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Characterization of Aerococcus viridans isolated from milk samples from cows with mastitis and manure samples.

Saishu N, Morimoto K, Yamasato H, Ozaki H, Murase T - J. Vet. Med. Sci. (2015)

Bottom Line: Antimicrobial susceptibilities were determined for 29 isolates from milk, processed manure and bedding materials.Of these, 26 (89.7%) were resistant to clindamycin, whereas virtually all the isolates were susceptible to 12 other antimicrobials including cefalosporins that have been used to treat bovine mastitis in Japan.In vitro, three A. viridans isolates from milk and an isolate from processed manure survived for 3 hr in Good's buffer (pH 9) at high temperature (50°C).

View Article: PubMed Central - PubMed

Affiliation: Tottori Prefectural Kurayoshi Livestock Hygiene Service Center, 2-132 Seidani-cho, Kurayoshi, Tottori 682-0017, Japan.

ABSTRACT
Thirty-eight Aerococcus viridans isolates were obtained from milk from 478 cows with clinical mastitis in a farm during the periods between November 2011 and February 2012, and between December 2012 and March 2013. Additional isolates were obtained from processed manure (a mixture of composted manure, straw and hydrated lime) and bedding materials. The processed manure was later used to cover the floor of the stalls in barns as bedding materials. The temperatures recorded in the composted and processed manure were not as high as those generally observed during satisfactory composting. To reveal the association of A. viridans in manure-related products with intramammary infection in cows, isolates were characterized by their DNA fragment patterns as determined by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Isolates obtained from milk, processed manure and bedding materials had identical DNA fragment patterns. Antimicrobial susceptibilities were determined for 29 isolates from milk, processed manure and bedding materials. Of these, 26 (89.7%) were resistant to clindamycin, whereas virtually all the isolates were susceptible to 12 other antimicrobials including cefalosporins that have been used to treat bovine mastitis in Japan. In vitro, three A. viridans isolates from milk and an isolate from processed manure survived for 3 hr in Good's buffer (pH 9) at high temperature (50°C). The results suggest that the processed manure and bedding materials in this farm were possible sources of A. viridans that caused infection in the cows with mastitis.

No MeSH data available.


Related in: MedlinePlus

Numbers of milk samples of cows from which the following bacteria were isolated:A. viridans, E. coli,Streptococcus spp. other than S. agalactiae,coagulase-negative staphylococci, Pseudomonas aeruginosa and others.Horizontal lines under the months represent winter season in Japan.
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fig_001: Numbers of milk samples of cows from which the following bacteria were isolated:A. viridans, E. coli,Streptococcus spp. other than S. agalactiae,coagulase-negative staphylococci, Pseudomonas aeruginosa and others.Horizontal lines under the months represent winter season in Japan.

Mentions: Isolation of bacteria from milk samples: From 478 milk samples from cowswith clinical mastitis in farm A, 136 bacterial isolates were obtained: 44 E.coli (32.8%), 38 A. viridians (28.3%), 33Streptococcus spp. other than S. agalactiae (24.6%), 15coagulase-negative Staphylococcus (11.2%), 2 Pseudomonas aeruginosa(1.5%) and 4 Gram-negative bacteria, which were not identified to the specieslevel. A. viridans was isolated between November and March, but not in theother months (Fig. 1Fig. 1.


Characterization of Aerococcus viridans isolated from milk samples from cows with mastitis and manure samples.

Saishu N, Morimoto K, Yamasato H, Ozaki H, Murase T - J. Vet. Med. Sci. (2015)

Numbers of milk samples of cows from which the following bacteria were isolated:A. viridans, E. coli,Streptococcus spp. other than S. agalactiae,coagulase-negative staphylococci, Pseudomonas aeruginosa and others.Horizontal lines under the months represent winter season in Japan.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591142&req=5

fig_001: Numbers of milk samples of cows from which the following bacteria were isolated:A. viridans, E. coli,Streptococcus spp. other than S. agalactiae,coagulase-negative staphylococci, Pseudomonas aeruginosa and others.Horizontal lines under the months represent winter season in Japan.
Mentions: Isolation of bacteria from milk samples: From 478 milk samples from cowswith clinical mastitis in farm A, 136 bacterial isolates were obtained: 44 E.coli (32.8%), 38 A. viridians (28.3%), 33Streptococcus spp. other than S. agalactiae (24.6%), 15coagulase-negative Staphylococcus (11.2%), 2 Pseudomonas aeruginosa(1.5%) and 4 Gram-negative bacteria, which were not identified to the specieslevel. A. viridans was isolated between November and March, but not in theother months (Fig. 1Fig. 1.

Bottom Line: Antimicrobial susceptibilities were determined for 29 isolates from milk, processed manure and bedding materials.Of these, 26 (89.7%) were resistant to clindamycin, whereas virtually all the isolates were susceptible to 12 other antimicrobials including cefalosporins that have been used to treat bovine mastitis in Japan.In vitro, three A. viridans isolates from milk and an isolate from processed manure survived for 3 hr in Good's buffer (pH 9) at high temperature (50°C).

View Article: PubMed Central - PubMed

Affiliation: Tottori Prefectural Kurayoshi Livestock Hygiene Service Center, 2-132 Seidani-cho, Kurayoshi, Tottori 682-0017, Japan.

ABSTRACT
Thirty-eight Aerococcus viridans isolates were obtained from milk from 478 cows with clinical mastitis in a farm during the periods between November 2011 and February 2012, and between December 2012 and March 2013. Additional isolates were obtained from processed manure (a mixture of composted manure, straw and hydrated lime) and bedding materials. The processed manure was later used to cover the floor of the stalls in barns as bedding materials. The temperatures recorded in the composted and processed manure were not as high as those generally observed during satisfactory composting. To reveal the association of A. viridans in manure-related products with intramammary infection in cows, isolates were characterized by their DNA fragment patterns as determined by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Isolates obtained from milk, processed manure and bedding materials had identical DNA fragment patterns. Antimicrobial susceptibilities were determined for 29 isolates from milk, processed manure and bedding materials. Of these, 26 (89.7%) were resistant to clindamycin, whereas virtually all the isolates were susceptible to 12 other antimicrobials including cefalosporins that have been used to treat bovine mastitis in Japan. In vitro, three A. viridans isolates from milk and an isolate from processed manure survived for 3 hr in Good's buffer (pH 9) at high temperature (50°C). The results suggest that the processed manure and bedding materials in this farm were possible sources of A. viridans that caused infection in the cows with mastitis.

No MeSH data available.


Related in: MedlinePlus