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Characterization of Aerococcus viridans isolated from milk samples from cows with mastitis and manure samples.

Saishu N, Morimoto K, Yamasato H, Ozaki H, Murase T - J. Vet. Med. Sci. (2015)

Bottom Line: Antimicrobial susceptibilities were determined for 29 isolates from milk, processed manure and bedding materials.Of these, 26 (89.7%) were resistant to clindamycin, whereas virtually all the isolates were susceptible to 12 other antimicrobials including cefalosporins that have been used to treat bovine mastitis in Japan.In vitro, three A. viridans isolates from milk and an isolate from processed manure survived for 3 hr in Good's buffer (pH 9) at high temperature (50°C).

View Article: PubMed Central - PubMed

Affiliation: Tottori Prefectural Kurayoshi Livestock Hygiene Service Center, 2-132 Seidani-cho, Kurayoshi, Tottori 682-0017, Japan.

ABSTRACT
Thirty-eight Aerococcus viridans isolates were obtained from milk from 478 cows with clinical mastitis in a farm during the periods between November 2011 and February 2012, and between December 2012 and March 2013. Additional isolates were obtained from processed manure (a mixture of composted manure, straw and hydrated lime) and bedding materials. The processed manure was later used to cover the floor of the stalls in barns as bedding materials. The temperatures recorded in the composted and processed manure were not as high as those generally observed during satisfactory composting. To reveal the association of A. viridans in manure-related products with intramammary infection in cows, isolates were characterized by their DNA fragment patterns as determined by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Isolates obtained from milk, processed manure and bedding materials had identical DNA fragment patterns. Antimicrobial susceptibilities were determined for 29 isolates from milk, processed manure and bedding materials. Of these, 26 (89.7%) were resistant to clindamycin, whereas virtually all the isolates were susceptible to 12 other antimicrobials including cefalosporins that have been used to treat bovine mastitis in Japan. In vitro, three A. viridans isolates from milk and an isolate from processed manure survived for 3 hr in Good's buffer (pH 9) at high temperature (50°C). The results suggest that the processed manure and bedding materials in this farm were possible sources of A. viridans that caused infection in the cows with mastitis.

No MeSH data available.


Related in: MedlinePlus

SmaI-digested PFGE patterns of genome DNAs of representativeA. viridans isolates.Lanes: 1 to 14, isolates from milk samples (MK) of 14 cows in farm A between November2011 and February 2012; 15 and 16, MK of 2 cows in farm B, 17 to 22, MK of 6 cows infarm A between November 2012 and March 2013; 23 to 25, isolates from bedding materials(BM) sampled in January 2013 in farm A; 26 to 29, isolates from processed manure (PM)sampled in January 2013 in farm A. Lane M, lambda ladder. The lowercase letter beloweach of the lane numbers refers to the PFGE pattern (see RESULTS). The base sizes ofthe markers are indicated on the right of the panel.
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fig_002: SmaI-digested PFGE patterns of genome DNAs of representativeA. viridans isolates.Lanes: 1 to 14, isolates from milk samples (MK) of 14 cows in farm A between November2011 and February 2012; 15 and 16, MK of 2 cows in farm B, 17 to 22, MK of 6 cows infarm A between November 2012 and March 2013; 23 to 25, isolates from bedding materials(BM) sampled in January 2013 in farm A; 26 to 29, isolates from processed manure (PM)sampled in January 2013 in farm A. Lane M, lambda ladder. The lowercase letter beloweach of the lane numbers refers to the PFGE pattern (see RESULTS). The base sizes ofthe markers are indicated on the right of the panel.

Mentions: Pulsed-field gel electrophoresis analysis: DNAs of the 29 isolates usedfor the disk diffusion testing were analyzed by PFGE. SmaI-digestedpatterns were arbitrarily designated using lowercase letters a through l. When isolates wereconsidered related to each other because 6 or less band differences were shown among theirpatterns [15], these patterns were expedientlydesignated as a single letter. Eight different patterns (Fig. 2Fig. 2.


Characterization of Aerococcus viridans isolated from milk samples from cows with mastitis and manure samples.

Saishu N, Morimoto K, Yamasato H, Ozaki H, Murase T - J. Vet. Med. Sci. (2015)

SmaI-digested PFGE patterns of genome DNAs of representativeA. viridans isolates.Lanes: 1 to 14, isolates from milk samples (MK) of 14 cows in farm A between November2011 and February 2012; 15 and 16, MK of 2 cows in farm B, 17 to 22, MK of 6 cows infarm A between November 2012 and March 2013; 23 to 25, isolates from bedding materials(BM) sampled in January 2013 in farm A; 26 to 29, isolates from processed manure (PM)sampled in January 2013 in farm A. Lane M, lambda ladder. The lowercase letter beloweach of the lane numbers refers to the PFGE pattern (see RESULTS). The base sizes ofthe markers are indicated on the right of the panel.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591142&req=5

fig_002: SmaI-digested PFGE patterns of genome DNAs of representativeA. viridans isolates.Lanes: 1 to 14, isolates from milk samples (MK) of 14 cows in farm A between November2011 and February 2012; 15 and 16, MK of 2 cows in farm B, 17 to 22, MK of 6 cows infarm A between November 2012 and March 2013; 23 to 25, isolates from bedding materials(BM) sampled in January 2013 in farm A; 26 to 29, isolates from processed manure (PM)sampled in January 2013 in farm A. Lane M, lambda ladder. The lowercase letter beloweach of the lane numbers refers to the PFGE pattern (see RESULTS). The base sizes ofthe markers are indicated on the right of the panel.
Mentions: Pulsed-field gel electrophoresis analysis: DNAs of the 29 isolates usedfor the disk diffusion testing were analyzed by PFGE. SmaI-digestedpatterns were arbitrarily designated using lowercase letters a through l. When isolates wereconsidered related to each other because 6 or less band differences were shown among theirpatterns [15], these patterns were expedientlydesignated as a single letter. Eight different patterns (Fig. 2Fig. 2.

Bottom Line: Antimicrobial susceptibilities were determined for 29 isolates from milk, processed manure and bedding materials.Of these, 26 (89.7%) were resistant to clindamycin, whereas virtually all the isolates were susceptible to 12 other antimicrobials including cefalosporins that have been used to treat bovine mastitis in Japan.In vitro, three A. viridans isolates from milk and an isolate from processed manure survived for 3 hr in Good's buffer (pH 9) at high temperature (50°C).

View Article: PubMed Central - PubMed

Affiliation: Tottori Prefectural Kurayoshi Livestock Hygiene Service Center, 2-132 Seidani-cho, Kurayoshi, Tottori 682-0017, Japan.

ABSTRACT
Thirty-eight Aerococcus viridans isolates were obtained from milk from 478 cows with clinical mastitis in a farm during the periods between November 2011 and February 2012, and between December 2012 and March 2013. Additional isolates were obtained from processed manure (a mixture of composted manure, straw and hydrated lime) and bedding materials. The processed manure was later used to cover the floor of the stalls in barns as bedding materials. The temperatures recorded in the composted and processed manure were not as high as those generally observed during satisfactory composting. To reveal the association of A. viridans in manure-related products with intramammary infection in cows, isolates were characterized by their DNA fragment patterns as determined by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Isolates obtained from milk, processed manure and bedding materials had identical DNA fragment patterns. Antimicrobial susceptibilities were determined for 29 isolates from milk, processed manure and bedding materials. Of these, 26 (89.7%) were resistant to clindamycin, whereas virtually all the isolates were susceptible to 12 other antimicrobials including cefalosporins that have been used to treat bovine mastitis in Japan. In vitro, three A. viridans isolates from milk and an isolate from processed manure survived for 3 hr in Good's buffer (pH 9) at high temperature (50°C). The results suggest that the processed manure and bedding materials in this farm were possible sources of A. viridans that caused infection in the cows with mastitis.

No MeSH data available.


Related in: MedlinePlus