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Improvement of bacterial clearance and relief of clinical signs of Salmonella enterica serovar Typhimurium infection in pigs through upregulation of Th 1-specific responses by administration of a combination of two silicate minerals, biotite and bentonite.

Lee JA, Jung BG, Kim TH, Kim YM, Koh HB, Lee BJ - J. Vet. Med. Sci. (2015)

Bottom Line: Typhimurium.Typhimurium and the number of viable bacteria in feces and tissues were significantly decreased in both BBM groups, especially in the 2% BBM group.Therefore, the BBM could be a good candidate as an alternative antibiotic that improves Th 1-specific immune responses and the bacterial clearance effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea.

ABSTRACT
Biotite and bentonite are phyllosilicate minerals that were originally used in industrial applications. Several beneficial activities of them have recently been reported, especially regulation of the immune system and antimicrobial effects. Therefore, we investigated the immune-enhancing and bacterial clearance effects of a biotite and bentonite mixture (BBM) on experimental infection of Salmonella enterica serovar Typhimurium (S. Typhimurium) to determine whether the BBM could be used as an alternative antibiotic. We administered 1% or 2% BBM as a feed supplement. We then evaluated the bacterial clearance effects of the BBM against S. Typhimurium. We also evaluated the immune-enhancing effect of the BBM through several immunological experiments that included examination of the lysozyme activity, CD4(+)/CD8(+) T lymphocyte ratio and the T-helper type 1 (Th 1) cytokine profile. The clinical signs of S. Typhimurium and the number of viable bacteria in feces and tissues were significantly decreased in both BBM groups, especially in the 2% BBM group. The BBM also markedly enhanced the lysozyme activity, CD4(+)/CD8(+) T lymphocyte ratio and expression levels of IFN-γ and IL-12 in S. Typhimurium-challenged pigs. Therefore, the BBM could be a good candidate as an alternative antibiotic that improves Th 1-specific immune responses and the bacterial clearance effect.

No MeSH data available.


Related in: MedlinePlus

Histopathological features of the cecum and colon tissues in pigs challenged withS. Typhimurium infection. Representative pictures of the cecum (A,B and C) and colon (D, E and F) stained with H&E in the control group, 1% BBMgroup and 2% BBM group. In both tissues, infiltration of lymphoid cells anddesquamation of the epithelial cells are less severe in the 1% and 2% BBM groups thanin the control group. The magnification in all images is 100 ×.
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fig_004: Histopathological features of the cecum and colon tissues in pigs challenged withS. Typhimurium infection. Representative pictures of the cecum (A,B and C) and colon (D, E and F) stained with H&E in the control group, 1% BBMgroup and 2% BBM group. In both tissues, infiltration of lymphoid cells anddesquamation of the epithelial cells are less severe in the 1% and 2% BBM groups thanin the control group. The magnification in all images is 100 ×.

Mentions: Histopathological observations in Salmonella-infected pigs: All infectedpigs showed typical histopathological lesions of salmonellosis, such as severe desquamationof epithelial cells and infiltration of inflammatory cells into the mucosal layer. However,the 1% BBM group and 2% BBM group showed mild histopathological lesions in a dose-dependentmanner in the cecum and colon tissue compared with the control group (Fig. 4Fig. 4.


Improvement of bacterial clearance and relief of clinical signs of Salmonella enterica serovar Typhimurium infection in pigs through upregulation of Th 1-specific responses by administration of a combination of two silicate minerals, biotite and bentonite.

Lee JA, Jung BG, Kim TH, Kim YM, Koh HB, Lee BJ - J. Vet. Med. Sci. (2015)

Histopathological features of the cecum and colon tissues in pigs challenged withS. Typhimurium infection. Representative pictures of the cecum (A,B and C) and colon (D, E and F) stained with H&E in the control group, 1% BBMgroup and 2% BBM group. In both tissues, infiltration of lymphoid cells anddesquamation of the epithelial cells are less severe in the 1% and 2% BBM groups thanin the control group. The magnification in all images is 100 ×.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591149&req=5

fig_004: Histopathological features of the cecum and colon tissues in pigs challenged withS. Typhimurium infection. Representative pictures of the cecum (A,B and C) and colon (D, E and F) stained with H&E in the control group, 1% BBMgroup and 2% BBM group. In both tissues, infiltration of lymphoid cells anddesquamation of the epithelial cells are less severe in the 1% and 2% BBM groups thanin the control group. The magnification in all images is 100 ×.
Mentions: Histopathological observations in Salmonella-infected pigs: All infectedpigs showed typical histopathological lesions of salmonellosis, such as severe desquamationof epithelial cells and infiltration of inflammatory cells into the mucosal layer. However,the 1% BBM group and 2% BBM group showed mild histopathological lesions in a dose-dependentmanner in the cecum and colon tissue compared with the control group (Fig. 4Fig. 4.

Bottom Line: Typhimurium.Typhimurium and the number of viable bacteria in feces and tissues were significantly decreased in both BBM groups, especially in the 2% BBM group.Therefore, the BBM could be a good candidate as an alternative antibiotic that improves Th 1-specific immune responses and the bacterial clearance effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea.

ABSTRACT
Biotite and bentonite are phyllosilicate minerals that were originally used in industrial applications. Several beneficial activities of them have recently been reported, especially regulation of the immune system and antimicrobial effects. Therefore, we investigated the immune-enhancing and bacterial clearance effects of a biotite and bentonite mixture (BBM) on experimental infection of Salmonella enterica serovar Typhimurium (S. Typhimurium) to determine whether the BBM could be used as an alternative antibiotic. We administered 1% or 2% BBM as a feed supplement. We then evaluated the bacterial clearance effects of the BBM against S. Typhimurium. We also evaluated the immune-enhancing effect of the BBM through several immunological experiments that included examination of the lysozyme activity, CD4(+)/CD8(+) T lymphocyte ratio and the T-helper type 1 (Th 1) cytokine profile. The clinical signs of S. Typhimurium and the number of viable bacteria in feces and tissues were significantly decreased in both BBM groups, especially in the 2% BBM group. The BBM also markedly enhanced the lysozyme activity, CD4(+)/CD8(+) T lymphocyte ratio and expression levels of IFN-γ and IL-12 in S. Typhimurium-challenged pigs. Therefore, the BBM could be a good candidate as an alternative antibiotic that improves Th 1-specific immune responses and the bacterial clearance effect.

No MeSH data available.


Related in: MedlinePlus