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Characterization of novel avian paramyxovirus strain APMV/Shimane67 isolated from migratory wild geese in Japan.

Yamamoto E, Ito H, Tomioka Y, Ito T - J. Vet. Med. Sci. (2015)

Bottom Line: The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs.Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes.These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan.

ABSTRACT
An avian paramyxovirus (APMV) isolated from goose feces (APMV/Shimane67) was biologically, serologically and genetically characterized. APMV/Shimane67 showed typical paramyxovirus morphology on electron microscopy. On hemagglutination inhibition test, antiserum against APMV/Shimane67 revealed low reactivity with other APMV serotypes and vice versa. The fusion (F) protein gene of APMV/Shimane67 contained 1,638 nucleotides in a single open reading frame encoding a protein of 545 amino acids. The cleavage site of F protein contained a pair of single basic amino acid (VRENR/L). The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs. Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes. These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

No MeSH data available.


Phylogenetic tree of F gene sequences from subfamilyParamyxovirinae. The phylogenetic tree was generated using theneighbor-joining algorithm with 1,000 bootstrap replicates in Clustal X.
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fig_004: Phylogenetic tree of F gene sequences from subfamilyParamyxovirinae. The phylogenetic tree was generated using theneighbor-joining algorithm with 1,000 bootstrap replicates in Clustal X.

Mentions: Amino acid sequences of F protein cleavage site from APMVs. NDV (lentogenic); strainLaSota, NDV (velogenic); strain Herts 33, APMV-4/HK/D3; APMV-4/duck/Hong Kong/D3/1975,APMV-5/Kunitachi; APMV-5/budgerigar/ Japan/Kunitachi/1974, APMV-6/TWN/Y1;APMV-6/duck/Taiwan/Y1/1998, APMV-8/DE; APMV-8/goose/Delaware/1053/1976, APMV-9/NY;duck/New York/22/1978, APMV-10/FLK/324; APMV10/penguin/Falkland Islands/324/2007,APMV-11/FRA/100212; APMV-11/common_snipe/France/100212/2010. Abbreviations of otherAPMV are given in the text.


Characterization of novel avian paramyxovirus strain APMV/Shimane67 isolated from migratory wild geese in Japan.

Yamamoto E, Ito H, Tomioka Y, Ito T - J. Vet. Med. Sci. (2015)

Phylogenetic tree of F gene sequences from subfamilyParamyxovirinae. The phylogenetic tree was generated using theneighbor-joining algorithm with 1,000 bootstrap replicates in Clustal X.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591148&req=5

fig_004: Phylogenetic tree of F gene sequences from subfamilyParamyxovirinae. The phylogenetic tree was generated using theneighbor-joining algorithm with 1,000 bootstrap replicates in Clustal X.
Mentions: Amino acid sequences of F protein cleavage site from APMVs. NDV (lentogenic); strainLaSota, NDV (velogenic); strain Herts 33, APMV-4/HK/D3; APMV-4/duck/Hong Kong/D3/1975,APMV-5/Kunitachi; APMV-5/budgerigar/ Japan/Kunitachi/1974, APMV-6/TWN/Y1;APMV-6/duck/Taiwan/Y1/1998, APMV-8/DE; APMV-8/goose/Delaware/1053/1976, APMV-9/NY;duck/New York/22/1978, APMV-10/FLK/324; APMV10/penguin/Falkland Islands/324/2007,APMV-11/FRA/100212; APMV-11/common_snipe/France/100212/2010. Abbreviations of otherAPMV are given in the text.

Bottom Line: The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs.Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes.These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan.

ABSTRACT
An avian paramyxovirus (APMV) isolated from goose feces (APMV/Shimane67) was biologically, serologically and genetically characterized. APMV/Shimane67 showed typical paramyxovirus morphology on electron microscopy. On hemagglutination inhibition test, antiserum against APMV/Shimane67 revealed low reactivity with other APMV serotypes and vice versa. The fusion (F) protein gene of APMV/Shimane67 contained 1,638 nucleotides in a single open reading frame encoding a protein of 545 amino acids. The cleavage site of F protein contained a pair of single basic amino acid (VRENR/L). The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs. Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes. These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

No MeSH data available.