR1136, F-54500, Vandoeuvre-lès-Nancy, France; INRA, Interactions Arbres-Microorganismes, UMR1136, F-54280, Champenoux, France.

ABSTRACT
White-rot fungi possess the unique ability to degrade and mineralize all the different components of wood. In other respects, wood durability, among other factors, is due to the presence of extractives that are potential antimicrobial molecules. To cope with these molecules, wood decay fungi have developed a complex detoxification network including glutathione transferases (GST). The interactions between GSTs from two white-rot fungi, Trametes versicolor and Phanerochaete chrysosporium, and an environmental library of wood extracts have been studied. The results demonstrate that the specificity of these interactions is closely related to the chemical composition of the extracts in accordance with the tree species and their localization inside the wood (sapwood vs heartwood vs knotwood). These data suggest that the fungal GSTome could reflect the chemical environment encountered by these fungi during wood degradation and could be a way to study their adaptation to their way of life.

No MeSH data available.


Related in: MedlinePlus

Principal component analysis plot showing the distribution of six GST Omega (GSTO) from Trametes versicolor and five Ure2p from Phanerochaete chrysosporium.A matrix based on the interactions, measured using the fluorescence-based thermal stability assay (squares) and the competition experiments (circle), between 60 wood extracts and these tested GSTs was used as input.
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pone.0137083.g002: Principal component analysis plot showing the distribution of six GST Omega (GSTO) from Trametes versicolor and five Ure2p from Phanerochaete chrysosporium.A matrix based on the interactions, measured using the fluorescence-based thermal stability assay (squares) and the competition experiments (circle), between 60 wood extracts and these tested GSTs was used as input.

Mentions: A principal components analysis (PCA) was implemented using the normalized obtained data from FTS and CE experiments as input (The used matrix is shown in S2 Table). The tested proteins were found widespread in the obtained biplot (PC1 and PC2 accounted of 56.59% of the total variance) without a clear clustering (Fig 2). Both approaches (FTS and CE) group some proteins as TvGSTO2S, TvGSTO4S or PcUre2pA8. In contrast, both methods gave different results for others such as PcUre2pB1 and TvGSTO6S for instance, suggesting that in this case ligandin (L site) and catalytic domains (G and/or H sites) are clearly different. These data confirm that FTS and CE approaches are complementary and their combination could be a way to investigate the relationship between the diversity of fungal GSTs and the variability of wood extracts.


The GSTome Reflects the Chemical Environment of White-Rot Fungi.

Deroy A, Saiag F, Kebbi-Benkeder Z, Touahri N, Hecker A, Morel-Rouhier M, Colin F, Dumarcay S, Gérardin P, Gelhaye E - PLoS ONE (2015)

Principal component analysis plot showing the distribution of six GST Omega (GSTO) from Trametes versicolor and five Ure2p from Phanerochaete chrysosporium.A matrix based on the interactions, measured using the fluorescence-based thermal stability assay (squares) and the competition experiments (circle), between 60 wood extracts and these tested GSTs was used as input.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591263&req=5

pone.0137083.g002: Principal component analysis plot showing the distribution of six GST Omega (GSTO) from Trametes versicolor and five Ure2p from Phanerochaete chrysosporium.A matrix based on the interactions, measured using the fluorescence-based thermal stability assay (squares) and the competition experiments (circle), between 60 wood extracts and these tested GSTs was used as input.
Mentions: A principal components analysis (PCA) was implemented using the normalized obtained data from FTS and CE experiments as input (The used matrix is shown in S2 Table). The tested proteins were found widespread in the obtained biplot (PC1 and PC2 accounted of 56.59% of the total variance) without a clear clustering (Fig 2). Both approaches (FTS and CE) group some proteins as TvGSTO2S, TvGSTO4S or PcUre2pA8. In contrast, both methods gave different results for others such as PcUre2pB1 and TvGSTO6S for instance, suggesting that in this case ligandin (L site) and catalytic domains (G and/or H sites) are clearly different. These data confirm that FTS and CE approaches are complementary and their combination could be a way to investigate the relationship between the diversity of fungal GSTs and the variability of wood extracts.

Bottom Line: In other respects, wood durability, among other factors, is due to the presence of extractives that are potential antimicrobial molecules.The results demonstrate that the specificity of these interactions is closely related to the chemical composition of the extracts in accordance with the tree species and their localization inside the wood (sapwood vs heartwood vs knotwood).These data suggest that the fungal GSTome could reflect the chemical environment encountered by these fungi during wood degradation and could be a way to study their adaptation to their way of life.

View Article: PubMed Central - PubMed

Affiliation: Université de Lorraine, Interactions Arbres-Microorganismes, UMR1136, F-54500, Vandoeuvre-lès-Nancy, France; INRA, Interactions Arbres-Microorganismes, UMR1136, F-54280, Champenoux, France.

ABSTRACT
White-rot fungi possess the unique ability to degrade and mineralize all the different components of wood. In other respects, wood durability, among other factors, is due to the presence of extractives that are potential antimicrobial molecules. To cope with these molecules, wood decay fungi have developed a complex detoxification network including glutathione transferases (GST). The interactions between GSTs from two white-rot fungi, Trametes versicolor and Phanerochaete chrysosporium, and an environmental library of wood extracts have been studied. The results demonstrate that the specificity of these interactions is closely related to the chemical composition of the extracts in accordance with the tree species and their localization inside the wood (sapwood vs heartwood vs knotwood). These data suggest that the fungal GSTome could reflect the chemical environment encountered by these fungi during wood degradation and could be a way to study their adaptation to their way of life.

No MeSH data available.


Related in: MedlinePlus