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Characterization of novel avian paramyxovirus strain APMV/Shimane67 isolated from migratory wild geese in Japan.

Yamamoto E, Ito H, Tomioka Y, Ito T - J. Vet. Med. Sci. (2015)

Bottom Line: The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs.Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes.These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan.

ABSTRACT
An avian paramyxovirus (APMV) isolated from goose feces (APMV/Shimane67) was biologically, serologically and genetically characterized. APMV/Shimane67 showed typical paramyxovirus morphology on electron microscopy. On hemagglutination inhibition test, antiserum against APMV/Shimane67 revealed low reactivity with other APMV serotypes and vice versa. The fusion (F) protein gene of APMV/Shimane67 contained 1,638 nucleotides in a single open reading frame encoding a protein of 545 amino acids. The cleavage site of F protein contained a pair of single basic amino acid (VRENR/L). The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs. Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes. These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

No MeSH data available.


Related in: MedlinePlus

Amino acid sequences of F protein cleavage site from APMVs. NDV (lentogenic); strainLaSota, NDV (velogenic); strain Herts 33, APMV-4/HK/D3; APMV-4/duck/Hong Kong/D3/1975,APMV-5/Kunitachi; APMV-5/budgerigar/ Japan/Kunitachi/1974, APMV-6/TWN/Y1;APMV-6/duck/Taiwan/Y1/1998, APMV-8/DE; APMV-8/goose/Delaware/1053/1976, APMV-9/NY;duck/New York/22/1978, APMV-10/FLK/324; APMV10/penguin/Falkland Islands/324/2007,APMV-11/FRA/100212; APMV-11/common_snipe/France/100212/2010. Abbreviations of otherAPMV are given in the text.
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fig_003: Amino acid sequences of F protein cleavage site from APMVs. NDV (lentogenic); strainLaSota, NDV (velogenic); strain Herts 33, APMV-4/HK/D3; APMV-4/duck/Hong Kong/D3/1975,APMV-5/Kunitachi; APMV-5/budgerigar/ Japan/Kunitachi/1974, APMV-6/TWN/Y1;APMV-6/duck/Taiwan/Y1/1998, APMV-8/DE; APMV-8/goose/Delaware/1053/1976, APMV-9/NY;duck/New York/22/1978, APMV-10/FLK/324; APMV10/penguin/Falkland Islands/324/2007,APMV-11/FRA/100212; APMV-11/common_snipe/France/100212/2010. Abbreviations of otherAPMV are given in the text.

Mentions: Nucleotide sequencing and phylogenetic analysis: A total of 1,878nucleotides of the genome sequence of APMV/Shimane67 were determined. The putativeinitiation codon, which better matched the Kozak consensus sequence ((G/A) NNATGG) [8], was the third ATG from 5′-end of the F10 primerbinding site (data not shown). It contained a long open reading frame (ORF) composed of1,638 nucleotides and encoded 545 amino acids. The nucleotide sequence of this ORF showedsimilarities with the F genes of NDVs and APMV-12/wigeon/Italy/3920_1/2005 (APMV-12/ITA) bydiscontiguous MegaBLAST search [24], indicating thatthis ORF was the F gene of APMV/Shimane67. The nucleotide and deduced amino acid identitiesbetween the F gene of APMV/Shimane67 and other APMV serotypes ranged from 42.9% withAPMV-3/NLD to 62.7% with APMV-12/ITA, and from 28.9% with APMV-3/NLD to 67.3% withAPMV-12/ITA, respectively (Table 3Table 3.Nucleotide (upper right) and deduced amino acid (lower left) identities (%) amongthe fusion protein gene of avian paramyxovirusesVirusShimane 67NDVAPMV-2APMV-3APMV-4APMV-5APMV-6APMV-7APMV-8APMV-9APMV-10APMV-11APMV-12AK/ 415LaSotaSF02YucaipaBangorNLDWIHK/D3KR/YJKunitachiTWN/Y1ITA/ 4524-2TNDE/1053WakuyaNY/22ITA/5709FLK/324FRA/ 100212ITA/ 3920_1APMV/Shimane67-53.656.154.345.644.642.943.043.743.346.244.645.343.546.646.853.353.446.343.862.7NDV/AK/41552.8-71.871.546.446.843.341.042.842.054.444.645.044.747.047.458.558.445.643.155.1NDV/LaSota53.787.3-84.447.546.642.341.941.641.446.844.445.043.546.746.958.260.346.142.155.5NDV/SF0252.684.888.6-47.548.042.542.242.741.745.344.944.444.647.347.358.458.745.543.254.8APMV-2/Yucaipa37.340.440.640.4-69.840.741.142.841.850.250.951.847.260.560.946.347.259.248.546.7APMV-2/Bangor36.540.741.341.379.1-41.041.542.441.850.451.152.348.360.560.046.146.559.850.046.1APMV-3/NLD28.931.231.230.730.831.5-66.847.847.442.342.742.441.343.443.240.740.140.842.043.2APMV-3/WI29.430.030.230.030.030.370.1-46.847.143.242.342.642.541.641.741.441.241.544.341.6APMV-4/HK/D332.331.031.331.033.634.032.033.5-92.343.542.943.540.844.244.141.942.443.341.940.9APMV-4/KR/YJ32.130.831.330.633.634.232.233.197.7-42.543.144.040.644.143.741.142.442.441.041.3APMV-5/Kunitachi39.340.141.041.446.545.731.030.933.933.5-56.557.047.950.550.744.945.052.146.944.6APMV-6/TWN/Y136.936.837.837.848.849.431.629.833.633.053.7-72.647.349.349.445.144.952.348.945.6APMV-6/ITA/4524-237.337.137.437.649.248.631.229.533.833.654.185.7-47.050.450.044.84552.249.245.1APMV-7/TN35.738.13938.838.138.828.530.130.129.338.037.036.9-49.249.042.843.748.853.044.4APMV-8/DE/105336.440.141.141.163.763.731.830.635.935.945.548.247.338.3-97.147.347.659.548.246.8APMV-8/Wakuya36.240.141.141.163.763.731.830.635.935.745.548.247.338.199.4-47.548.059.548.446.4APMV-9/NY/2248.456.455.956.437.839.128.228.328.428.636.537.436.635.637.937.9-89.245.542.854.3APMV-9/ITA/570950.158.158.658.437.839.629.329.228.628.837.837.937.536.639.439.492.7-46.542.655.2APMV-10/FLK/32437.839.539.939.561.760.329.028.132.432.246.348.148.038.261.461.438.939.1-48.046.5APMV-11/FRA/10021231.632.633.933.942.441.831.033.032.532.239.939.741.736.040.440.432.733.339.4-43.9APMV-12/ITA/3920_167.355.154.653.838.137.329.528.532.131.937.437.737.936.536.136.152.353.237.832.7-). The deduced amino acid sequence of the putative cleavage site of theAPMV/Shimane F gene was QVRENR/LVG (Fig. 3Fig. 3.


Characterization of novel avian paramyxovirus strain APMV/Shimane67 isolated from migratory wild geese in Japan.

Yamamoto E, Ito H, Tomioka Y, Ito T - J. Vet. Med. Sci. (2015)

Amino acid sequences of F protein cleavage site from APMVs. NDV (lentogenic); strainLaSota, NDV (velogenic); strain Herts 33, APMV-4/HK/D3; APMV-4/duck/Hong Kong/D3/1975,APMV-5/Kunitachi; APMV-5/budgerigar/ Japan/Kunitachi/1974, APMV-6/TWN/Y1;APMV-6/duck/Taiwan/Y1/1998, APMV-8/DE; APMV-8/goose/Delaware/1053/1976, APMV-9/NY;duck/New York/22/1978, APMV-10/FLK/324; APMV10/penguin/Falkland Islands/324/2007,APMV-11/FRA/100212; APMV-11/common_snipe/France/100212/2010. Abbreviations of otherAPMV are given in the text.
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Related In: Results  -  Collection

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fig_003: Amino acid sequences of F protein cleavage site from APMVs. NDV (lentogenic); strainLaSota, NDV (velogenic); strain Herts 33, APMV-4/HK/D3; APMV-4/duck/Hong Kong/D3/1975,APMV-5/Kunitachi; APMV-5/budgerigar/ Japan/Kunitachi/1974, APMV-6/TWN/Y1;APMV-6/duck/Taiwan/Y1/1998, APMV-8/DE; APMV-8/goose/Delaware/1053/1976, APMV-9/NY;duck/New York/22/1978, APMV-10/FLK/324; APMV10/penguin/Falkland Islands/324/2007,APMV-11/FRA/100212; APMV-11/common_snipe/France/100212/2010. Abbreviations of otherAPMV are given in the text.
Mentions: Nucleotide sequencing and phylogenetic analysis: A total of 1,878nucleotides of the genome sequence of APMV/Shimane67 were determined. The putativeinitiation codon, which better matched the Kozak consensus sequence ((G/A) NNATGG) [8], was the third ATG from 5′-end of the F10 primerbinding site (data not shown). It contained a long open reading frame (ORF) composed of1,638 nucleotides and encoded 545 amino acids. The nucleotide sequence of this ORF showedsimilarities with the F genes of NDVs and APMV-12/wigeon/Italy/3920_1/2005 (APMV-12/ITA) bydiscontiguous MegaBLAST search [24], indicating thatthis ORF was the F gene of APMV/Shimane67. The nucleotide and deduced amino acid identitiesbetween the F gene of APMV/Shimane67 and other APMV serotypes ranged from 42.9% withAPMV-3/NLD to 62.7% with APMV-12/ITA, and from 28.9% with APMV-3/NLD to 67.3% withAPMV-12/ITA, respectively (Table 3Table 3.Nucleotide (upper right) and deduced amino acid (lower left) identities (%) amongthe fusion protein gene of avian paramyxovirusesVirusShimane 67NDVAPMV-2APMV-3APMV-4APMV-5APMV-6APMV-7APMV-8APMV-9APMV-10APMV-11APMV-12AK/ 415LaSotaSF02YucaipaBangorNLDWIHK/D3KR/YJKunitachiTWN/Y1ITA/ 4524-2TNDE/1053WakuyaNY/22ITA/5709FLK/324FRA/ 100212ITA/ 3920_1APMV/Shimane67-53.656.154.345.644.642.943.043.743.346.244.645.343.546.646.853.353.446.343.862.7NDV/AK/41552.8-71.871.546.446.843.341.042.842.054.444.645.044.747.047.458.558.445.643.155.1NDV/LaSota53.787.3-84.447.546.642.341.941.641.446.844.445.043.546.746.958.260.346.142.155.5NDV/SF0252.684.888.6-47.548.042.542.242.741.745.344.944.444.647.347.358.458.745.543.254.8APMV-2/Yucaipa37.340.440.640.4-69.840.741.142.841.850.250.951.847.260.560.946.347.259.248.546.7APMV-2/Bangor36.540.741.341.379.1-41.041.542.441.850.451.152.348.360.560.046.146.559.850.046.1APMV-3/NLD28.931.231.230.730.831.5-66.847.847.442.342.742.441.343.443.240.740.140.842.043.2APMV-3/WI29.430.030.230.030.030.370.1-46.847.143.242.342.642.541.641.741.441.241.544.341.6APMV-4/HK/D332.331.031.331.033.634.032.033.5-92.343.542.943.540.844.244.141.942.443.341.940.9APMV-4/KR/YJ32.130.831.330.633.634.232.233.197.7-42.543.144.040.644.143.741.142.442.441.041.3APMV-5/Kunitachi39.340.141.041.446.545.731.030.933.933.5-56.557.047.950.550.744.945.052.146.944.6APMV-6/TWN/Y136.936.837.837.848.849.431.629.833.633.053.7-72.647.349.349.445.144.952.348.945.6APMV-6/ITA/4524-237.337.137.437.649.248.631.229.533.833.654.185.7-47.050.450.044.84552.249.245.1APMV-7/TN35.738.13938.838.138.828.530.130.129.338.037.036.9-49.249.042.843.748.853.044.4APMV-8/DE/105336.440.141.141.163.763.731.830.635.935.945.548.247.338.3-97.147.347.659.548.246.8APMV-8/Wakuya36.240.141.141.163.763.731.830.635.935.745.548.247.338.199.4-47.548.059.548.446.4APMV-9/NY/2248.456.455.956.437.839.128.228.328.428.636.537.436.635.637.937.9-89.245.542.854.3APMV-9/ITA/570950.158.158.658.437.839.629.329.228.628.837.837.937.536.639.439.492.7-46.542.655.2APMV-10/FLK/32437.839.539.939.561.760.329.028.132.432.246.348.148.038.261.461.438.939.1-48.046.5APMV-11/FRA/10021231.632.633.933.942.441.831.033.032.532.239.939.741.736.040.440.432.733.339.4-43.9APMV-12/ITA/3920_167.355.154.653.838.137.329.528.532.131.937.437.737.936.536.136.152.353.237.832.7-). The deduced amino acid sequence of the putative cleavage site of theAPMV/Shimane F gene was QVRENR/LVG (Fig. 3Fig. 3.

Bottom Line: The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs.Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes.These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan.

ABSTRACT
An avian paramyxovirus (APMV) isolated from goose feces (APMV/Shimane67) was biologically, serologically and genetically characterized. APMV/Shimane67 showed typical paramyxovirus morphology on electron microscopy. On hemagglutination inhibition test, antiserum against APMV/Shimane67 revealed low reactivity with other APMV serotypes and vice versa. The fusion (F) protein gene of APMV/Shimane67 contained 1,638 nucleotides in a single open reading frame encoding a protein of 545 amino acids. The cleavage site of F protein contained a pair of single basic amino acid (VRENR/L). The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs. Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes. These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

No MeSH data available.


Related in: MedlinePlus