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Microarray Analysis of the Gene Expression Profile and Lipid Metabolism in Fat-1 Transgenic Cattle.

Liu X, Bai C, Ding X, Wei Z, Guo H, Li G - PLoS ONE (2015)

Bottom Line: This analysis also identified 11 significantly enriched genes that were involved in the peroxisome proliferator-activated receptor signaling pathway.The information obtained in this study indicated that the introduction of an exogenous fat-1 gene into cattle affects the gene expression profile and the process of lipid metabolism in these animals.These results may provide important insights into how an exogenous fat-1 gene synthesizes n-3 PUFAs in transgenic cattle and other mammals.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot, China; College of Animal Science and Animal Medicine, Tianjin Agriculture University, Tianjin, China.

ABSTRACT
Long-chain n-3 polyunsaturated fatty acids (n-3 PUFAs) are beneficial for human health. However, humans and mammals are unable to synthesize n-3 PUFAs because they lack the n-3 desaturase gene fat-1 and must therefore obtain this type of fatty acid through their diet. Through the production of fat-1 transgenic animals, it is possible to obtain animal products that are rich in n-3 PUFAs, such as meat and milk. The aim of this study was to analyze the gene expression profile and the mechanism of lipid metabolism in fat-1 transgenic cattle and to accumulate important basic data that are required to obtain more efficient fat-1 transgenic cattle. Transcriptome profiling of fat-1 transgenic and wild-type cattle identified differentially expressed genes that are involved in 90 biological pathways, eight pathways of which were related to lipid metabolism processes 36 genes of which were related to lipid metabolism. This analysis also identified 11 significantly enriched genes that were involved in the peroxisome proliferator-activated receptor signaling pathway. These findings were verified by quantitative polymerase chain reaction. The information obtained in this study indicated that the introduction of an exogenous fat-1 gene into cattle affects the gene expression profile and the process of lipid metabolism in these animals. These results may provide important insights into how an exogenous fat-1 gene synthesizes n-3 PUFAs in transgenic cattle and other mammals.

No MeSH data available.


The significantly enriched genes in the ‘PPAR signaling pathway’.Red nodes indicate the significantly up-regulated genes in fat-1 transgenic cattle, and the pink nodes indicate the significantly down-regulation genes.
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pone.0138874.g003: The significantly enriched genes in the ‘PPAR signaling pathway’.Red nodes indicate the significantly up-regulated genes in fat-1 transgenic cattle, and the pink nodes indicate the significantly down-regulation genes.

Mentions: Pathway-based analysis helps to further understand the biological functions of genes. In this study, we performed KEGG analysis and obtained two significantly enriched signal pathways, including the ‘ECM-receptor interaction’ and ‘PPAR signaling pathway’ (p<0.05, FDR<0.2). The ‘PPAR signaling pathway’ is important during adipocyte tissue development and differentiation and the activation of lipogenesis [32]. It is clear that long-chain PUFAs can activate PPARs and subsequently regulate the expression of important genes that are related to lipid metabolism [33]. Therefore, we focused on the ‘PPAR signaling pathway’. We observed that there were 11 significantly differentially expressed genes enriched in the ‘PPAR signaling pathway’ (Fig 3 and S5 Table). When fat-1 transgenic cattle were compared with wild-type cattle, there was a significant (P < 0.05) increase in the expression of six genes related to lipid metabolism (ACOX1, SCP2, FABP2, CD36, SCD5, and LPL) and a significant (P < 0.05) decrease in the expression of five other genes (MMP1, CPT1B, CYP4A22, FABP3, and APOA1) (Fig 3 and S5 Table). At the same time, we found that 6 of the 11 genes (FABP3, APOA1, CPT1B, ACOX1, SCD5, LPL) were same as the 36 genes from the eight GO terms of the lipid metabolism processes obtained by GO enrichment analysis (Table 2, Table 3 and S5 Table).


Microarray Analysis of the Gene Expression Profile and Lipid Metabolism in Fat-1 Transgenic Cattle.

Liu X, Bai C, Ding X, Wei Z, Guo H, Li G - PLoS ONE (2015)

The significantly enriched genes in the ‘PPAR signaling pathway’.Red nodes indicate the significantly up-regulated genes in fat-1 transgenic cattle, and the pink nodes indicate the significantly down-regulation genes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4591129&req=5

pone.0138874.g003: The significantly enriched genes in the ‘PPAR signaling pathway’.Red nodes indicate the significantly up-regulated genes in fat-1 transgenic cattle, and the pink nodes indicate the significantly down-regulation genes.
Mentions: Pathway-based analysis helps to further understand the biological functions of genes. In this study, we performed KEGG analysis and obtained two significantly enriched signal pathways, including the ‘ECM-receptor interaction’ and ‘PPAR signaling pathway’ (p<0.05, FDR<0.2). The ‘PPAR signaling pathway’ is important during adipocyte tissue development and differentiation and the activation of lipogenesis [32]. It is clear that long-chain PUFAs can activate PPARs and subsequently regulate the expression of important genes that are related to lipid metabolism [33]. Therefore, we focused on the ‘PPAR signaling pathway’. We observed that there were 11 significantly differentially expressed genes enriched in the ‘PPAR signaling pathway’ (Fig 3 and S5 Table). When fat-1 transgenic cattle were compared with wild-type cattle, there was a significant (P < 0.05) increase in the expression of six genes related to lipid metabolism (ACOX1, SCP2, FABP2, CD36, SCD5, and LPL) and a significant (P < 0.05) decrease in the expression of five other genes (MMP1, CPT1B, CYP4A22, FABP3, and APOA1) (Fig 3 and S5 Table). At the same time, we found that 6 of the 11 genes (FABP3, APOA1, CPT1B, ACOX1, SCD5, LPL) were same as the 36 genes from the eight GO terms of the lipid metabolism processes obtained by GO enrichment analysis (Table 2, Table 3 and S5 Table).

Bottom Line: This analysis also identified 11 significantly enriched genes that were involved in the peroxisome proliferator-activated receptor signaling pathway.The information obtained in this study indicated that the introduction of an exogenous fat-1 gene into cattle affects the gene expression profile and the process of lipid metabolism in these animals.These results may provide important insights into how an exogenous fat-1 gene synthesizes n-3 PUFAs in transgenic cattle and other mammals.

View Article: PubMed Central - PubMed

Affiliation: The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot, China; College of Animal Science and Animal Medicine, Tianjin Agriculture University, Tianjin, China.

ABSTRACT
Long-chain n-3 polyunsaturated fatty acids (n-3 PUFAs) are beneficial for human health. However, humans and mammals are unable to synthesize n-3 PUFAs because they lack the n-3 desaturase gene fat-1 and must therefore obtain this type of fatty acid through their diet. Through the production of fat-1 transgenic animals, it is possible to obtain animal products that are rich in n-3 PUFAs, such as meat and milk. The aim of this study was to analyze the gene expression profile and the mechanism of lipid metabolism in fat-1 transgenic cattle and to accumulate important basic data that are required to obtain more efficient fat-1 transgenic cattle. Transcriptome profiling of fat-1 transgenic and wild-type cattle identified differentially expressed genes that are involved in 90 biological pathways, eight pathways of which were related to lipid metabolism processes 36 genes of which were related to lipid metabolism. This analysis also identified 11 significantly enriched genes that were involved in the peroxisome proliferator-activated receptor signaling pathway. These findings were verified by quantitative polymerase chain reaction. The information obtained in this study indicated that the introduction of an exogenous fat-1 gene into cattle affects the gene expression profile and the process of lipid metabolism in these animals. These results may provide important insights into how an exogenous fat-1 gene synthesizes n-3 PUFAs in transgenic cattle and other mammals.

No MeSH data available.