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The GSTome Reflects the Chemical Environment of White-Rot Fungi.

Deroy A, Saiag F, Kebbi-Benkeder Z, Touahri N, Hecker A, Morel-Rouhier M, Colin F, Dumarcay S, Gérardin P, Gelhaye E - PLoS ONE (2015)

Bottom Line: In other respects, wood durability, among other factors, is due to the presence of extractives that are potential antimicrobial molecules.The results demonstrate that the specificity of these interactions is closely related to the chemical composition of the extracts in accordance with the tree species and their localization inside the wood (sapwood vs heartwood vs knotwood).These data suggest that the fungal GSTome could reflect the chemical environment encountered by these fungi during wood degradation and could be a way to study their adaptation to their way of life.

View Article: PubMed Central - PubMed

Affiliation: Université de Lorraine, Interactions Arbres-Microorganismes, UMR1136, F-54500, Vandoeuvre-lès-Nancy, France; INRA, Interactions Arbres-Microorganismes, UMR1136, F-54280, Champenoux, France.

ABSTRACT
White-rot fungi possess the unique ability to degrade and mineralize all the different components of wood. In other respects, wood durability, among other factors, is due to the presence of extractives that are potential antimicrobial molecules. To cope with these molecules, wood decay fungi have developed a complex detoxification network including glutathione transferases (GST). The interactions between GSTs from two white-rot fungi, Trametes versicolor and Phanerochaete chrysosporium, and an environmental library of wood extracts have been studied. The results demonstrate that the specificity of these interactions is closely related to the chemical composition of the extracts in accordance with the tree species and their localization inside the wood (sapwood vs heartwood vs knotwood). These data suggest that the fungal GSTome could reflect the chemical environment encountered by these fungi during wood degradation and could be a way to study their adaptation to their way of life.

No MeSH data available.


Related in: MedlinePlus

Effects of larch acetonic extracts on the catalytic activity (A) and thermal stability (B) of TvGSTO-1S.The catalytic activity was monitored following the cleavage of CMFDA by fluorescence as described in materials and methods section. DMSO or the tested extracts have been added after 10 min. For the thermal stability, the first derivatives of the raw data are shown allowing to determinate the Tm. diamond: DMSO (control); square: larch sapwood; triangle: larch heartwood
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pone.0137083.g001: Effects of larch acetonic extracts on the catalytic activity (A) and thermal stability (B) of TvGSTO-1S.The catalytic activity was monitored following the cleavage of CMFDA by fluorescence as described in materials and methods section. DMSO or the tested extracts have been added after 10 min. For the thermal stability, the first derivatives of the raw data are shown allowing to determinate the Tm. diamond: DMSO (control); square: larch sapwood; triangle: larch heartwood

Mentions: FTS and competition experiments using CMFDA were then combined to study the interactions between the studied GSTs and an environmental library composed of 60 different wood extracts. This library contained acetone and dichloromethane extracts of sapwood, heartwood and knotwood from different hardwood and softwood species (S4 Table). A shift on the protein melting temperature (Tm) revealed an interaction the tested product and the protein, whereas an inhibition of the CMFDA transformation revealed an interaction between the tested product and either the G site, or the H site or both. As an example, the effects of acetonic extracts from larch heartwood and sapwood on TvGSTO1-S catalytic and ligandin activities are shown in Fig 1. The heartwood extract induced both an inhibition of the catalytic activity (Fig 1A) and a shift of the measured Tm (Fig 1B), whereas no effects were observed in presence of sapwood extracts in comparison with the control (only DMSO).


The GSTome Reflects the Chemical Environment of White-Rot Fungi.

Deroy A, Saiag F, Kebbi-Benkeder Z, Touahri N, Hecker A, Morel-Rouhier M, Colin F, Dumarcay S, Gérardin P, Gelhaye E - PLoS ONE (2015)

Effects of larch acetonic extracts on the catalytic activity (A) and thermal stability (B) of TvGSTO-1S.The catalytic activity was monitored following the cleavage of CMFDA by fluorescence as described in materials and methods section. DMSO or the tested extracts have been added after 10 min. For the thermal stability, the first derivatives of the raw data are shown allowing to determinate the Tm. diamond: DMSO (control); square: larch sapwood; triangle: larch heartwood
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591263&req=5

pone.0137083.g001: Effects of larch acetonic extracts on the catalytic activity (A) and thermal stability (B) of TvGSTO-1S.The catalytic activity was monitored following the cleavage of CMFDA by fluorescence as described in materials and methods section. DMSO or the tested extracts have been added after 10 min. For the thermal stability, the first derivatives of the raw data are shown allowing to determinate the Tm. diamond: DMSO (control); square: larch sapwood; triangle: larch heartwood
Mentions: FTS and competition experiments using CMFDA were then combined to study the interactions between the studied GSTs and an environmental library composed of 60 different wood extracts. This library contained acetone and dichloromethane extracts of sapwood, heartwood and knotwood from different hardwood and softwood species (S4 Table). A shift on the protein melting temperature (Tm) revealed an interaction the tested product and the protein, whereas an inhibition of the CMFDA transformation revealed an interaction between the tested product and either the G site, or the H site or both. As an example, the effects of acetonic extracts from larch heartwood and sapwood on TvGSTO1-S catalytic and ligandin activities are shown in Fig 1. The heartwood extract induced both an inhibition of the catalytic activity (Fig 1A) and a shift of the measured Tm (Fig 1B), whereas no effects were observed in presence of sapwood extracts in comparison with the control (only DMSO).

Bottom Line: In other respects, wood durability, among other factors, is due to the presence of extractives that are potential antimicrobial molecules.The results demonstrate that the specificity of these interactions is closely related to the chemical composition of the extracts in accordance with the tree species and their localization inside the wood (sapwood vs heartwood vs knotwood).These data suggest that the fungal GSTome could reflect the chemical environment encountered by these fungi during wood degradation and could be a way to study their adaptation to their way of life.

View Article: PubMed Central - PubMed

Affiliation: Université de Lorraine, Interactions Arbres-Microorganismes, UMR1136, F-54500, Vandoeuvre-lès-Nancy, France; INRA, Interactions Arbres-Microorganismes, UMR1136, F-54280, Champenoux, France.

ABSTRACT
White-rot fungi possess the unique ability to degrade and mineralize all the different components of wood. In other respects, wood durability, among other factors, is due to the presence of extractives that are potential antimicrobial molecules. To cope with these molecules, wood decay fungi have developed a complex detoxification network including glutathione transferases (GST). The interactions between GSTs from two white-rot fungi, Trametes versicolor and Phanerochaete chrysosporium, and an environmental library of wood extracts have been studied. The results demonstrate that the specificity of these interactions is closely related to the chemical composition of the extracts in accordance with the tree species and their localization inside the wood (sapwood vs heartwood vs knotwood). These data suggest that the fungal GSTome could reflect the chemical environment encountered by these fungi during wood degradation and could be a way to study their adaptation to their way of life.

No MeSH data available.


Related in: MedlinePlus