Limits...
Improvement of bacterial clearance and relief of clinical signs of Salmonella enterica serovar Typhimurium infection in pigs through upregulation of Th 1-specific responses by administration of a combination of two silicate minerals, biotite and bentonite.

Lee JA, Jung BG, Kim TH, Kim YM, Koh HB, Lee BJ - J. Vet. Med. Sci. (2015)

Bottom Line: Typhimurium.Typhimurium and the number of viable bacteria in feces and tissues were significantly decreased in both BBM groups, especially in the 2% BBM group.Therefore, the BBM could be a good candidate as an alternative antibiotic that improves Th 1-specific immune responses and the bacterial clearance effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea.

ABSTRACT
Biotite and bentonite are phyllosilicate minerals that were originally used in industrial applications. Several beneficial activities of them have recently been reported, especially regulation of the immune system and antimicrobial effects. Therefore, we investigated the immune-enhancing and bacterial clearance effects of a biotite and bentonite mixture (BBM) on experimental infection of Salmonella enterica serovar Typhimurium (S. Typhimurium) to determine whether the BBM could be used as an alternative antibiotic. We administered 1% or 2% BBM as a feed supplement. We then evaluated the bacterial clearance effects of the BBM against S. Typhimurium. We also evaluated the immune-enhancing effect of the BBM through several immunological experiments that included examination of the lysozyme activity, CD4(+)/CD8(+) T lymphocyte ratio and the T-helper type 1 (Th 1) cytokine profile. The clinical signs of S. Typhimurium and the number of viable bacteria in feces and tissues were significantly decreased in both BBM groups, especially in the 2% BBM group. The BBM also markedly enhanced the lysozyme activity, CD4(+)/CD8(+) T lymphocyte ratio and expression levels of IFN-γ and IL-12 in S. Typhimurium-challenged pigs. Therefore, the BBM could be a good candidate as an alternative antibiotic that improves Th 1-specific immune responses and the bacterial clearance effect.

No MeSH data available.


Related in: MedlinePlus

Effects of the biotite and bentonite mixture (BBM) on T lymphocyte subpopulations innormal pigs. After BBM administration to pigs for 2 weeks, peripheral bloodmononuclear cells (PBMCs) were isolated, and the CD4+/CD8+ Tlymphocyte ratio was evaluated using flow cytometry. The BBM group has a significantincrease in the CD4+/CD8+ T lymphocyte ratio compared with thecontrol group. The data are presented as the mean ± SD of 5 pigs per group.**P< 0.01 vs. control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4591149&req=5

fig_001: Effects of the biotite and bentonite mixture (BBM) on T lymphocyte subpopulations innormal pigs. After BBM administration to pigs for 2 weeks, peripheral bloodmononuclear cells (PBMCs) were isolated, and the CD4+/CD8+ Tlymphocyte ratio was evaluated using flow cytometry. The BBM group has a significantincrease in the CD4+/CD8+ T lymphocyte ratio compared with thecontrol group. The data are presented as the mean ± SD of 5 pigs per group.**P< 0.01 vs. control group.

Mentions: The effects of the BBM on the T lymphocyte subpopulations in normal pigs:Flow cytometric analysis of peripheral blood was performed 14 days after administration of1% BBM or 2% BBM. The percentage of CD4+CD8− T lymphocytes in the BBMgroups significantly increased compared with those of the control group (data not shown). Inparticular, the CD4+/CD8+ T lymphocyte ratios in the BBM groups weresignificantly increased (P<0.01) compared with those observed in thecontrol group (Fig. 1Fig. 1.


Improvement of bacterial clearance and relief of clinical signs of Salmonella enterica serovar Typhimurium infection in pigs through upregulation of Th 1-specific responses by administration of a combination of two silicate minerals, biotite and bentonite.

Lee JA, Jung BG, Kim TH, Kim YM, Koh HB, Lee BJ - J. Vet. Med. Sci. (2015)

Effects of the biotite and bentonite mixture (BBM) on T lymphocyte subpopulations innormal pigs. After BBM administration to pigs for 2 weeks, peripheral bloodmononuclear cells (PBMCs) were isolated, and the CD4+/CD8+ Tlymphocyte ratio was evaluated using flow cytometry. The BBM group has a significantincrease in the CD4+/CD8+ T lymphocyte ratio compared with thecontrol group. The data are presented as the mean ± SD of 5 pigs per group.**P< 0.01 vs. control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591149&req=5

fig_001: Effects of the biotite and bentonite mixture (BBM) on T lymphocyte subpopulations innormal pigs. After BBM administration to pigs for 2 weeks, peripheral bloodmononuclear cells (PBMCs) were isolated, and the CD4+/CD8+ Tlymphocyte ratio was evaluated using flow cytometry. The BBM group has a significantincrease in the CD4+/CD8+ T lymphocyte ratio compared with thecontrol group. The data are presented as the mean ± SD of 5 pigs per group.**P< 0.01 vs. control group.
Mentions: The effects of the BBM on the T lymphocyte subpopulations in normal pigs:Flow cytometric analysis of peripheral blood was performed 14 days after administration of1% BBM or 2% BBM. The percentage of CD4+CD8− T lymphocytes in the BBMgroups significantly increased compared with those of the control group (data not shown). Inparticular, the CD4+/CD8+ T lymphocyte ratios in the BBM groups weresignificantly increased (P<0.01) compared with those observed in thecontrol group (Fig. 1Fig. 1.

Bottom Line: Typhimurium.Typhimurium and the number of viable bacteria in feces and tissues were significantly decreased in both BBM groups, especially in the 2% BBM group.Therefore, the BBM could be a good candidate as an alternative antibiotic that improves Th 1-specific immune responses and the bacterial clearance effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea.

ABSTRACT
Biotite and bentonite are phyllosilicate minerals that were originally used in industrial applications. Several beneficial activities of them have recently been reported, especially regulation of the immune system and antimicrobial effects. Therefore, we investigated the immune-enhancing and bacterial clearance effects of a biotite and bentonite mixture (BBM) on experimental infection of Salmonella enterica serovar Typhimurium (S. Typhimurium) to determine whether the BBM could be used as an alternative antibiotic. We administered 1% or 2% BBM as a feed supplement. We then evaluated the bacterial clearance effects of the BBM against S. Typhimurium. We also evaluated the immune-enhancing effect of the BBM through several immunological experiments that included examination of the lysozyme activity, CD4(+)/CD8(+) T lymphocyte ratio and the T-helper type 1 (Th 1) cytokine profile. The clinical signs of S. Typhimurium and the number of viable bacteria in feces and tissues were significantly decreased in both BBM groups, especially in the 2% BBM group. The BBM also markedly enhanced the lysozyme activity, CD4(+)/CD8(+) T lymphocyte ratio and expression levels of IFN-γ and IL-12 in S. Typhimurium-challenged pigs. Therefore, the BBM could be a good candidate as an alternative antibiotic that improves Th 1-specific immune responses and the bacterial clearance effect.

No MeSH data available.


Related in: MedlinePlus