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Phylogenetic analysis of Newcastle disease viruses isolated from wild birds in the Poyang Lake region of China.

Fan S, Wang T, Gao X, Ying Y, Li X, Li Y, Li Y, Ma J, Sun H, Chu D, Xu Y, Yang S, Li Q, Gao Y, Xia X - J. Vet. Med. Sci. (2015)

Bottom Line: The strains were highly identical and were more species specific (chicken and waterfowl) than site specific (Nanchang and Duchang regions).The close phylogenetic proximity of these isolates indicates that viral transmission may happen between poultry and wild birds.Our study demonstrates that lentogenic class I NDVs exist in clinically healthy wild waterfowl and poultry within the Poyang Lake region.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, PR China.

ABSTRACT
Newcastle disease virus (NDV) causes a highly contagious viral disease in poultry and wild birds, and it can cause significant economic loss worldwide. Eight viral strains were isolated by inoculating embryonated chicken eggs from the Poyang Lake region of China with swab samples. All eight of the NDV isolates were identified as class I genotype 3 strains, but they diverged notablely from class II viruses. Further analysis revealed that all eight NDV isolates were lentogenic strains containing the (112)ERQER↓L(117) motif at the F protein cleavage site. The strains were highly identical and were more species specific (chicken and waterfowl) than site specific (Nanchang and Duchang regions). The close phylogenetic proximity of these isolates indicates that viral transmission may happen between poultry and wild birds. Our study demonstrates that lentogenic class I NDVs exist in clinically healthy wild waterfowl and poultry within the Poyang Lake region. Active surveillance of these viruses to determine their evolution and origin is one of the most realistic strategies for preventing and controlling NDV outbreaks.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analysis of the nucleotide sequences of class I genotype 3 HN genesequences (nt 1–2,001). The sequences in our study are indicated by circles (Nanchangstrains) and triangles (Duchang strains). Bootstrap values (based on 1,000 replicates)for each node are provided if >75% of the values were available.
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fig_003: Phylogenetic analysis of the nucleotide sequences of class I genotype 3 HN genesequences (nt 1–2,001). The sequences in our study are indicated by circles (Nanchangstrains) and triangles (Duchang strains). Bootstrap values (based on 1,000 replicates)for each node are provided if >75% of the values were available.

Mentions: Phylogenetic analysis of the nucleotide sequences of the NDV F gene (nt 1–1,662).Thesequences in our study are indicated by circles (Nanchang strains) and triangles(Duchang strains). Bootstrap values (based on 1,000 replicates) for each node areprovided if >75% of the values were available.


Phylogenetic analysis of Newcastle disease viruses isolated from wild birds in the Poyang Lake region of China.

Fan S, Wang T, Gao X, Ying Y, Li X, Li Y, Li Y, Ma J, Sun H, Chu D, Xu Y, Yang S, Li Q, Gao Y, Xia X - J. Vet. Med. Sci. (2015)

Phylogenetic analysis of the nucleotide sequences of class I genotype 3 HN genesequences (nt 1–2,001). The sequences in our study are indicated by circles (Nanchangstrains) and triangles (Duchang strains). Bootstrap values (based on 1,000 replicates)for each node are provided if >75% of the values were available.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591157&req=5

fig_003: Phylogenetic analysis of the nucleotide sequences of class I genotype 3 HN genesequences (nt 1–2,001). The sequences in our study are indicated by circles (Nanchangstrains) and triangles (Duchang strains). Bootstrap values (based on 1,000 replicates)for each node are provided if >75% of the values were available.
Mentions: Phylogenetic analysis of the nucleotide sequences of the NDV F gene (nt 1–1,662).Thesequences in our study are indicated by circles (Nanchang strains) and triangles(Duchang strains). Bootstrap values (based on 1,000 replicates) for each node areprovided if >75% of the values were available.

Bottom Line: The strains were highly identical and were more species specific (chicken and waterfowl) than site specific (Nanchang and Duchang regions).The close phylogenetic proximity of these isolates indicates that viral transmission may happen between poultry and wild birds.Our study demonstrates that lentogenic class I NDVs exist in clinically healthy wild waterfowl and poultry within the Poyang Lake region.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, PR China.

ABSTRACT
Newcastle disease virus (NDV) causes a highly contagious viral disease in poultry and wild birds, and it can cause significant economic loss worldwide. Eight viral strains were isolated by inoculating embryonated chicken eggs from the Poyang Lake region of China with swab samples. All eight of the NDV isolates were identified as class I genotype 3 strains, but they diverged notablely from class II viruses. Further analysis revealed that all eight NDV isolates were lentogenic strains containing the (112)ERQER↓L(117) motif at the F protein cleavage site. The strains were highly identical and were more species specific (chicken and waterfowl) than site specific (Nanchang and Duchang regions). The close phylogenetic proximity of these isolates indicates that viral transmission may happen between poultry and wild birds. Our study demonstrates that lentogenic class I NDVs exist in clinically healthy wild waterfowl and poultry within the Poyang Lake region. Active surveillance of these viruses to determine their evolution and origin is one of the most realistic strategies for preventing and controlling NDV outbreaks.

No MeSH data available.


Related in: MedlinePlus