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Phylogenetic analysis of Newcastle disease viruses isolated from wild birds in the Poyang Lake region of China.

Fan S, Wang T, Gao X, Ying Y, Li X, Li Y, Li Y, Ma J, Sun H, Chu D, Xu Y, Yang S, Li Q, Gao Y, Xia X - J. Vet. Med. Sci. (2015)

Bottom Line: The strains were highly identical and were more species specific (chicken and waterfowl) than site specific (Nanchang and Duchang regions).The close phylogenetic proximity of these isolates indicates that viral transmission may happen between poultry and wild birds.Our study demonstrates that lentogenic class I NDVs exist in clinically healthy wild waterfowl and poultry within the Poyang Lake region.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, PR China.

ABSTRACT
Newcastle disease virus (NDV) causes a highly contagious viral disease in poultry and wild birds, and it can cause significant economic loss worldwide. Eight viral strains were isolated by inoculating embryonated chicken eggs from the Poyang Lake region of China with swab samples. All eight of the NDV isolates were identified as class I genotype 3 strains, but they diverged notablely from class II viruses. Further analysis revealed that all eight NDV isolates were lentogenic strains containing the (112)ERQER↓L(117) motif at the F protein cleavage site. The strains were highly identical and were more species specific (chicken and waterfowl) than site specific (Nanchang and Duchang regions). The close phylogenetic proximity of these isolates indicates that viral transmission may happen between poultry and wild birds. Our study demonstrates that lentogenic class I NDVs exist in clinically healthy wild waterfowl and poultry within the Poyang Lake region. Active surveillance of these viruses to determine their evolution and origin is one of the most realistic strategies for preventing and controlling NDV outbreaks.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analysis of the nucleotide sequences of the NDV F gene (nt 1–1,662).Thesequences in our study are indicated by circles (Nanchang strains) and triangles(Duchang strains). Bootstrap values (based on 1,000 replicates) for each node areprovided if >75% of the values were available.
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fig_002: Phylogenetic analysis of the nucleotide sequences of the NDV F gene (nt 1–1,662).Thesequences in our study are indicated by circles (Nanchang strains) and triangles(Duchang strains). Bootstrap values (based on 1,000 replicates) for each node areprovided if >75% of the values were available.

Mentions: To estimate the risk of the viruses pose to poultry populations, it is essential to evaluatethe evolution of these viruses. Prior to phylogenetic analysis, ClustalX 2.0 [19] and Lasergene V 7.1 software packages were used forsequences analysis. MEGA 5.0 software was used for phylogenetic analysis using aneighbor-joining method with 1,000 bootstrap replicates [28]. The strains of F gene are shown in Table1. Phylogenetic analyses using the 1,662-bp region of the F gene and the 2001-bpregion of the HN gene sequence indicate that all eight NDV strains form a distinct clusterwithin the genotype 3 viruses and are most closely related to Chinese poultry isolates. Aphylogenetic analysis of F gene revealed that the isolated strains were highly identical andwere more species specific (chicken and waterfowl) than site specific (Nanchang and Duchangregions). Two branches radiate out from class I genotype 3 (Fig. 2Fig. 2.


Phylogenetic analysis of Newcastle disease viruses isolated from wild birds in the Poyang Lake region of China.

Fan S, Wang T, Gao X, Ying Y, Li X, Li Y, Li Y, Ma J, Sun H, Chu D, Xu Y, Yang S, Li Q, Gao Y, Xia X - J. Vet. Med. Sci. (2015)

Phylogenetic analysis of the nucleotide sequences of the NDV F gene (nt 1–1,662).Thesequences in our study are indicated by circles (Nanchang strains) and triangles(Duchang strains). Bootstrap values (based on 1,000 replicates) for each node areprovided if >75% of the values were available.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591157&req=5

fig_002: Phylogenetic analysis of the nucleotide sequences of the NDV F gene (nt 1–1,662).Thesequences in our study are indicated by circles (Nanchang strains) and triangles(Duchang strains). Bootstrap values (based on 1,000 replicates) for each node areprovided if >75% of the values were available.
Mentions: To estimate the risk of the viruses pose to poultry populations, it is essential to evaluatethe evolution of these viruses. Prior to phylogenetic analysis, ClustalX 2.0 [19] and Lasergene V 7.1 software packages were used forsequences analysis. MEGA 5.0 software was used for phylogenetic analysis using aneighbor-joining method with 1,000 bootstrap replicates [28]. The strains of F gene are shown in Table1. Phylogenetic analyses using the 1,662-bp region of the F gene and the 2001-bpregion of the HN gene sequence indicate that all eight NDV strains form a distinct clusterwithin the genotype 3 viruses and are most closely related to Chinese poultry isolates. Aphylogenetic analysis of F gene revealed that the isolated strains were highly identical andwere more species specific (chicken and waterfowl) than site specific (Nanchang and Duchangregions). Two branches radiate out from class I genotype 3 (Fig. 2Fig. 2.

Bottom Line: The strains were highly identical and were more species specific (chicken and waterfowl) than site specific (Nanchang and Duchang regions).The close phylogenetic proximity of these isolates indicates that viral transmission may happen between poultry and wild birds.Our study demonstrates that lentogenic class I NDVs exist in clinically healthy wild waterfowl and poultry within the Poyang Lake region.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, PR China.

ABSTRACT
Newcastle disease virus (NDV) causes a highly contagious viral disease in poultry and wild birds, and it can cause significant economic loss worldwide. Eight viral strains were isolated by inoculating embryonated chicken eggs from the Poyang Lake region of China with swab samples. All eight of the NDV isolates were identified as class I genotype 3 strains, but they diverged notablely from class II viruses. Further analysis revealed that all eight NDV isolates were lentogenic strains containing the (112)ERQER↓L(117) motif at the F protein cleavage site. The strains were highly identical and were more species specific (chicken and waterfowl) than site specific (Nanchang and Duchang regions). The close phylogenetic proximity of these isolates indicates that viral transmission may happen between poultry and wild birds. Our study demonstrates that lentogenic class I NDVs exist in clinically healthy wild waterfowl and poultry within the Poyang Lake region. Active surveillance of these viruses to determine their evolution and origin is one of the most realistic strategies for preventing and controlling NDV outbreaks.

No MeSH data available.


Related in: MedlinePlus