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Characterization of Aerococcus viridans isolated from milk samples from cows with mastitis and manure samples.

Saishu N, Morimoto K, Yamasato H, Ozaki H, Murase T - J. Vet. Med. Sci. (2015)

Bottom Line: Antimicrobial susceptibilities were determined for 29 isolates from milk, processed manure and bedding materials.Of these, 26 (89.7%) were resistant to clindamycin, whereas virtually all the isolates were susceptible to 12 other antimicrobials including cefalosporins that have been used to treat bovine mastitis in Japan.In vitro, three A. viridans isolates from milk and an isolate from processed manure survived for 3 hr in Good's buffer (pH 9) at high temperature (50°C).

View Article: PubMed Central - PubMed

Affiliation: Tottori Prefectural Kurayoshi Livestock Hygiene Service Center, 2-132 Seidani-cho, Kurayoshi, Tottori 682-0017, Japan.

ABSTRACT
Thirty-eight Aerococcus viridans isolates were obtained from milk from 478 cows with clinical mastitis in a farm during the periods between November 2011 and February 2012, and between December 2012 and March 2013. Additional isolates were obtained from processed manure (a mixture of composted manure, straw and hydrated lime) and bedding materials. The processed manure was later used to cover the floor of the stalls in barns as bedding materials. The temperatures recorded in the composted and processed manure were not as high as those generally observed during satisfactory composting. To reveal the association of A. viridans in manure-related products with intramammary infection in cows, isolates were characterized by their DNA fragment patterns as determined by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Isolates obtained from milk, processed manure and bedding materials had identical DNA fragment patterns. Antimicrobial susceptibilities were determined for 29 isolates from milk, processed manure and bedding materials. Of these, 26 (89.7%) were resistant to clindamycin, whereas virtually all the isolates were susceptible to 12 other antimicrobials including cefalosporins that have been used to treat bovine mastitis in Japan. In vitro, three A. viridans isolates from milk and an isolate from processed manure survived for 3 hr in Good's buffer (pH 9) at high temperature (50°C). The results suggest that the processed manure and bedding materials in this farm were possible sources of A. viridans that caused infection in the cows with mastitis.

No MeSH data available.


Related in: MedlinePlus

Changes in viable cell counts of A. viridans and E.coli in (A) PBS (pH 7) and (B) the Good’s buffer (pH 9) at 50°C after 0(solid column), 3 (grey), 6 (grid) and 24 (open) hr. A. viridansisolates #6, #9, #19 and #23 correspond to those with the same numbers in Fig. 2. E. coli isolates A andB were from milk samples of cows with mastitis. 700406 and 25922 represent the ATCCstrains of A. viridans and E. coli, respectively.Dashed lines indicate the detection limit (3.0 × 102cfu/ml). Significant differences between initial viable cellnumbers and those post inoculation are indicated by asterisks.
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fig_003: Changes in viable cell counts of A. viridans and E.coli in (A) PBS (pH 7) and (B) the Good’s buffer (pH 9) at 50°C after 0(solid column), 3 (grey), 6 (grid) and 24 (open) hr. A. viridansisolates #6, #9, #19 and #23 correspond to those with the same numbers in Fig. 2. E. coli isolates A andB were from milk samples of cows with mastitis. 700406 and 25922 represent the ATCCstrains of A. viridans and E. coli, respectively.Dashed lines indicate the detection limit (3.0 × 102cfu/ml). Significant differences between initial viable cellnumbers and those post inoculation are indicated by asterisks.

Mentions: Survival of A. viridans at high pH and high temperature: Three milkstrains (Fig. 2, lanes 6, 9 and 19) and a strainobtained from bedding materials (lane 23) were used. At pH 7 (Fig. 3AFig. 3.


Characterization of Aerococcus viridans isolated from milk samples from cows with mastitis and manure samples.

Saishu N, Morimoto K, Yamasato H, Ozaki H, Murase T - J. Vet. Med. Sci. (2015)

Changes in viable cell counts of A. viridans and E.coli in (A) PBS (pH 7) and (B) the Good’s buffer (pH 9) at 50°C after 0(solid column), 3 (grey), 6 (grid) and 24 (open) hr. A. viridansisolates #6, #9, #19 and #23 correspond to those with the same numbers in Fig. 2. E. coli isolates A andB were from milk samples of cows with mastitis. 700406 and 25922 represent the ATCCstrains of A. viridans and E. coli, respectively.Dashed lines indicate the detection limit (3.0 × 102cfu/ml). Significant differences between initial viable cellnumbers and those post inoculation are indicated by asterisks.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591142&req=5

fig_003: Changes in viable cell counts of A. viridans and E.coli in (A) PBS (pH 7) and (B) the Good’s buffer (pH 9) at 50°C after 0(solid column), 3 (grey), 6 (grid) and 24 (open) hr. A. viridansisolates #6, #9, #19 and #23 correspond to those with the same numbers in Fig. 2. E. coli isolates A andB were from milk samples of cows with mastitis. 700406 and 25922 represent the ATCCstrains of A. viridans and E. coli, respectively.Dashed lines indicate the detection limit (3.0 × 102cfu/ml). Significant differences between initial viable cellnumbers and those post inoculation are indicated by asterisks.
Mentions: Survival of A. viridans at high pH and high temperature: Three milkstrains (Fig. 2, lanes 6, 9 and 19) and a strainobtained from bedding materials (lane 23) were used. At pH 7 (Fig. 3AFig. 3.

Bottom Line: Antimicrobial susceptibilities were determined for 29 isolates from milk, processed manure and bedding materials.Of these, 26 (89.7%) were resistant to clindamycin, whereas virtually all the isolates were susceptible to 12 other antimicrobials including cefalosporins that have been used to treat bovine mastitis in Japan.In vitro, three A. viridans isolates from milk and an isolate from processed manure survived for 3 hr in Good's buffer (pH 9) at high temperature (50°C).

View Article: PubMed Central - PubMed

Affiliation: Tottori Prefectural Kurayoshi Livestock Hygiene Service Center, 2-132 Seidani-cho, Kurayoshi, Tottori 682-0017, Japan.

ABSTRACT
Thirty-eight Aerococcus viridans isolates were obtained from milk from 478 cows with clinical mastitis in a farm during the periods between November 2011 and February 2012, and between December 2012 and March 2013. Additional isolates were obtained from processed manure (a mixture of composted manure, straw and hydrated lime) and bedding materials. The processed manure was later used to cover the floor of the stalls in barns as bedding materials. The temperatures recorded in the composted and processed manure were not as high as those generally observed during satisfactory composting. To reveal the association of A. viridans in manure-related products with intramammary infection in cows, isolates were characterized by their DNA fragment patterns as determined by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Isolates obtained from milk, processed manure and bedding materials had identical DNA fragment patterns. Antimicrobial susceptibilities were determined for 29 isolates from milk, processed manure and bedding materials. Of these, 26 (89.7%) were resistant to clindamycin, whereas virtually all the isolates were susceptible to 12 other antimicrobials including cefalosporins that have been used to treat bovine mastitis in Japan. In vitro, three A. viridans isolates from milk and an isolate from processed manure survived for 3 hr in Good's buffer (pH 9) at high temperature (50°C). The results suggest that the processed manure and bedding materials in this farm were possible sources of A. viridans that caused infection in the cows with mastitis.

No MeSH data available.


Related in: MedlinePlus