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Characterization of novel avian paramyxovirus strain APMV/Shimane67 isolated from migratory wild geese in Japan.

Yamamoto E, Ito H, Tomioka Y, Ito T - J. Vet. Med. Sci. (2015)

Bottom Line: The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs.Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes.These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan.

ABSTRACT
An avian paramyxovirus (APMV) isolated from goose feces (APMV/Shimane67) was biologically, serologically and genetically characterized. APMV/Shimane67 showed typical paramyxovirus morphology on electron microscopy. On hemagglutination inhibition test, antiserum against APMV/Shimane67 revealed low reactivity with other APMV serotypes and vice versa. The fusion (F) protein gene of APMV/Shimane67 contained 1,638 nucleotides in a single open reading frame encoding a protein of 545 amino acids. The cleavage site of F protein contained a pair of single basic amino acid (VRENR/L). The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs. Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes. These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

No MeSH data available.


MDBK cells infected with APMV/Shimane67.
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fig_002: MDBK cells infected with APMV/Shimane67.

Mentions: Growth in cell culture and pathogenicity of APMV/Shimane67: Growth ofAPMV/Shimane67 in avian and mammalian origin cell lines (DF-1, MDCK, MDBK, BHK, Vero and 293cells) was measured. In the presence of trypsin, regardless of cell lines, APMV/Shimane67efficiently grew and reached approximately 105.0 EID50/0.1ml at 72 hr post-infection. Syncytium formation, a typical characteristicof paramyxovirus cytopathic effects, was clearly observed, particularly in MDBK cells (Fig. 2Fig. 2.


Characterization of novel avian paramyxovirus strain APMV/Shimane67 isolated from migratory wild geese in Japan.

Yamamoto E, Ito H, Tomioka Y, Ito T - J. Vet. Med. Sci. (2015)

MDBK cells infected with APMV/Shimane67.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591148&req=5

fig_002: MDBK cells infected with APMV/Shimane67.
Mentions: Growth in cell culture and pathogenicity of APMV/Shimane67: Growth ofAPMV/Shimane67 in avian and mammalian origin cell lines (DF-1, MDCK, MDBK, BHK, Vero and 293cells) was measured. In the presence of trypsin, regardless of cell lines, APMV/Shimane67efficiently grew and reached approximately 105.0 EID50/0.1ml at 72 hr post-infection. Syncytium formation, a typical characteristicof paramyxovirus cytopathic effects, was clearly observed, particularly in MDBK cells (Fig. 2Fig. 2.

Bottom Line: The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs.Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes.These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan.

ABSTRACT
An avian paramyxovirus (APMV) isolated from goose feces (APMV/Shimane67) was biologically, serologically and genetically characterized. APMV/Shimane67 showed typical paramyxovirus morphology on electron microscopy. On hemagglutination inhibition test, antiserum against APMV/Shimane67 revealed low reactivity with other APMV serotypes and vice versa. The fusion (F) protein gene of APMV/Shimane67 contained 1,638 nucleotides in a single open reading frame encoding a protein of 545 amino acids. The cleavage site of F protein contained a pair of single basic amino acid (VRENR/L). The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs. Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes. These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

No MeSH data available.