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Pathological features of proteinuric nephropathy resembling Alport syndrome in a young Pyrenean Mountain dog.

Sugahara G, Naito I, Miyagawa Y, Komiyama T, Takemura N, Kobayashi R, Mineshige T, Kamiie J, Shirota K - J. Vet. Med. Sci. (2015)

Bottom Line: Ultrastructural examination revealed multilaminar splitting and fragmentation of the glomerular basement membrane (GBM) and diffuse podocyte foot process effacement.Immunofluorescent staining for α(IV) chains revealed presence of α5(IV) and complete absence of α3(IV) and α4(IV) chains in the GBM.Immunohistochemistry also revealed decreased and altered expression of nephrin and podocin in the glomeruli compared with normal canine glomeruli.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.

ABSTRACT
The renal biopsy tissue from a 9-month-old, male Pyrenean Mountain dog with renal disorder and severe proteinuria was examined. Ultrastructural examination revealed multilaminar splitting and fragmentation of the glomerular basement membrane (GBM) and diffuse podocyte foot process effacement. Immunofluorescent staining for α(IV) chains revealed presence of α5(IV) and complete absence of α3(IV) and α4(IV) chains in the GBM. Immunohistochemistry also revealed decreased and altered expression of nephrin and podocin in the glomeruli compared with normal canine glomeruli. These results suggested that the glomerular disease of the present case might be consistent with canine hereditary nephropathy resembling human Alport syndrome caused by genetic defect of type IV collagen, and indicated possible contribution of podocyte injury to severe proteinuria in this case.

No MeSH data available.


Related in: MedlinePlus

Immunofluorescence for several α(IV) chains in the glomeruli of the case and a normaldog (respective insets). α1(IV) and α2(IV) chains (A and B, respectively) were increasedin the GBM; α3(IV) and α4(IV) chains (C and D, respectively) were completely absent inthe GBM; α5(IV) and α6(IV) chains (E and F, respectively) were observed in both the GBMand Bowman’s capsule BM. IF. ×400. Inset: expression pattern of respective α(IV) chainsin the normal glomeruli.
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fig_003: Immunofluorescence for several α(IV) chains in the glomeruli of the case and a normaldog (respective insets). α1(IV) and α2(IV) chains (A and B, respectively) were increasedin the GBM; α3(IV) and α4(IV) chains (C and D, respectively) were completely absent inthe GBM; α5(IV) and α6(IV) chains (E and F, respectively) were observed in both the GBMand Bowman’s capsule BM. IF. ×400. Inset: expression pattern of respective α(IV) chainsin the normal glomeruli.

Mentions: By IF, the pattern of labeling for each α(IV) chain in the case was different from that inthe normal dog. The α1(IV) and α2(IV) chains were distributed in all basement membranes (BM)and mesangial regions in both animals; however, an increased intensity of these chains wasobserved in the case (Fig. 3A and 3BFig. 3.


Pathological features of proteinuric nephropathy resembling Alport syndrome in a young Pyrenean Mountain dog.

Sugahara G, Naito I, Miyagawa Y, Komiyama T, Takemura N, Kobayashi R, Mineshige T, Kamiie J, Shirota K - J. Vet. Med. Sci. (2015)

Immunofluorescence for several α(IV) chains in the glomeruli of the case and a normaldog (respective insets). α1(IV) and α2(IV) chains (A and B, respectively) were increasedin the GBM; α3(IV) and α4(IV) chains (C and D, respectively) were completely absent inthe GBM; α5(IV) and α6(IV) chains (E and F, respectively) were observed in both the GBMand Bowman’s capsule BM. IF. ×400. Inset: expression pattern of respective α(IV) chainsin the normal glomeruli.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591163&req=5

fig_003: Immunofluorescence for several α(IV) chains in the glomeruli of the case and a normaldog (respective insets). α1(IV) and α2(IV) chains (A and B, respectively) were increasedin the GBM; α3(IV) and α4(IV) chains (C and D, respectively) were completely absent inthe GBM; α5(IV) and α6(IV) chains (E and F, respectively) were observed in both the GBMand Bowman’s capsule BM. IF. ×400. Inset: expression pattern of respective α(IV) chainsin the normal glomeruli.
Mentions: By IF, the pattern of labeling for each α(IV) chain in the case was different from that inthe normal dog. The α1(IV) and α2(IV) chains were distributed in all basement membranes (BM)and mesangial regions in both animals; however, an increased intensity of these chains wasobserved in the case (Fig. 3A and 3BFig. 3.

Bottom Line: Ultrastructural examination revealed multilaminar splitting and fragmentation of the glomerular basement membrane (GBM) and diffuse podocyte foot process effacement.Immunofluorescent staining for α(IV) chains revealed presence of α5(IV) and complete absence of α3(IV) and α4(IV) chains in the GBM.Immunohistochemistry also revealed decreased and altered expression of nephrin and podocin in the glomeruli compared with normal canine glomeruli.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252-5201, Japan.

ABSTRACT
The renal biopsy tissue from a 9-month-old, male Pyrenean Mountain dog with renal disorder and severe proteinuria was examined. Ultrastructural examination revealed multilaminar splitting and fragmentation of the glomerular basement membrane (GBM) and diffuse podocyte foot process effacement. Immunofluorescent staining for α(IV) chains revealed presence of α5(IV) and complete absence of α3(IV) and α4(IV) chains in the GBM. Immunohistochemistry also revealed decreased and altered expression of nephrin and podocin in the glomeruli compared with normal canine glomeruli. These results suggested that the glomerular disease of the present case might be consistent with canine hereditary nephropathy resembling human Alport syndrome caused by genetic defect of type IV collagen, and indicated possible contribution of podocyte injury to severe proteinuria in this case.

No MeSH data available.


Related in: MedlinePlus