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Characterization of novel avian paramyxovirus strain APMV/Shimane67 isolated from migratory wild geese in Japan.

Yamamoto E, Ito H, Tomioka Y, Ito T - J. Vet. Med. Sci. (2015)

Bottom Line: The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs.Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes.These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan.

ABSTRACT
An avian paramyxovirus (APMV) isolated from goose feces (APMV/Shimane67) was biologically, serologically and genetically characterized. APMV/Shimane67 showed typical paramyxovirus morphology on electron microscopy. On hemagglutination inhibition test, antiserum against APMV/Shimane67 revealed low reactivity with other APMV serotypes and vice versa. The fusion (F) protein gene of APMV/Shimane67 contained 1,638 nucleotides in a single open reading frame encoding a protein of 545 amino acids. The cleavage site of F protein contained a pair of single basic amino acid (VRENR/L). The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs. Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes. These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

No MeSH data available.


Related in: MedlinePlus

Negative contrast electron micrograph of APMV/Shimane67 virus showing a partiallydisrupted particle with nucleocapsid emerging. × 200,000.
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fig_001: Negative contrast electron micrograph of APMV/Shimane67 virus showing a partiallydisrupted particle with nucleocapsid emerging. × 200,000.

Mentions: Electron microscopy: Electron microscopy of APMV/Shimane67 showed typicalcharacteristics of a paramyxovirus. Virions were spherical or pleomorphic, envelopedparticles (250–300 nm in diameter), and virion surfaces were covered with spiked projectionsof about 8–10 nm in length (Fig. 1Fig. 1.


Characterization of novel avian paramyxovirus strain APMV/Shimane67 isolated from migratory wild geese in Japan.

Yamamoto E, Ito H, Tomioka Y, Ito T - J. Vet. Med. Sci. (2015)

Negative contrast electron micrograph of APMV/Shimane67 virus showing a partiallydisrupted particle with nucleocapsid emerging. × 200,000.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591148&req=5

fig_001: Negative contrast electron micrograph of APMV/Shimane67 virus showing a partiallydisrupted particle with nucleocapsid emerging. × 200,000.
Mentions: Electron microscopy: Electron microscopy of APMV/Shimane67 showed typicalcharacteristics of a paramyxovirus. Virions were spherical or pleomorphic, envelopedparticles (250–300 nm in diameter), and virion surfaces were covered with spiked projectionsof about 8–10 nm in length (Fig. 1Fig. 1.

Bottom Line: The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs.Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes.These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

View Article: PubMed Central - PubMed

Affiliation: United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan.

ABSTRACT
An avian paramyxovirus (APMV) isolated from goose feces (APMV/Shimane67) was biologically, serologically and genetically characterized. APMV/Shimane67 showed typical paramyxovirus morphology on electron microscopy. On hemagglutination inhibition test, antiserum against APMV/Shimane67 revealed low reactivity with other APMV serotypes and vice versa. The fusion (F) protein gene of APMV/Shimane67 contained 1,638 nucleotides in a single open reading frame encoding a protein of 545 amino acids. The cleavage site of F protein contained a pair of single basic amino acid (VRENR/L). The nucleotide and deduced amino acid sequences of the F gene of APMV/Shimane67 had relatively low identities (42.9-62.7% and 28.9-67.3%, respectively) with those of other APMVs. Phylogenetic analysis showed that APMV/Shimane67 was related to NDV, APMV-9 and APMV-12, but was distinct from those APMV serotypes. These results suggest that APMV/Shimane67 is a new APMV serotype, APMV-13.

No MeSH data available.


Related in: MedlinePlus