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Pathological features of proteinuric nephropathy resembling Alport syndrome in a young Pyrenean Mountain dog.

Sugahara G, Naito I, Miyagawa Y, Komiyama T, Takemura N, Kobayashi R, Mineshige T, Kamiie J, Shirota K - J. Vet. Med. Sci. (2015)

Bottom Line: Ultrastructural examination revealed multilaminar splitting and fragmentation of the glomerular basement membrane (GBM) and diffuse podocyte foot process effacement.Immunofluorescent staining for α(IV) chains revealed presence of α5(IV) and complete absence of α3(IV) and α4(IV) chains in the GBM.Immunohistochemistry also revealed decreased and altered expression of nephrin and podocin in the glomeruli compared with normal canine glomeruli.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.

ABSTRACT
The renal biopsy tissue from a 9-month-old, male Pyrenean Mountain dog with renal disorder and severe proteinuria was examined. Ultrastructural examination revealed multilaminar splitting and fragmentation of the glomerular basement membrane (GBM) and diffuse podocyte foot process effacement. Immunofluorescent staining for α(IV) chains revealed presence of α5(IV) and complete absence of α3(IV) and α4(IV) chains in the GBM. Immunohistochemistry also revealed decreased and altered expression of nephrin and podocin in the glomeruli compared with normal canine glomeruli. These results suggested that the glomerular disease of the present case might be consistent with canine hereditary nephropathy resembling human Alport syndrome caused by genetic defect of type IV collagen, and indicated possible contribution of podocyte injury to severe proteinuria in this case.

No MeSH data available.


Related in: MedlinePlus

Immunofluorescence staining for nephrin (A) and podocin (B) in the glomeruli of thecase and a normal dog (respective insets). Expression of both molecules in the case wasdecreased, and the staining patterns were shifted from the sharp linear pattern observedalong the glomerular capillary walls of normal glomeruli to a coarse granular pattern.IF. Bar, 3 µm.
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fig_004: Immunofluorescence staining for nephrin (A) and podocin (B) in the glomeruli of thecase and a normal dog (respective insets). Expression of both molecules in the case wasdecreased, and the staining patterns were shifted from the sharp linear pattern observedalong the glomerular capillary walls of normal glomeruli to a coarse granular pattern.IF. Bar, 3 µm.

Mentions: Expression of nephrin and podocin was in a sharp linear pattern along the GBM in the normalglomerulus. On the other hand, the nephrin staining pattern was shifted to a cytoplasmic andgranular pattern in the case (Fig. 4AFig. 4.


Pathological features of proteinuric nephropathy resembling Alport syndrome in a young Pyrenean Mountain dog.

Sugahara G, Naito I, Miyagawa Y, Komiyama T, Takemura N, Kobayashi R, Mineshige T, Kamiie J, Shirota K - J. Vet. Med. Sci. (2015)

Immunofluorescence staining for nephrin (A) and podocin (B) in the glomeruli of thecase and a normal dog (respective insets). Expression of both molecules in the case wasdecreased, and the staining patterns were shifted from the sharp linear pattern observedalong the glomerular capillary walls of normal glomeruli to a coarse granular pattern.IF. Bar, 3 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591163&req=5

fig_004: Immunofluorescence staining for nephrin (A) and podocin (B) in the glomeruli of thecase and a normal dog (respective insets). Expression of both molecules in the case wasdecreased, and the staining patterns were shifted from the sharp linear pattern observedalong the glomerular capillary walls of normal glomeruli to a coarse granular pattern.IF. Bar, 3 µm.
Mentions: Expression of nephrin and podocin was in a sharp linear pattern along the GBM in the normalglomerulus. On the other hand, the nephrin staining pattern was shifted to a cytoplasmic andgranular pattern in the case (Fig. 4AFig. 4.

Bottom Line: Ultrastructural examination revealed multilaminar splitting and fragmentation of the glomerular basement membrane (GBM) and diffuse podocyte foot process effacement.Immunofluorescent staining for α(IV) chains revealed presence of α5(IV) and complete absence of α3(IV) and α4(IV) chains in the GBM.Immunohistochemistry also revealed decreased and altered expression of nephrin and podocin in the glomeruli compared with normal canine glomeruli.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.

ABSTRACT
The renal biopsy tissue from a 9-month-old, male Pyrenean Mountain dog with renal disorder and severe proteinuria was examined. Ultrastructural examination revealed multilaminar splitting and fragmentation of the glomerular basement membrane (GBM) and diffuse podocyte foot process effacement. Immunofluorescent staining for α(IV) chains revealed presence of α5(IV) and complete absence of α3(IV) and α4(IV) chains in the GBM. Immunohistochemistry also revealed decreased and altered expression of nephrin and podocin in the glomeruli compared with normal canine glomeruli. These results suggested that the glomerular disease of the present case might be consistent with canine hereditary nephropathy resembling human Alport syndrome caused by genetic defect of type IV collagen, and indicated possible contribution of podocyte injury to severe proteinuria in this case.

No MeSH data available.


Related in: MedlinePlus