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Suppression of influenza virus infection by the orf virus isolated in Taiwan.

Lin FY, Tseng YY, Chan KW, Kuo ST, Yang CH, Wang CY, Takasu M, Hsu WL, Wong ML - J. Vet. Med. Sci. (2015)

Bottom Line: ORFV stimulated human monocytes (THP-1) secreting proinflammatory cytokines IL-8 and TNF-α.Similarly, mice infected with ORFV via both intramuscular and subcutaneous routes at two days prior to IAV infection significantly decreased the replication of IAV.In summary, the results of a current study indicated our Hoping strain harbors the immune modulator property; with such a bio-adjuvanticity, we further proved that pre-exposure of ORFV protects animals from subsequent IAV infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan.

ABSTRACT
Orf virus (ORFV), a member of parapoxvirus, is an enveloped virus with genome of double-stranded DNA. ORFV causes contagious pustular dermatitis or contagious ecthyma in sheep and goats worldwide. In general, detection of viral DNA and observing ORFV virion in tissues of afflicted animals are two methods commonly used for diagnosis of orf infection; however, isolation of the ORFV in cell culture using virus-containing tissue as inoculum is known to be difficult. In this work, the ORFV (Hoping strain) isolated in central Taiwan was successfully grown in cell culture. We further examined the biochemical characteristic of our isolate, including viral genotyping, viral mRNA and protein expression. By electron microscopy, one unique form of viral particle from ORFV infected cellular lysate was demonstrated in the negative-stained field. Moreover, immunomodulating and anti-influenza virus properties of this ORFV were investigated. ORFV stimulated human monocytes (THP-1) secreting proinflammatory cytokines IL-8 and TNF-α. And, pre-treatment of ORFV-infected cell medium prevents A549 cells from subsequent type A influenza virus (IAV) infection. Similarly, mice infected with ORFV via both intramuscular and subcutaneous routes at two days prior to IAV infection significantly decreased the replication of IAV. In summary, the results of a current study indicated our Hoping strain harbors the immune modulator property; with such a bio-adjuvanticity, we further proved that pre-exposure of ORFV protects animals from subsequent IAV infection.

No MeSH data available.


Related in: MedlinePlus

Infection of ORFV suppresses the subsequent IAV infection in mice. ORFV wasadministrated intra-muscularly (IM) or subcutaneously (SC) to BALB/c mice (6 weeksold, n=4) at dosage of 2 × 105 PFU before IAV infection. One group wasadministrated with as a control (n=4). Two days later, all groups of mice received adosage of 1 × 104 PFU IAV via intra-nasal route. Mice were observed tocheck any illness symptoms. Seven days post infection, all the mice were sacrificed,and their lungs were removed for titration of the virus loads (A), and IL-6 and TNF-αexpressions in blood were evaluated by ELISA (BioLegend) (B). Values display the means(+/–SD) of each group. Statistical analysis was performed using unpaired T-test withWelch’s correction. P value<0.05, shown with a star symbol,indicates statistical difference between 2 groups.
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fig_005: Infection of ORFV suppresses the subsequent IAV infection in mice. ORFV wasadministrated intra-muscularly (IM) or subcutaneously (SC) to BALB/c mice (6 weeksold, n=4) at dosage of 2 × 105 PFU before IAV infection. One group wasadministrated with as a control (n=4). Two days later, all groups of mice received adosage of 1 × 104 PFU IAV via intra-nasal route. Mice were observed tocheck any illness symptoms. Seven days post infection, all the mice were sacrificed,and their lungs were removed for titration of the virus loads (A), and IL-6 and TNF-αexpressions in blood were evaluated by ELISA (BioLegend) (B). Values display the means(+/–SD) of each group. Statistical analysis was performed using unpaired T-test withWelch’s correction. P value<0.05, shown with a star symbol,indicates statistical difference between 2 groups.

Mentions: Inhibition of subsequent influenza virus replication in mice inoculated withUV-inactivated ORFV: To further investigate the inhibitory activity on influenzavirus infection by UV-inactivated ORFV, 6 weeks old female BALB/C mice were inoculated witheither 2 × 105 PFU of UV-inactivated ORFV (n=4) or PBS (n=4, as a negativecontrol) by IM and SC routes for 2 days and subsequently infected with influenza virus. Asshown in Fig. 5AFig. 5.


Suppression of influenza virus infection by the orf virus isolated in Taiwan.

Lin FY, Tseng YY, Chan KW, Kuo ST, Yang CH, Wang CY, Takasu M, Hsu WL, Wong ML - J. Vet. Med. Sci. (2015)

Infection of ORFV suppresses the subsequent IAV infection in mice. ORFV wasadministrated intra-muscularly (IM) or subcutaneously (SC) to BALB/c mice (6 weeksold, n=4) at dosage of 2 × 105 PFU before IAV infection. One group wasadministrated with as a control (n=4). Two days later, all groups of mice received adosage of 1 × 104 PFU IAV via intra-nasal route. Mice were observed tocheck any illness symptoms. Seven days post infection, all the mice were sacrificed,and their lungs were removed for titration of the virus loads (A), and IL-6 and TNF-αexpressions in blood were evaluated by ELISA (BioLegend) (B). Values display the means(+/–SD) of each group. Statistical analysis was performed using unpaired T-test withWelch’s correction. P value<0.05, shown with a star symbol,indicates statistical difference between 2 groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591145&req=5

fig_005: Infection of ORFV suppresses the subsequent IAV infection in mice. ORFV wasadministrated intra-muscularly (IM) or subcutaneously (SC) to BALB/c mice (6 weeksold, n=4) at dosage of 2 × 105 PFU before IAV infection. One group wasadministrated with as a control (n=4). Two days later, all groups of mice received adosage of 1 × 104 PFU IAV via intra-nasal route. Mice were observed tocheck any illness symptoms. Seven days post infection, all the mice were sacrificed,and their lungs were removed for titration of the virus loads (A), and IL-6 and TNF-αexpressions in blood were evaluated by ELISA (BioLegend) (B). Values display the means(+/–SD) of each group. Statistical analysis was performed using unpaired T-test withWelch’s correction. P value<0.05, shown with a star symbol,indicates statistical difference between 2 groups.
Mentions: Inhibition of subsequent influenza virus replication in mice inoculated withUV-inactivated ORFV: To further investigate the inhibitory activity on influenzavirus infection by UV-inactivated ORFV, 6 weeks old female BALB/C mice were inoculated witheither 2 × 105 PFU of UV-inactivated ORFV (n=4) or PBS (n=4, as a negativecontrol) by IM and SC routes for 2 days and subsequently infected with influenza virus. Asshown in Fig. 5AFig. 5.

Bottom Line: ORFV stimulated human monocytes (THP-1) secreting proinflammatory cytokines IL-8 and TNF-α.Similarly, mice infected with ORFV via both intramuscular and subcutaneous routes at two days prior to IAV infection significantly decreased the replication of IAV.In summary, the results of a current study indicated our Hoping strain harbors the immune modulator property; with such a bio-adjuvanticity, we further proved that pre-exposure of ORFV protects animals from subsequent IAV infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan.

ABSTRACT
Orf virus (ORFV), a member of parapoxvirus, is an enveloped virus with genome of double-stranded DNA. ORFV causes contagious pustular dermatitis or contagious ecthyma in sheep and goats worldwide. In general, detection of viral DNA and observing ORFV virion in tissues of afflicted animals are two methods commonly used for diagnosis of orf infection; however, isolation of the ORFV in cell culture using virus-containing tissue as inoculum is known to be difficult. In this work, the ORFV (Hoping strain) isolated in central Taiwan was successfully grown in cell culture. We further examined the biochemical characteristic of our isolate, including viral genotyping, viral mRNA and protein expression. By electron microscopy, one unique form of viral particle from ORFV infected cellular lysate was demonstrated in the negative-stained field. Moreover, immunomodulating and anti-influenza virus properties of this ORFV were investigated. ORFV stimulated human monocytes (THP-1) secreting proinflammatory cytokines IL-8 and TNF-α. And, pre-treatment of ORFV-infected cell medium prevents A549 cells from subsequent type A influenza virus (IAV) infection. Similarly, mice infected with ORFV via both intramuscular and subcutaneous routes at two days prior to IAV infection significantly decreased the replication of IAV. In summary, the results of a current study indicated our Hoping strain harbors the immune modulator property; with such a bio-adjuvanticity, we further proved that pre-exposure of ORFV protects animals from subsequent IAV infection.

No MeSH data available.


Related in: MedlinePlus